Studies on characters of immobilizing penicillin G acylase on a novel composite support PEI/SiO2

Gao, Baojiao; Wang, Xupeng; Shen, Yanling

doi:10.1016/j.bej.2005.10.007

Cited by 47 publications

(30 citation statements)

References 15 publications

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“…Based on the previous study [21], the grafting particles PEI/SiO 2 were prepared, and the typical procedure is as follows. Silica gel particles activated with the aqueous solution of methane sulfonic acid were reacted with ␥-chloropropyl trimethoxysilane (CPMS) in xylene (as solvent) at 80 • C for 6 h, resulting in the surfacemodified silica gel particles, denoted as CP-SiO 2 .…”

Section: Preparation and Characterization Of Grafting Particle Pei/siomentioning

confidence: 99%

“…The commercial PEI always is a branched macromolecule which chemical structure is given in Scheme 1, and the ratio of primary, secondary and ternary amine groups on PEI macromolecule chains is equal to 1:2:1 approximately [20]. In this work, polyethyleneimine was grafted onto the surfaces of micron-sized silica gel particles in the manner of "grafting onto" [21], and grafting particles PEI/SiO 2 were obtained. Afterward, the grafted PEI on SiO 2 particles was quaternized via two polymer reacScheme 1.…”

Section: Introductionmentioning

confidence: 99%

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Adsorption behaviour of functional grafting particles based on polyethyleneimine for chromate anions

Gao

Chen

2009

Chemical Engineering Journal

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Section: Preparation and Characterization Of Grafting Particle Pei/siomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adsorption behaviour of functional grafting particles based on polyethyleneimine for chromate anions

Gao

Chen

2009

Chemical Engineering Journal

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“…Many methods are available for enzyme immobilization, including adsorption to insoluble materials (Tomotani and Vitolo 2006), entrapment in polymeric gels (Curulli et al 2006), encapsulation in membranes (Patel et al 2006), crosslinking with bi-functional reagents (Simiddy et al 2006), or covalent linking onto insoluble carriers (Gao et al 2006). Entrapment, one of the immobilization techniques, can be defi ned as physical restriction of the enzyme within a confi ned space or network.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of laccase on polyacrylamide and polyacrylamide – κ – carragennan-based semi-interpenetrating polymer networks

Gökgöz

Altinok

2012

Artificial Cells, Blood Substitutes, and Biotechnology

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In this study, laccase enzyme (L) from Agaricus bisporus was immobilized by entrapment into polyacrylamide (PAAm) and semi-interpenetrating polymer networks (semi-IPNs) prepared with either polyacrylamide/κ-carragennan (0.05g) [PAAm/ κ-car (0.05)] or polyacrylamide/κ-carragennan (0.1 g) [PAAm/ κ-car (0.1)]. The optimum pH was 6.0 for free L, 8.0 for PAAm-L, 8.5 for PAAm/κ-car (0.05)-L, and 9.0 for PAAm/κ-car (0.1)-L. The optimum temperature was determined as 45°C for free L and 60°C for all immobilized laccases. After 27 days of storage at 4°C, free enzyme lost its initial activity whereas immobilized enzymes retained 56 % (-)80% of their initial activities. The immobilized samples were used repeatedly 35 times by retaining 28 %-58 % of their initial activity. K(m(app)) values were calculated as 0.088, 0.139, 0.133, and 0.131 mM and Vmax values were found to be 2.83 x 10(-3), 4.51×10(-3), 4.76×10(-3), and 4.97×10(-3) mM min(-1) for free L and PAAm-L, PAAm/κ-car (0.05)-L, and PAAm/κ-car (0.1)-L, respectively.

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“…However, many limitations have also been found, such as being volatilizable, very expensive, not easily available, and toxic (Talebi et al 2010;Ling et al 2011). To tackle these problems, the DTC resin here was developed by grafting polyethyleneimine (PEI) onto chloromethylated polystyrene (CPS) beads (Gao et al 2006) as the amino compound. To examine the adsorption capability, different simulated wastewater bodies with various Co(II) concentrations in the range of 40-320 mg L -1 were tested.…”

Section: Introductionmentioning

confidence: 99%

Functionalized dithiocarbamate chelating resin for the removal of Co2+ from simulated wastewater

Shi

et al. 2017

Appl Water Sci

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Industrial wastewater that contains trace amounts of heavy metal ions is often seen in petrochemical industry. While this wastewater can not be directly discharged, it is difficult to treat due to the low concentration of metal ions. Introducing chelating reagents into this wastewater for selective ion adsorption, followed by a mechanical separation process, provides an appealing solution. Toward the success of this technology, the development of effective chelating resins is of key importance. In the present work, a chelating resin containing amino and dithiocarbamate groups was reported for the removal of Co(II) metal ions in trace concentrations from simulated wastewater. By investigating the adsorption performance of the chelating resin at different solution pH values, adsorbent dosages, contact time, initial ion concentrations, and adsorption temperatures, the maximum adsorption capacity of the resin for Co(II) was identified to be 24.89 mg g -1 for a 2 g L -1 adsorbent dosage and a pH value of 5. After four adsorption-desorption cycles, 97% of the adsorption capacity of the resin was maintained. The adsorption kinetics and thermodynamics were analyzed and discussed as well.

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Studies on characters of immobilizing penicillin G acylase on a novel composite support PEI/SiO2

Cited by 47 publications

References 15 publications

Adsorption behaviour of functional grafting particles based on polyethyleneimine for chromate anions

Adsorption behaviour of functional grafting particles based on polyethyleneimine for chromate anions

Immobilization of laccase on polyacrylamide and polyacrylamide – κ – carragennan-based semi-interpenetrating polymer networks

Functionalized dithiocarbamate chelating resin for the removal of Co2+ from simulated wastewater

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