The darkspotted numbfish, Narcine maculata (shaw), was examined for cestodes for the first time. A remarkably large number of detached proglottids was observed upon examination of the contents of the spiral intestine. closer investigation revealed that these detached proglottids represented two different hyperapolytic species, both belonging to the lecanicephalidean family Anteroporidae Euzet, 1994. the scolices and strobilae of one of these species were subsequently found deeply embedded in the mucosa of the spiral intestine. the latter species was determined to represent a new genus; the other a new species of Anteropora subhapradha, 1955. these new taxa are described herein and the familial diagnosis is emended to include this, the second genus of Anteroporidae.
MATERIALS AND METHODSA female specimen of Narcine maculata measuring 35.5 cm in total length, was collected using a bottom trawl by the Kuching Fisheries vessel K. K. Manchong, off the coast of southern Sarawak in Malaysian Borneo (02º49′01.2″N, 110º52′47.16″E), in the south china sea in April, 2004. the spiral intestine was removed, opened with a longitudinal incision, fixed in 10% buffered formalin in the field, and subsequently transferred to 70% ethanol for storage. images of the host (No. Bo-408) can be viewed in the elasmobranch host component (http://tapewormdb.uconn.edu/hosts.php) of the global cestode Database (http:// tapeworms.uconn.edu). tapeworms were removed from the spiral intestine in the laboratory. Entire worms, scolices, strobilar fragments, and detached proglottids were prepared as whole mounts for light microscopy as follows. specimens were stained with Delafield's haematoxylin, dehydrated in a graded ethanol series, cleared in methyl salicylate, and mounted on glass slides in canada balsam. specimens for serial sectioning were dehydrated in a graded ethanol series, stained with fast green, cleared in xylene, and embedded in paraffin. Serial sections were cut at 6 mm intervals with an olympus cUt4060 retracting rotary microtome, and were subsequently attached to glass slides with 10% sodium silicate, stained in Delafield's haematoxylin, counterstained in eosin, cleared in xylene, and mounted in canada balsam. semi-permanent egg mounts were prepared as follows: gravid proglottids were placed in lactophenol for 1 h, transferred to glass slides, broken open with insect pins, and mounted in lactophenol under a coverslip ringed with clear nail polish. specimens for scanning electron microscopy (sEM) were transferred A new genus and two new species of Anteroporidae (Cestoda: Lecanicephalidea) from the darkspotted numbfish, Narcine maculata (Torpediniformes: Narcinidae), off Malaysian Borneo Abstract: The cestode fauna of the darkspotted numbfish, Narcine maculata (shaw) (torpediniformes: Narcinidae), from Malaysian Borneo was examined for the first time. This work resulted in the discovery of a new genus and two new species of Anteroporidae (lecanicephalidea). Sesquipedalapex comicus gen. n., sp. n. was erected on the basis of the pecul...
