Studies on Corynebacterium pseudotuberculosis, Staphylococcus aureus and Staphylococcus aureus subsp. anaerobius isolated from sheep skin abscesses in Beni Suef Governorate.

Afifi, Samia I.

doi:10.21608/jvmr.2020.77592

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…Culture conditions in Mueller Hinton (MH) were tested and adapted from the literature [22,23], serial dilution, and MIC and MBC procedure followed CLSI 2018 guidelines [24], and previous description of ddATP antibacterial action and its MIC values for other bacterial species was considered to establish a starting concentration [18]. In summary, initial experiments were conducted with ddATP diluted into a 16 µmol/mL solution of MH broth with 0.2% of Tween 80, followed by serial dilution to 50% of the concentration from 16 to 0.001 µmol/mL in a 96-well flat-bottom plate.…”

Section: Minimum Inhibitory Concentration and Minimum Bactericidal Co...mentioning

confidence: 99%

Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis

et al. 2022

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Caseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2',3'-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosisinduced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections.

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Section: Minimum Inhibitory Concentration and Minimum Bactericidal Co...mentioning

confidence: 99%

Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis

et al. 2022

View full text Add to dashboard Cite

show abstract

Studies on Corynebacterium pseudotuberculosis, Staphylococcus aureus and Staphylococcus aureus subsp. anaerobius isolated from sheep skin abscesses in Beni Suef Governorate.

Cited by 1 publication

References 15 publications

Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis

Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis

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