STUDIES ON BREEDING FOR HIGH PROTEIN CONTENT AND QUALITY IN RICE : I. Estimatron of Seed Protein Content Usming the Dye-binding Method

Osone, Kenichi; Takagi, Toshie

doi:10.1270/jsbbs1951.20.301

Cited by 5 publications

(1 citation statement)

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“…Studies on rice, however, have generally been limited to ways of increasing the total content of storage proteins (Beachell et a!., 1972;Higashi et a!., 1974;Kambayashi et a!., 1984;Osone and Takagi, 1970) or increasing the lysine content in the storage proteins (Shin et a!., 1977). However, due to the fact that the gene (s) for rice storage proteins themselves has never been identified, the qualitative improvement of the rice proteins themselves remains virtually unexplored.…”

Section: Introductionmentioning

confidence: 99%

Mutants for rice storage proteins. IV. Maternally inherited mutants for storage proteins of protein bodies in the starchy endosperm

et al. 1990

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Maternal inheritance, a phenomenon extremely rare in plants, was observed in two mutants for rice storage protein in the starchy endosperm. When these mutants, 10/13a-L and 10/16-H, were crossed reciprocally with their original variety, Kinmaze, the F1 seeds in all crosses showed the same character as the maternal parent. The F2 seeds of the reciprocal crosses between Kinmaze and 10/13a-L were also of normal type, but those between Kinmaze and 10/16-H showed 10/16-H type. In 10/13a-L, the segregation ratio of F2 plants observed in F3 seeds showed a good fit to the expected 3:1 ratio, and in 10/16-H, the segregation ratio of F2 plants observed in F3 seeds showed a good fit to the expected 1:3 ratio. From the results of F1, F2 and F3 seeds, it was concluded that the mutants were expressed according to the genetic behaviour of material inheritance governed by plant genotype, and that 10/13a-L and 10/16-H were controlled by a single recessive and dominant gene, respectively. SDS-PAGE analysis indicated that both mutant genes were regulatory genes for the storage proteins. Based on the results of allelism tests, the genes for 10/13a-L and 10/16-H were designated as esp-3 and Esp-4, respectively. Since esp-1, the gene controlling the 13b-L, a mutant of rice storage protein, produces loss of the effect of maternal inheritance of esp-3, it is assumed that esp-1 is epistatic to esp-3.

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Section: Introductionmentioning

confidence: 99%

Mutants for rice storage proteins. IV. Maternally inherited mutants for storage proteins of protein bodies in the starchy endosperm

et al. 1990

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Mutants for rice storage proteins

Kumamaru

Satoh

Iwata

et al. 1988

Theoret. Appl. Genetics

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To obtain genetic materials to breed qualitatively improved rice storage proteins, we screened about 3,000 mutant lines induced by the treatment of rice fertilized egg cell with N-methyl-N-nitrosourea (MNU). The screening was performed by comparing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with that of the original variety, Kinmaze, especially focussing on the changes in polypeptides present in two kinds of protein bodies, PB-I and PB-II. We selected 17 mutant lines and classified them into 4 types on the basis of variations of the relative contents of the polypeptides. Determination of extracted protein in the starchy endosperm of the mutants revealed changes in the content of prolamin and glutelin but not globulin. In some mutants there was marked accumulation of 57 kDa polypeptide concomitant with the remarkable reduction of glutelin subunits. Treatment of the fertilized egg cell with MNU was found to be an effective method to induce mutations for storage proteins in protein bodies of rice.

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Differences in DBC values of polyploidal mulberry (Morus sp.) leaves

Gupta

Ray

1977

Proceedings of the Indian Academy of Sciences - Section B

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STUDIES ON BREEDING FOR HIGH PROTEIN CONTENT AND QUALITY IN RICE : I. Estimatron of Seed Protein Content Usming the Dye-binding Method

Cited by 5 publications

References 9 publications

Mutants for rice storage proteins. IV. Maternally inherited mutants for storage proteins of protein bodies in the starchy endosperm

Mutants for rice storage proteins. IV. Maternally inherited mutants for storage proteins of protein bodies in the starchy endosperm

Mutants for rice storage proteins

Differences in DBC values of polyploidal mulberry (Morus sp.) leaves

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