Studies on Copper Metabolism. Ix. The Transportation of Copper in Blood 1

Gubler, C. J.; Lahey, M. Eugene; Cartwright, G. E.; Wintrobe, M. M.

doi:10.1172/jci102752

Cited by 191 publications

(57 citation statements)

References 16 publications

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“…In both the normal subject and the patient with the nephrotic syndrome, within the limits of experimental error all. of the injected copper appeared in the direct-reacting fraction of the plasma copper (11 Prior to the administration of various substances this ratio was relatively constant from day to day in each of the six adult patients. This is illustrated in a single patient (C. E.) in Figure 3.…”

Section: Methodsmentioning

confidence: 90%

“…First, the decrease in the plasma concentration of copper and iron appeared to be associated with a decrease in the concentration of both ceruloplasmin and transferrin. Thus, since approximately 96 per cent of all of the copper in plasma is normally present as ceruloplasmin (1,11), in all of the patients with a marked reduction in the plasma copper level, it is probable that there was a reduction in the ceruloplasmin concentration. That the concentration of the transferrin was reduced is indicated by the recorded values for total-iron binding capacity of the plasma.…”

Section: Discussionmentioning

confidence: 99%

“…The methods employed have been described in other communications; for the measurement of red blood cell and plasma copper (10); that for the direct-reacting copper fraction of plasma (11); for plasma iron (12); and for total iron-binding capacity of the plasma (13). Total serum protein, albumin and globulin were determined by the biuret method (14) with the modification of Weichselbaum (15).…”

Section: Methodsmentioning

confidence: 99%

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Studies on Copper Metabolism. XI. Copper and Iron Metabolism in the Nephrotic Syndrome 1

Cartwright¹,

Gubler²,

Wintrobe³

1954

J. Clin. Invest.

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124

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The metabolism of copper and iron in the nephrotic syndrome has received little attention. In view of the fact that copper in the plasma is bound almost exclusively to an a2-globulin (ceruloplasmin) with a molecular weight of approximately 151,000 (1, 2) while iron is bound to a #l,-globulin (transferrin) with a molecular weight of about 90,000 (3), it would seem not unlikely that these two metal-binding proteins might be excreted in large quantities in the urine of patients with pronounced proteinuria. If this loss were great and extended over a prolonged period, it is conceivable that depletion of the body stores of these two elements might occur or the capacity of the body to synthesize these two proteins might be exceeded, with the consequence that the plasma level of ceruloplasmin and transferrin would be reduced.In the course of investigations in this laboratory concerning the anemia associated with infection (4), a patient was studied who, while afflicted with chronic osteomyelitis, developed the nephrotic syndrome. In association with the severe hypoproteinemia and proteinuria, the plasma copper level decreased from 246 ug. per 100 ml., the high levels usually found with infection, to 40 JUg. per 100 ml. in'spite of the continued presence of osteomyelitis. More recently (5) we observed hypocupremia in two of three children with the nephrotic syndrome. Munch-Petersen (6) has reported that the urinary excretion of copper is increased in patients with proteinuria and that the amount of copper excreted is greatest in those with the highest concentration of protein in the urine. Although he did not record the plasma copper values he stated that there was no correlation between the plasma copper concentration and the amount of copper in the urine.

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Section: Methodsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Studies on Copper Metabolism. XI. Copper and Iron Metabolism in the Nephrotic Syndrome 1

Cartwright¹,

Gubler²,

Wintrobe³

1954

J. Clin. Invest.

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124

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“…They suggested that the copper remaining in the albumin fraction might be attached to globulin which could not be completely separated from the albumin by ammonium sulfate precipitation. More recently, in our laboratory (5), it was demonstrated that there is a small fraction of the plasma copper (0 to 17 per cent) in normal subjects which reacts directly 1 This investigation was supported by a research grant (C-2231) from the National Cancer Institute, National Institutes of Health, Public Health Service. 2 Public Health Research Fellow of the National Heart Institute.…”

mentioning

confidence: 91%

“…Holmberg and Laurell (4,10) demonstrated that in such patients most of the copper is associated with the globulin fraction and that there is a high degree of correlation between the copper content of the sera and the oxidase activity. In pregnant women, the hypercupremia is due mainly to an increase in ceruloplasmin (11); although there is a modest increase in the direct-reacting fraction as well (5).…”

mentioning

confidence: 99%