Studies on chemical induction of chromosomal aberrations in postcopulation germ cells and zygotes of female mice. I. Comparative studies on the frequency of first-cleavage chromosomal aberrations and dominant-lethal mutations.

Tanaka, Noriho

doi:10.1266/jjg.56.117

Cited by 13 publications

(4 citation statements)

References 22 publications

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“…Treatment of male rats or mice with MMS induces a dominant lethal phenotype in embryos d erived fro m th e ex pos ed sp er m (Ehling and Neuhauser-Klaus, 1990;Ashby et al, 1996) and chromosomal damage in gametes results in such a phenotype of embryos (Brewen et al, 1975;Katoh and Tanaka, 1980). Treatment of pregnant female mice with MMS at the zygote stage, however, induces fertilized eggs with degenerated chromosomes not to have aberrations at the first cleavage stage (Tanaka, 1981).…”

Section: Discussionmentioning

confidence: 99%

Differential Susceptibility of Rat Embryos to Methyl Methanesulfonate During the Pregastrulation Period

et al. 2007

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-The effects of exposure of pregnant rats to methyl methanesulfonate (MMS), an alkylating agent, during the pregastrulation period on embryonic and placental development were investigated. SD rats were treated orally with a single dose of MMS (200 mg/kg) in the morning of gestation days 0, 1, 2, 3, 4, 5, or 6 (GD0 to GD6 groups, respectively). The uterine contents including fetuses and placentas of the dams were examined on gestation day 20. The individual fetuses and placentas were weighed, and the fetuses were examined for external, visceral and skeletal anomalies. The progress of ossification was also evaluated. Both pre-and postimplantation embryonic mortalities were higher in the GD0 group than in the control group. The postimplantation loss was also increased for the GD3, GD4 and GD6 groups. Fetal malformations were rare in survivors of all the MMS-treated groups. Intrauterine growth retardation was apparent for fetuses in groups GD5 and GD6. In addition, placental weight was reduced in the GD6 group, but it was increased in the GD0 group. Effects of MMS on embryonic mortality or on fetal or placental growth were absent or minimal in the GD1 and GD2 groups. These results suggest that the susceptibility of rat embryos to MMS varies during the pregastrulation period.

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Section: Discussionmentioning

confidence: 99%

Differential Susceptibility of Rat Embryos to Methyl Methanesulfonate During the Pregastrulation Period

et al. 2007

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“…53,68,70 Chemical mutagens that are administered as chemotherapies, or consumed along with food, can also cause strand breaks in sperm DNA directly or indirectly after metabolism in the liver tissues. 38,54,73,74 These results indicate that the sites of DNA breaks which are newly formed in mature sperm exposed to radiation and chemicals are not commonly repaired by oocyte DNA repair enzymes. No clastogenic effect has been observed in human sperm exposed to microwave radiation, 75 low-frequency electromagnetic fields, 76 and dioxins 77 in vitro.…”

Section: Environmental Mutagensmentioning

confidence: 93%

“…Radiation directly breaks DNA strands in a linear dose‐dependent manner in both human and murine sperm 53,68,70 . Chemical mutagens that are administered as chemotherapies, or consumed along with food, can also cause strand breaks in sperm DNA directly or indirectly after metabolism in the liver tissues 38,54,73,74 . These results indicate that the sites of DNA breaks which are newly formed in mature sperm exposed to radiation and chemicals are not commonly repaired by oocyte DNA repair enzymes.…”

Section: Sperm Dna Damage and Transformationmentioning

confidence: 99%

DNA damage in human sperm: The sperm chromosome assay

Watanabe

2022

Reprod Medicine & Biology

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Background Sperm DNA damage is a major cause of pre‐ and post‐implantation embryonic loss in humans. However, the factors that control how and when such DNA damage occurs in human sperm are poorly understood. Methods Here, I review information relating to sperm DNA damage that can be obtained from the sperm chromosome assays described in the existing literature. Main findings The sperm chromosome assays, which consist of interspecific in vitro fertilization or intracytoplasmic sperm injection using murine oocytes and subsequent chromosome analysis, indicate that the proportion of sperm showing DNA damage is initially low and there are larger numbers of sperm with potential membrane and DNA damage that are induced after ejaculation and separation from the seminal plasma. Other assays that directly detect sperm DNA (e.g., TUNEL assays, Comet assays, and acridine orange test) are not able to distinguish and detect the initial and potential DNA damage. Furthermore, the positive values in these direct assays are influenced by the frequency of immotile sperm and amorphous sperm populations. Conclusion The findings in the sperm chromosome assays show that further improvements in sperm preparation protocols may result in the reduction of sperm DNA damage, followed by more successful outcomes in infertility treatment.

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“…The three alkylating agents used were isopropyl methanesulfonate (IPMS), N-methyl-N-nitrosourea (MNU) and dimethyl sulfate (DMS), all known for mutagenic (Ehling et al, 1972;Tanaka, 1981) and teratogenic (Koyama et al, 1970;Kalter, 1971) properties in rodents.…”

mentioning

confidence: 99%

Studies on the Relation between Alkylating Agent‐Induced Chromosomal Aberrations and Embryotoxicity in Mice. II. The Potentiation of Teratogenicity and Clastogenicity of Isopropyl Methanesulfonate, N‐Methyl‐N‐Nitrosourea and Dimethyl Sulfate by Caffeine

Nagao

Mizutani

1984

Congenital Anomalies

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The effects of caffeine on embryotoxicity including teratogenicity and clastogenicity of different classes of teratogens were examined in ICR‐mice in order to make clear the relationship between embryotoxicity and induced chromosomal aberrations in embryos. The teratogens used were isopropyl methanesulfonate (IPMS, 100 mg/kg), N‐methyl‐N‐nitrosourea (MNU, 20 mg/kg) and dimethyl sulfate (DMS, 25 mg/kg). In teratological studies, teratogens were given intraperitoneally on day 12 of gestation and caffeine subcutaneously in five divided doses (100 mg/kg for each) at 6 hours intervals during 0–24 hours following the teratogen treatment. In cyto‐genetical studies, teratogens (ip) were administered to ICR‐mice along with caffeine (sc) at a dose of 200 mg/kg on day 12 of gestation. Embryotoxicity was checked on day 18 of gestation, and clastogenicity at 6, 12, 18 and 24 hours following the co‐administration of these drugs. The co‐administration of caffeine and any one of these teratogens induced potentiative response in the formation of gross malformations and particularly in the production of chromosomal aberrations. The data obtained suggest the positive association of teratogenicity with clastogenicity of IPMS, MNU and DMS. Excess cell death due to chromosomal aberrations is highly suggestive as one of the mechanisms of embryotoxicity including teratogenicity of these teratogens.

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Studies on chemical induction of chromosomal aberrations in postcopulation germ cells and zygotes of female mice. I. Comparative studies on the frequency of first-cleavage chromosomal aberrations and dominant-lethal mutations.

Cited by 13 publications

References 22 publications

Differential Susceptibility of Rat Embryos to Methyl Methanesulfonate During the Pregastrulation Period

Differential Susceptibility of Rat Embryos to Methyl Methanesulfonate During the Pregastrulation Period

DNA damage in human sperm: The sperm chromosome assay

Studies on the Relation between Alkylating Agent‐Induced Chromosomal Aberrations and Embryotoxicity in Mice. II. The Potentiation of Teratogenicity and Clastogenicity of Isopropyl Methanesulfonate, N‐Methyl‐N‐Nitrosourea and Dimethyl Sulfate by Caffeine

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