Studies on bacillus subtilis, as potential probiotics, on the biochemical parameters of rainbow trout, oncorhynchus mykiss (walbaum) to challenge infections

Abstract

In this study Bacillus subtilis is used for control of streptococcosis with the agent of Streptococcus iniae in Rainbow trout. The experience was carried out in 2 groups (Control and Treatment) and 3 replicates. In Control group, probiotic was not applied in diet but in Treatment group, Bacillus subtilis was administered in feed at a concentration of 10 7 cells g-1. In the day of forty five, 0.1 ml intraperitoneally injection of S.iniae with 2×10 7 cells ml-1 dosage was done for both of groups and were checked for the rest of the survey duration (2 weeks). At the end of the time which took about two months, blood samples were caught for biochemical experiments for realizing the effect of B.subtilis feeding on resistance of fish against S.iniae infection. After injection of S.iniae, there was no significant difference among Control(C) and Treatment(T) groups considering parameters such as glucose and aspartate aminotransferase (p>0.05). But significant difference was seen in the serum total protein, serum albumin, IgM, lysozyme, 38 M. Kamgar, R. Pourgholam, M. Ghiasi and M. Ghane urea, Alanin aminotransferase and alkaline phosphatase in both groups (p<0.05). The serum total protein, serum albumin, IgM and lysozyme were higher in T group and urea, Alanin aminotransferase and alkaline phosphatase were lower in comparison with the control. The results of the present study indicate that B.subtilis can be used as an agent for the control of streptococcosis in Rainbow trout hatchery and culture farms for decreasing economical disasters.

Evaluation of some haemolymph biochemical properties and F‐cell prevalence in hepatopancreas of white leg shrimp (Litopenaeus vanammei) after fed diets containing apple cider vinegar and propionic acid

“…In the present study, albumin concentration significantly improved at 2% and 4% ACV and 0.5% PA groups; meanwhile, it is slightly higher in shrimp fed 1% ACV diet compared with control diet. In confirmation described results, albumin and total protein concentrations were significantly increased in L. vannamei when fed with the diet containing Bacillus subtilis (Kamgar & Ghane, 2013). However, a raise of albumin concentration led to the hypothesis that dietary supplementation with ACV and PA improved nutritional value.…”

Variations of the intestinal gut microbiota of farmed rainbow trout, Oncorhynchus mykiss (Walbaum), depending on the infection status of the fish

“…The genus Bacillus has been identified in many studies to be a common part of the core microbial community of the fish gut (Austin and Al‐Zahrani ; Cahill, ; Ringø et al ; Kim et al ) and widely applied as a probiotic in aquaculture due to its ability to stimulate the host immune system, produce antimicrobial compounds, and other useful substances such as digestive enzymes, amino acids, etc. (Newaj‐Fyzul et al ; Wang et al ; Olmos and Paniagua‐Michel, ; Kamgar et al ).…”

Probiotic potential Streptomyces species from the grains of pearl millet (Pennisetum glaucum)

“…A neighbor joining dendrogram generated using the sequence from the selected actinomycete (1400 bp) and representative sequences from the databases revealed that it belonged to Streptomyces spp. Pearl millet flour has been used for isolation of LAB (Badau, 2006;Kamgar et al, 2013;Okoronkwo, 2014). Microbes such as Lactobacillus, Bifidobacterium and Weissella spp.…”