Studies on cell adhesion in tissue culture

Weiss, Leonard

doi:10.1016/0014-4827(74)90707-1

Cited by 10 publications

(2 citation statements)

References 17 publications

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“…Cells with two or more nuclei can be referred to as polyploid or as binucleate (multinucleate) cells [12]. Poly ploidy is the result of a phenomenon whereby cells under go repeated cycles of DNA synthesis without entering mitosis.…”

Section: Discussionmentioning

confidence: 99%

Mitosis and the Presence of Binucleate Cells among Glomerular Podocytes in Diseased Human Kidneys

Nagata

Yamaguchi²,

Komatsu

et al. 1995

Nephron

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Podocytes are highly differentiated cells that appear to be postmitotic. However, it is unclear whether cell division or the proliferation of podocytes takes place in the setting of glomerular injury. In this study, we analyzed the presence of mitotic figures and of binucleate or multinucleate podocytes in diseased human kidneys by light and electron microscopy. Among the 164 cases analyzed, mitotic figures in podocytes were found in only one cell, in a case of focal and segmental glomerulosclerosis, and 6 cases provided evidence of two or more nuclei in a single podocyte. Serial sections by light microscopy confirmed that the nuclei in multinucleate cells were separate and of approximately the same size. Electron-microscopic analysis of the binucleate podocytes revealed hypertrophy and effacement of foot processes. These findings indicate that, in human podocytes, mitosis without cytokinesis (cell division) results in binucleate cells in cases of glomerular injury.

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Section: Discussionmentioning

confidence: 99%

Mitosis and the Presence of Binucleate Cells among Glomerular Podocytes in Diseased Human Kidneys

Nagata

Yamaguchi²,

Komatsu

et al. 1995

Nephron

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“…The studies revealed that in addition to the sialic acid carboxy groups, positively charged groups of lysine side chain e-amino groups, sulphydryl groups and alkaline phosphatase-susceptible phosphate groups may be involved in cell interactions. In addition, Weiss [34] reported that the presence of RNase-susceptible phosphate groups on cells might be dependent upon the cell cycle. Then it was tried to compare the quantities of surplus negative surface charges calculated according to von Smoluchowski from results of cell/particle electrophoresis experiments with changes of cell electrophoretic mobilities, and simultaneously with the numbers of the negatively charged chemical groups, which when removed from the cell surface by enzymatic treatment, induced the changes.…”

Section: Von Smoluchowski Formulamentioning

confidence: 99%

Electrophoresis of cells and the biological relevance of surface charge

2002

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Recent developments in electrophoresis of cells are reviewed. Problems and progress in automation and miniaturization of analytical electrophoresis instruments as well as in the interpretation of experimentally determined electrophoretic mobility (EPM) data are summarized: In recent times, the EPM determination techniques not only became more reliable and faster, but also more knowledge could be gained about the cell surface electrical properties, the structure of the glycocalyx as well as its influence on the cell peripheral regions and microenvironment by applying cell electrophoresis. In addition, ways are shown to solve discrepancies between physical requirements of a preparative cell electrophoresis procedure and the quantities of ions, which have to be dissolved in cell suspension media. As the modern machines allow the purification of untagged cells suspended in more cell friendly and physiological media, they are likely to be valuable tools in several useful practical applications in clinical transplantation, gene therapy and treatment of disease states.

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