Studies on Chronic Brucellosis: IV. An Evaluation of the Diagnostic Laboratory Tests

Evans, Alice C.; Robinson, Frank H.; Baumgartner, Leona

doi:10.2307/4582639

Cited by 17 publications

(3 citation statements)

References 15 publications

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“…This situation has been said to be particularly common in chronic brucellosis (20)(21)(22)(23). One might explain its occurrence by localization or sequestration of the Brucella in an avascular focus (23).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Coombs Test With Other Methods for Brucella Agglutinins in Human Serum 1

Hall¹,

Manion²

1953

J. Clin. Invest.

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During the past year we have compared several techniques for testing human sera for Brucella agglutinins. These have included a tube dilution method, the rapid slide method (Huddleson) and a rapid slide or screening test designed by Castaneda (1). In addition, a blocking test and a modification of the indirect Coombs test were investigated. High speed centrifugation of Brucellaserum mixtures was also used to increase the titer of Brucella agglutinins.Several unsuccessful attempts also were made to adapt the Middlebrook-Dubos (2) hemagglutination method to the detection of antibodies against Brucella. In addition, paper chromatograms were made in attempts to differentiate specific from non-specific Brucella agglutinins by the method of Hess and Roepke (3).Sera from 104 adults were studied. Of these subjects, 27 had brucellosis proved by culture, 28 had presumptive evidence of brucellosis, and 49 had no evidence of infection with Brucella and were classed as "normal" controls. METHODSVenous blood was obtained aseptically and allowed to clot in a sterile glass tube in a refrigerator. The serum was separated within a few hours. It was not heatinactivated but was tested immediately or stored in a deep-freeze cabinet at O°C. until tested.Tube dilution agglutination method. The agglutinin titer was determined by a modification of the method of Fitch, Donham, Bishop, and Boyd (4). The serum was diluted two-fold serially from 1: 10 to 1: 2,560 with 0.85 per cent saline solution. Brucella abortus antigen (0.5 ml.) was added to 0.5 ml. of saline (control) and 0.5 ml. of each dilution of the serum in separate tubes.' The

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Section: Discussionmentioning

confidence: 99%

Comparison of the Coombs Test With Other Methods for Brucella Agglutinins in Human Serum 1

Hall¹,

Manion²

1953

J. Clin. Invest.

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“…On the other hand, unlike the Mantoux test, a negative result cannot be taken to preclude the diagnosis. Evans et al (1938). found that the skin test was negative in 39% of affected patients.…”

Section: Case Reportsmentioning

confidence: 99%

Brucellosis in Children

Wallis¹

1957

BMJ

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“…The direct agglutination test has been generally agreed to be the most useful laboratory aid when cultures are negative (Evans, Robinson & Baumgartner, 1938; Spink, 1956;Dalrymple-Champneys, 1960; Joint FAO/WHO Expert Committee on Brucellosis, 1964), but unfortunately it has its limitations and its interpretation is not universally agreed.…”

Section: Introductionmentioning

confidence: 99%

The relevance of the anti-human globulin (Coombs) test and the complement-fixation test in the diagnosis of brucellosis

McDevitt

1970

J. Hyg.

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SUMMARYThe relationship between the serological findings for brucellosis and the epidemiological factors has been studied in veterinary surgeons in Northern Ireland. The anti-human globulin (Coombs) test and the complement-fixation test for brucella were used in two groups of veterinary surgeons, those self-employed and those employed by the Ministry of Agriculture.Significant serological differences were found to exist between the two groups. Those in private practice showed changes related to age, cattle skin rash, reactions to S. 19 vaccine accidents and symptoms suggestive of brucellosis in the past or the last year. But those working for the Ministry only showed titre changes related to the length of their private practice experience before joining the Ministry. In neither group was there a relationship between serological findings and the type of milk drunk or any particular group of symptoms suggestive of brucellosis.The findings indicate that high titres to brucella by the Coombs and complement-fixation test can occur in people repeatedly exposed to infection at work. Titres which would be of diagnostic importance in the rest of the population may be of little diagnostic significance even when they are as high as 160 Coombs and 128 complement fixation.

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Studies on Chronic Brucellosis: IV. An Evaluation of the Diagnostic Laboratory Tests

Cited by 17 publications

References 15 publications

Comparison of the Coombs Test With Other Methods for Brucella Agglutinins in Human Serum 1

Comparison of the Coombs Test With Other Methods for Brucella Agglutinins in Human Serum 1

Brucellosis in Children

The relevance of the anti-human globulin (Coombs) test and the complement-fixation test in the diagnosis of brucellosis

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