Studies on cytoplasmic ribonucleic acid from rat liver

Bosch, L.; Wende, G. van der; Sluyser, M.; Bloemendal, H.

doi:10.1016/0006-3002(61)90792-2

Cited by 16 publications

(2 citation statements)

References 15 publications

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“…Yeast s-RNA was prepared by the method of Monier et al (1960). In order to obtain RNA free from nucleotides and oligonucleotides, the RNA preparations were chromatographed over columns (30 X 2 cm) of Sephadex G50 (Pharmacia AB., Uppsala, Sweden) (compare Bosch et al, 1961). Water (twice distilled in aE experiments) was used as eluent and fractions (5 ml) 4-10 were coEected and used in the experiments.…”

Section: Methodsmentioning

confidence: 99%

Methylation and Breakdown of Microsomal and Soluble Ribonucleic Acid from Rat Liver by Diazomethane

Kriek¹,

Emmelot²

1963

Biochemistry

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Section: Methodsmentioning

confidence: 99%

Methylation and Breakdown of Microsomal and Soluble Ribonucleic Acid from Rat Liver by Diazomethane

Kriek¹,

Emmelot²

1963

Biochemistry

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“…We have found that 0.5 m NaCl was more effective in removing amino acid acceptor RNA from sucrose-washed microsomes than was 0.3% deoxy chola te (Table III). Bosch et al (1961) failed to find amino acid acceptor RNA in their microsomal RNA preparations; however, a probable explanation can be offered.6…”

Section: Discussionmentioning

confidence: 98%

Studies on the Amino Acid Acceptor RNA in Washed Liver Microsomes^*

Smith¹

1962

Biochemistry

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The amount of amino acid acceptor RNA found associated with the microsomes (or ribosomes) depends upon the nature of the medium used in their isolation and upon the time used for their sedimentation. When homogenates of mouse liver were sedimented for varying times at 105,000 X g and the supernatant fraction assayed for RNA and protein content and for its ability to bind amino acids to RNA, it was found that the amino acid acceptor RNA's were not "soluble" but were sedimented out of solution and at different rates. The post-microsomal particles and microsomes can be aggregated with increasing concentrations of MgCl2 up to about 0.015 M and then added MgCl2 causes disaggregation and loss of sedimentation properties. At 0.015 M MgCl2 about half of the RNA normally found in the soluble fraction is sedimented by one hour at 105,000 X g. The ability of the remaining RNA to accept amino acids is increased by only about 20%, and this fact indicates that the sedimented RNA also contains a significant amount of amino acid acceptor activity. The amount of amino acid acceptor RNA associated with the microsomes due to the above conditions can be modified to some extent by the nature of subsequent washing steps. These have included multiple washings with 0.25 M sucrose-0.001 M MgCl2, and treatment with 0.3% deoxycholate or with 0.5 M NaCl. However, no conditions have been found for the complete removal of all of the amino acid acceptor activity. The amino acid acceptor RNA that cannot be washed free from the microsomes does not appear to be due to simple contamination by soluble RNA since the ratios of the acceptor activities of this RNA for leucine, valine, tyrosine, and methionine are different from those found for soluble RNA. Also, the amino acid acceptor RNA from the microsomes differs chromatographically from the majority of the soluble RNA and is more similar to a large portion of that found in the post-microsomal fraction. Microsomal RNA freed of amino acid acceptor RNA (by chromatography) still contains 7% of its uridylic acid as the 5-ribosyl isomer. It thus appears extremely difficult (if not impossible) to completely wash microsomes (or ribosomes) free of amino acid acceptor RNA.

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Stofftrennung durch Chromatographie an porösen Gelen

Determann

1964

Angewandte Chemie

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Tabelle 3. Homolytische Spaltung von Disulfiden durch Cyanisopropyl-Radikale oder durch Makroradikale be i der Styrol-Polyinerisation. Disulfid Spaltung [%I durch Cyanisopropyl-Radikale WI la1 K ( "C) [b] bei der Styrol-Polymerisation 117,181 11.8 16,s 31 13,3 S,8 1,2 5 3 I l 2 0,14(99) 0,06(50) 0,33(99) icl O,ll(SO) 0,01(99) 0,005(99) [d] [*I Erweiterte Fassung yon Vortragen: Farbwerke Hoechst, Frankfurt(Main)-Hochst, Dezember 1962; Osterreichische Gesellschaft fur Physiologische Chemie, Wien, Februar 1963 ; Kolloid-Gesellschaft, Bad Oeynhausen, Oktober 1963. [**I In dieser Arbeit werden Molekulgewichte und -groBen als proportional angenommen. [I] Zusammenfassungen iiber Teilgebiete: P. Flodin, Dissertation, Universitat Uppsala (Schweden), 1962 [zu erhalten bei A. B. Pharmacia, Uppsala (Schweden)] ; J. Porath, Advances Protein schon langer bekannte Molekularsiebe dar, in denen durch die Raumnetz-Struktur der SiO4-und A104-Tetraeder Hohlraume (etwa 50 % des Volumens) bestehen, die durch Poren verbunden sind. Solche heute synthetisch hergestellte Zeolithe werden z. B. bei der Gastrocknung oder der Abscheidung von Olefinen aus Gasgemischen verwendet, sie haben auch bei extremen Temperaturen eine spezifische Affinitat zu polaren Stoffen. Die erste allgemeiner anwendbare Methode zur Trennung groBer Molekule von kleinen Molekulen, die Di-

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Studies on cytoplasmic ribonucleic acid from rat liver

Cited by 16 publications

References 15 publications

Methylation and Breakdown of Microsomal and Soluble Ribonucleic Acid from Rat Liver by Diazomethane

Methylation and Breakdown of Microsomal and Soluble Ribonucleic Acid from Rat Liver by Diazomethane

Studies on the Amino Acid Acceptor RNA in Washed Liver Microsomes^*

Stofftrennung durch Chromatographie an porösen Gelen

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Studies on cytoplasmic ribonucleic acid from rat liver

Cited by 16 publications

References 15 publications

Methylation and Breakdown of Microsomal and Soluble Ribonucleic Acid from Rat Liver by Diazomethane

Methylation and Breakdown of Microsomal and Soluble Ribonucleic Acid from Rat Liver by Diazomethane

Studies on the Amino Acid Acceptor RNA in Washed Liver Microsomes*

Stofftrennung durch Chromatographie an porösen Gelen

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Studies on the Amino Acid Acceptor RNA in Washed Liver Microsomes^*