Studies on bovine serum albumin–sodium dodecyl sulfate complexes using pyrene fluorescence probe and 5-doxylstearic acid spin probe

Honda, Chikako; Kamizono, Hiroko; Matsumoto, Ken‐ichiro; Endo, Kazutoyo

doi:10.1016/j.jcis.2004.06.023

Cited by 53 publications

(27 citation statements)

References 36 publications

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“…We found that aggregation was detectable above a concentration of 100 nM of the cholesterol-modified RNA and increases up to at least 10 mM. This gradual aggregation over a more than 100-fold concentration range stands in contrast to the sharp phase transition during the aggregation of sodium dodecyl sulfate (SDS) micelles, at a defined critical micelle concentration (cmc) of 8 mM (Honda et al 2004). For the cholesterolmodified RNA, the presence of the polymerase ribozyme increases the aggregation concentration by only 2.2-fold (Fig.…”

Section: Resultsmentioning

confidence: 88%

Improved polymerase ribozyme efficiency on hydrophobic assemblies

Müller

Bartel²

2008

RNA

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During an early step in the evolution of life, RNA served both as genome and as catalyst, according to the RNA world hypothesis. For self-replication, the RNA organisms must have contained an RNA that catalyzes RNA polymerization. As a first step toward recapitulating an RNA world in the laboratory, a polymerase ribozyme was generated previously by in vitro evolution and design. However, the efficiency of this ribozyme is about 100-fold too low for self-replication because of a low affinity of the ribozyme to its primer/template substrate. To improve the substrate interactions by colocalizing ribozyme and substrate on micelles, we attached hydrophobic anchors to both RNAs. We show here that the hydrophobic anchors led to aggregates with the expected size of the corresponding micelles. The micelle formation increased the polymerization yield of full-length products by 3-to 20-fold, depending on substrates and reaction conditions. With the best-characterized substrate, the improvement in polymerization efficiency was primarily due to reduced sequence-specific stalling on partially extended substrates. We discuss how, during the origin of life, micellar ribozyme aggregates could have acted as precursors to membraneencapsulated life forms.

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Section: Resultsmentioning

confidence: 88%

Improved polymerase ribozyme efficiency on hydrophobic assemblies

Müller

Bartel²

2008

RNA

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“…Such a profile is also found in literature. 76 There is an initial steep rise for the Group A surfactants at pH 9⋅0 essentially due to the formation of the hydrophobic GS l n complex. At pH 5⋅4, the initial surfactant addition causes negligible enhancement in the polarity index or the hydrophobicity as the interaction is reduced significantly at the lower pH to yield a low concentration of the said complex (GS l n ).…”

Section: Surface Tensionmentioning

confidence: 99%

Physicochemistry of hexadecylammonium bromide and its methyl and ethanolic head group analogues in buffered aqueous and gelatin solution

Mitra

Moulik

2010

J Chem Sci

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In this work, the interfacial and bulk behaviour of the amphiphiles hexadecylammonium bromide and its methyl and ethanolic head group analogues in buffered aqueous and gelatin solution has been examined. The analogues are of two categories: the methyl and the combined methyl and ethanolic head group representatives are considered as Group A compounds, and all non-methyl but ethanolic head group species are taken as Group B compounds. Different physical techniques have been employed to ascertain the amphiphilic behaviour in solution. The self-aggregation of these surfactants at different pH has been studied along with pH dependent interfacial activity of gelatin. The interaction of the two categories of the surfactants with gelatin at different pH has been investigated. A scheme for this interaction at various stages of the process has been proposed. The influence of the surfactant head groups on the interaction process has been assessed.

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“…These results demonstrated the presence of mixed micelles containing SDS and BSA, in agreement with previously published results. [48][49][50] Similar AUC-SV experiments were conducted using darbepoetin alfa (5. showed that the presence of darbepoetin alfa did not affect the sedimentation profiles of the polysorbates. The PS-20 and PS-80 micelle s-values were 1.7 and 2.1s, respectively.…”

Section: Monitoring Darbepoetin Alfa-polysorbate Interactions By Sedimentioning

confidence: 99%