Studies on diversity of Vibrio sp. and the prevalence of hapA, tcpI, st, rtxA&amp;C, acfB, hlyA, ctxA, ompU and toxR genes in environmental strains of Vibrio cholerae from Port Blair bays of South Andaman, India

Meena, Balakrishnan; Anburajan, Lawrance; Sathish, T.; Das, Apurba Kumar; Vinithkumar, Nambali Valsalan; Kirubagaran, Ramalingam; Dharani, G.

doi:10.1016/j.marpolbul.2019.05.011

Cited by 12 publications

(14 citation statements)

References 34 publications

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“…49 Meena et al also reported that 46.5% of V. cholerae strains isolated from water samples in India carried the hapA gene. 50 The MSHA is very important for surface colonization of V. cholerae. 51 In this study, the mshA gene was detected positive in about 6.0% of the V. cholerae isolates, which was higher than that (0.8%) reported by Xu et al, 18 but lower than that (98.8%) by Rahman et al 52 The PilA, belonging to the putative Type IV pilus of V. cholerae, is a virulence factor for Vibrio vulnificus.…”

Section: Discussionmentioning

confidence: 99%

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Pan

Liang

et al. 2020

Microbial Drug Resistance

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Vibrio cholerae is a waterborne bacterium and can cause epidemic cholera disease worldwide. Continuous monitoring of V. cholerae contamination in aquatic products is imperative for assuring food safety. In this study, we determined virulence, antimicrobial susceptibility, heavy metal tolerance, and genomic fingerprints of 370 V. cholerae isolates recovered from 12 species of commonly consumed aquatic products collected from July to September of 2018 in Shanghai, China. Among the species, Leiocassis longirostris, Ictalurus punetaus, Ophiocephalus argus Cantor, and Pelteobagrus fulvidraco were for the first time detected for V. cholerae. Toxin genes ctxAB, tcpA, ace, and zot were absent from all the V. cholerae isolates. However, high occurrence of virulence-associated genes was detected, such as hapA (82.7%), hlyA (81.4%), rtxCABD (81.4%, 24.3%, 80.3%, and 80.8%, respectively), and tlh (80.5%). Approximately 62.2% of the 370 V. cholerae isolates exhibited resistance to streptomycin, followed by ampicillin (60.3%), rifampicin (53.8%), trimethoprim (38.4%), and sulfamethoxazole-trimethoprim (37.0%). Moreover, *57.6% of the isolates showed multidrug resistant phenotypes with 57 resistance profiles, which was significantly different among the 12 species (multiple antimicrobial resistance index, p < 0.001). Meanwhile, high incidence of tolerance to heavy metals Hg 2+ (69.5%), Ni 2+ (32.4%), and Cd 2+ (30.8%) was observed among the isolates. The enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)-based fingerprinting profiles classified the 370 V. cholerae isolates into 239 different ERIC-genotypes, which demonstrated diverse genomic variation among the isolates. Overall, the results in this study meet the increasing need of food safety risk assessment of aquatic products.

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Section: Discussionmentioning

confidence: 99%

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Pan

Liang

et al. 2020

Microbial Drug Resistance

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“…Moreover, no false positive amplification of the targeted genes of V. cholerae was observed in the closely related Vibrio species, as well as in non-targeting pathogenic bacteria tested, indicating high specificity of the designed primers targeting virulence-associated genes of V. cholerae. High occurrence of V. cholerae isolates carrying target genes has been reported (Meena et al, 2019;Xu et al, 2019), e.g., hapA (95.0%), and tlh (76.0%) in the fish species (Xu et al, 2019). Since the target genes can be present in many non-pathogenic strains of V. cholerae, cytotoxicity or enterotoxicity experiments may be performed to further justify pathogenesis of target genes-positive V. cholerae strains.…”

Section: Discussionmentioning

confidence: 99%

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

Chen

et al. 2019

Front. Microbiol.

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“…However, all strains of V. parahaemolyticu, except those that bear the two pathogenic genes tdh and trh do not pose a threat to public health [37]. Among all known V. cholerae serotypes, only two (O1 and O139) possess the virulence genes required to cause diseases in public health [38,39]. Transmission of V. cholerae from environmental reservoirs to humans is associated with contaminated seawater or raw seafood consumption [39].…”

Section: Introductionmentioning

confidence: 99%

“…Among all known V. cholerae serotypes, only two (O1 and O139) possess the virulence genes required to cause diseases in public health [38,39]. Transmission of V. cholerae from environmental reservoirs to humans is associated with contaminated seawater or raw seafood consumption [39]. Genes associated with disease pathogenesis in humans are ctxA, ompU, and toxR [39].…”

Section: Introductionmentioning

confidence: 99%

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How Safe to Eat Are Raw Bivalves? Host Pathogenic and Public Health Concern Microbes within Mussels, Oysters, and Clams in Greek Markets

Lattos

Chaligiannis

Papadopoulos

et al. 2021

Foods

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Raw-bivalves consumption is a wide trend in Mediterranean countries. Despite the unambiguous nutritional value of seafood, raw consumption of bivalves may involve risks that could pose a significant threat to consumers’ health. Their filter-feeding behavior is responsible for the potential hosting of a wide variety of microorganisms, either pathogenic for the bivalves or public health threats. Under this prism, the current study was conducted in an effort to evaluate the risk of eating raw bivalves originating from the two biggest seafood markets in Thessaloniki, the largest production area of bivalves in Greece. Both microbiological and molecular methodologies were applied in order to assess the presence of various harmful microbes, including noroviruses, Bonamia, Marteilia, Esherichia coli, Salmonella, and Vibrio. Results indicated the presence of several Vibrio strains in the analyzed samples, of which the halophilic Vibrio harveyi was verified by 16S rRNA sequencing; other than this, no enteropathogenic Vibrio spp. was detected. Furthermore, although Esherichia coli was detected in several samples, it was mostly below the European Union (EU) legislation thresholds. Interestingly, the non-target Photobacterium damselae was also detected, which is associated with both wound infections in human and aquatic animals. Regarding host pathogenic microorganisms, apart from Vibrio harveyi, the protozoan parasite Marteilia refrigens was identified in oysters, highlighting the continuous infection of this bivalve in Greece. In conclusion, bivalves can be generally characterized as a safe-to-eat raw food, hosting more bivalve pathogenic microbes than those of public health concern.

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Studies on diversity of Vibrio sp. and the prevalence of hapA, tcpI, st, rtxA&C, acfB, hlyA, ctxA, ompU and toxR genes in environmental strains of Vibrio cholerae from Port Blair bays of South Andaman, India

Cited by 12 publications

References 34 publications

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

How Safe to Eat Are Raw Bivalves? Host Pathogenic and Public Health Concern Microbes within Mussels, Oysters, and Clams in Greek Markets

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