Studies on Destruction of Red Blood Cells

Studies on Destruction of Red Blood Cells. Ii. Chronic Hemolytic Anemia With Paroxysmal Nocturnal Hemoglobinuria: Certain Immunological Aspects of the Hemolytic Mechanism With Special Reference to Serum Complement 1

“…Hemolysis of patients' red blood cells suspended in fresh serums in vitro did not necessarily occur at the natural pH of serum (1,2) but was always observed when the acidity of serum was increased by addition of certain acids. For this reason the hemolysis test was performed with acidified serum unless otherwise noted.…”

“…In previous publications (1,2), certain prominent features of the mechanism of hemolysis in vitro have been described for patients with the syndrome of chronic hemolytic anemia with paroxysmal nocturnal hemoglobinuria, Marchiafava-Micheli disease (3,4,5). The erythrocytes from the 5 patients studied (2) were hemolyzed by all fresh human serums of compatible blood groups, the degree of hemolysis being increased by acidification.…”

“…The erythrocytes from the 5 patients studied (2) were hemolyzed by all fresh human serums of compatible blood groups, the degree of hemolysis being increased by acidification. Patients' serums, however, produced no hemolysis of normal erythrocytes.…”

“…For the sake of simplicity, the data are presented in following order: (1) examination of serum for the presence of hemolytic antibody, (2) relation of complement to the mechanism of hemolysis, (3) antigenic properties of patient's and normal red blood cells, (4) examination of patient's red blood cells for hemolytic antibody, (5) comparison with human isohemolysins, (6) susceptibility of patient's erythrocytes to hemolysis in immunological systems, and (7) susceptibility to hemolysis in non-immunological systems. The case numbers correspond with those employed in the previous publication in which case reports were given (2).…”

“…The case numbers correspond with those employed in the previous publication in which case reports were given (2). GENERAL …”