Studies on collagen

Venkataraman, S.

doi:10.1007/bf03049146

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Physical Properties1

Mechanism Of Extraction Of Cartilage Protein-polysaccharide By Inorganic Salt Solutions1

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1963

1973

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Cited by 4 publications

(2 citation statements)

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“…Guthrie, et al ( 88), assigned the bands at 625 and 660 cm-1 to the C-S stretching vibration of ethylene sulfide. The force constants of ethylene sulfide have been calculated from spectroscopic data and have been found to be very similar to the known constants of ethylene oxide (248).…”

Section: Physical Propertiesmentioning

confidence: 81%

Thiiranes

Sander¹

1966

Chem. Rev.

260

101

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Section: Physical Propertiesmentioning

confidence: 81%

Thiiranes

Sander¹

1966

Chem. Rev.

260

101

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“…However, CaCl2 has been shown to induce large changes in the optical rotation of collagen (Vankataraman, 1960), andAnderson &Sajdera (1971) obtained ultrastructural evidence of disaggregation ofcollagen fibrils in bovine nasal cartilage after extraction with 1.9M-CaCI2. Finally it is known that many proteins, particularly of the globular variety, have a net free energy of structure stabilization of only 42-84kJ/mol (10-20kcal/mol) (Timasheff, 1970).…”

Section: Mechanism Of Extraction Of Cartilage Protein-polysaccharide By Inorganic Salt Solutionsmentioning

confidence: 99%

Extraction of cartilage protein–polysaccharides with inorganic salt solutions

Mason

Mayes

1973

Biochemical Journal

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1. Bovine nasal cartilage was extracted with inorganic salt solutions of various ionic strengths. The efficiency of extraction of protein-polysaccharide from the tissue was determined for each extraction. The results confirm and enlarge earlier observations (Sajdera & Hascall, 1969). 2. The chloride salts of lanthanide metals extract high yields of protein-polysaccharide from the tissue at much lower concentrations than was achieved with univalent and bivalent salts. 3. The lanthanum salt of extracted protein-polysaccharide precipitates when the concentration of LaCl(3) is decreased. Precipitation is complete in the presence of 0.05m-LaCl(3). This finding is relevant to the interpretation of earlier observations on the effect of LaCl(3) on elastic recovery of articular cartilage after compression (Sokoloff, 1963). 4. A linear relationship was found between the concentration at which a particular salt is maximally effective in solubilizing protein-polysaccharide from the tissue and the enthalpy of hydration of the cation of the salt. On the basis of this relationship a hypothesis is proposed to explain the characteristic protein-polysaccharide-extraction profiles exhibited by inorganic salt solutions.

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Verlauf der Quellung bei Kollagen

Nemetschek

1963

Kolloid-Z.u.Z.Polymere

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Studies on collagen

Cited by 4 publications

References 8 publications

Thiiranes

Thiiranes

Extraction of cartilage protein–polysaccharides with inorganic salt solutions

Verlauf der Quellung bei Kollagen

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