Studies on carbohydrate-metabolizing enzymes. Trans-β-glucosylation by barley enzymes

Abstract

Recent investigations (reviewed by Gottschalk, 1958) have shown that the enzymic hydrolysis of a glycosidic linkage in an oligosaccharide is, in fact, a transglycosylation reaction in which a glycosyl group is transferred from the substrate to a water molecule. The latter thus acts as a glycosyl acceptor:

Studies on carbohydrate-metabolizing enzymes. 11. The hydrolysis of lichenin by enzyme preparations from malted barley and Rhizopus arrhizus

“…RF relative to that of glucose, 0-41 (gentiobiose) in the laminarin and cellobiose digests is attributed to trans-,-glucosylation at the disaccharide level (cf. Anderson & Manners, 1959).…”

“…The apparatus for continuous electrophoresis was that used by Cunningham, Manners & Wright (1962). Paper electrophoresis of sugars was carried out as described by Anderson & Manners (1959). The solvents used for paper chromatography were: A, ethyl acetatepyridine-water (10:4:3, by vol.…”

Studies on carbohydrate-metabolizing enzymes. 2. Trans-α-glucosylation by extracts of Tetrahymena pyriformis

“…Analytical methods. The methods used have been described by Duncan & Manners (1958) and Anderson & Manners (1959). An ethyl acetate-pyridine-water (10:4:3, by vol.)…”

“…TRANS-c-GLUCOSYLATION BY EXTRACTS OF TETRAHYMENA PYRIFORMIS* BY A. R. ARCHIBALD Aim D. J. MANNERS Department of Chemistry, University of Edinburgh (Received 6 March 1959) In continuation of studies on transglucosylation reactions (Duncan & Manners, 1958;Anderson & Manners, 1959;Duncan, Manners & Thompson, 1959) we now describe an investigation of the metabolism of maltose by cell-free extracts of the ciliate Tetrahymerna pyriformi&. The presence of a maltase in such extracts has been noted by Ryley (1952).…”

STUDIES ON CARBOHYDRATE METABOLIZING ENZYMES PART XXII. THE Β-Glucanase SYSTEM OF MALTED BARLEY

“…[4][5][6][7][8][9][10][11][12][13] The precipitates of protein were collected by centrifugation, dissolved in distilled water and dialysed for 60 hr. at 2°C.…”