Studies on carbohydrate-metabolizing enzymes. 10. Barley β-glucosidases

Abstract

2. The carbon dioxide ratio, i.e. the ratio of the specific activities of expired carbon dioxide during the infusion of [1-14C]acetate and [2-14C]acetate, respectively, was 1-58 ± 0-17 (3) for non-pregnant sheep, 1-66 ± 0-25 (4) for sheep in early pregnancy, 1-74 + 0-14 (4) for sheep in late pregnancy, and 1-18 ± 0-11 (3) for under-fed sheep in late pregnancy. Calculations based on the last two ratios indicate that citric acid-cycle turnover in under-fed pregnant sheep is about one-quarter of the turnover found in well-fed pregnant sheep.3. The output of carbon dioxide is similar in all four groups of animals, but the percentage of carbon dioxide derived from acetate is low in the poorly-fed sheep in late pregnancy. The amount of acetate converted into carbon dioxide is lower in these animals but, because total aceta.te utilization is low in this group, the acetate presented to the tissues is oxidized as readily as in the sheep of other groups.

Studies on carbohydrate-metabolizing enzymes. 11. The hydrolysis of lichenin by enzyme preparations from malted barley and Rhizopus arrhizus

“…), and the specific viscosity measured at intervals. /3-(1--4)-Glucosanase activities were calculated as described by Anderson et al (1964).…”

“…The methods used were those described by Anderson et al (1964). The degree of polymerization of oligosaccharides was measured: (a) from the reducing power, before and after acid hydrolysis; (b) from the weight of borohydride-reduced oligosaccharide which produced 2 mol.prop.…”

“…LICHENASES activities in preparations from Aspergillus oryzae and Helix pomatia. Anderson, Cunningham & Manners (1964) found that extracts of ungerminated barley which showed lichenase activity contained separate cellobiase, laminaribiase, fi-(1-+4)-glucosanase and ,B-(1-+3)-glucosanase activities. In the presence of 8 mMglucono-(1-÷4)-lactone all the activities except that of fi-(1--3)-glucosanase were inhibited.…”

“…* Part 10: Anderson, Cunningham & Manners (1964). LICHENASES activities in preparations from Aspergillus oryzae and Helix pomatia.…”

STUDIES ON CARBOHYDRATE METABOLIZING ENZYMES PART XXII. THE Β-Glucanase SYSTEM OF MALTED BARLEY

“…[4][5][6][7][8][9][10][11][12][13] The precipitates of protein were collected by centrifugation, dissolved in distilled water and dialysed for 60 hr. at 2°C.…”