Studies on dried peas. I. —The determination of phytate phosphorus

Holt, R. B.

doi:10.1002/jsfa.2740060303

Cited by 44 publications

(17 citation statements)

References 6 publications

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“…The added Fe 3+ also reduced the mineralization of the manure native PHP fraction at cation to IP6‐P mole ratios ≥ 3:6. The high affinity of IP6 for Fe 3+ and low solubility of iron IP6 were the basis of early analytical methods for determining IP6 with FeCl 3 (Young, 1936; Holt, 1955). In previous studies, Fe 3+ and Al 3+ salts were effective in aggregating dairy manure solids, and that efficacy was shown to increase with manure TS concentration (Dao and Daniel, 2002).…”

Section: Resultsmentioning

confidence: 99%

Polyvalent Cation Effects on myo‐Inositol Hexakis Dihydrogenphosphate Enzymatic Dephosphorylation in Dairy Wastewater

Dao

2003

J of Env Quality

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Information is needed on organic polyphosphates such as myo-inositol 1,2,3,5/4,6-hexakis dihydrogenphosphate or phytate (IP6) contribution to the sources and sinks of dissolved phosphorus (PO4-P) in the soil-manure-water system. Effects of Na+, Ca2+, Al3+, and Fe3+ and cation to IP6-P mole ratios on the enzymatic dephosphorylation of IP6 were studied to determine controlling mechanisms of dephosphorylation and persistence in manure. Phytate- and PO4-P were analyzed by high-performance liquid chromatography. Phytate dephosphorylation by Aspergillus ficuum (Reichardt) Henn. phytase EC 3.1.3.8 decreases by 50 +/- 3.6 and 40 +/- 4% at pH 4.5 and 6, respectively, as Ca2+ concentrations increase and cation to IP6-P mole ratios reach 6:6. Polyanionic IP6 has a high affinity for Al3+ and Fe3+ and reductions in dephosphorylation average 27 and 32% at a cation to IP6-P mole ratio of 1:6 for Al3+ and Fe3+, respectively, while reaching more than 99% at a mole ratio of 6:6. A phytase-hydrolyzable phosphorus (PHP) fraction is native to ruminant animal manure and is proportional to total solids (TS) concentration in 1 to 100 g L(-1) suspensions. Added phytase, in effect, increases water-extractable P content of manure and the risk of environmental P dispersion. As the bioavailability and ecological effect of IP6-P appear to be regulated not only by pH-controlled enzyme activity but also by the associated counterions, the differential protective effects of cations influence the accuracy of manure PHP fraction estimates and increase phytate resistance to enzymatic dephosphorylation that may lead to its persistence in manure.

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Section: Resultsmentioning

confidence: 99%

Polyvalent Cation Effects on myo‐Inositol Hexakis Dihydrogenphosphate Enzymatic Dephosphorylation in Dairy Wastewater

Dao

2003

J of Env Quality

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“…Research into Ins phosphates in plants first concentrated on measuring levels in seeds, mainly of InsP 6 (Holt, 1955;Asada et al, 1969;Griffiths and Thomas, 1981;, and on the investigation of reaction kinetics of the enzymes involved in synthesis of InsP 6 (Phillippy et al, 1994;Phillippy, 1998;Brearley and Hanke, 2000;Stevenson-Paulik et al, 2002). From a human nutrition perspective, high InsP 6 in grain is considered undesirable due to its binding of essential micronutrient metals.…”

mentioning

confidence: 99%

Phytic Acid Synthesis and Vacuolar Accumulation in Suspension-Cultured Cells of Catharanthus roseus Induced by High Concentration of Inorganic Phosphate and Cations

et al. 2005

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We have established a new system for studying phytic acid, myo-inositol hexakisphosphate (InsP 6 ) synthesis in suspensioncultured cells of Catharanthus. InsP 6 and other intermediates of myo-inositol (Ins) phosphate metabolism were measured using an ion chromatography method. The detection limit for InsP 6 was less than 50 nM, which was sufficient to analyze Ins phosphates in living cells. Synthesis of Ins phosphates was induced by incubation in high inorganic phosphate medium. InsP 6 was mainly accumulated in vacuoles and was enhanced when cells were grown in high concentration of inorganic phosphates with the cations K 1

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“…The modified Holt procedure (1955) [8] adopted routinely in our laboratories for phytate contents determination was as follows: 0.2 − 0.5 mL of the filtrate or standard sodium phytate solution (0.2 mmol L −1 ) was diluted with distilled water to a final volume of 1.4 mL. Then, 1.0 mL of a solution of ferric solution containing 50 µg mL −1 Fe 3+ was added.…”

Section: Absorptiometric Methodsmentioning

confidence: 99%

“…These methods are based on the precipitation of ferric ion with phytate in dilute acid solution and analysis of phosphorus or iron in the precipitate [8,9]. One of the disadvantages of the iron precipitation methods is that not only inositol hexaphosphate is precipitated, but other phosphorus-containing compounds able to precipitate with ferric ion (i.e.…”

Section: Introductionmentioning

confidence: 99%

Comparison of High-Performance Ion Chromatography and absorptiometric methods for the determination of phytic acid in food samples

Talamond

Guyot

Lape³

et al. 1998

Analusis

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Abstract. The objective of this paper consists in defining the interest of a new high-performance ion chromatography method (HPIC) with chemically suppressed conductivity detector for phytic acid determination in food samples. Firsly, accuracy and precision of the HPIC method have been measured. Secondly, The HPIC method and a classical absorptiometric method were compared. The HPIC method was more sensitive and selective than the absorptiometric method which leaded to an 27% overestimation of the phytic acid content in legume seeds. Because of it is rapid and easy to perform, the HPIC method appears to be particularly suitable for routine analysis of food samples.

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Studies on dried peas. I. —The determination of phytate phosphorus

Cited by 44 publications

References 6 publications

Polyvalent Cation Effects on myo‐Inositol Hexakis Dihydrogenphosphate Enzymatic Dephosphorylation in Dairy Wastewater

Polyvalent Cation Effects on myo‐Inositol Hexakis Dihydrogenphosphate Enzymatic Dephosphorylation in Dairy Wastewater

Phytic Acid Synthesis and Vacuolar Accumulation in Suspension-Cultured Cells of Catharanthus roseus Induced by High Concentration of Inorganic Phosphate and Cations

Comparison of High-Performance Ion Chromatography and absorptiometric methods for the determination of phytic acid in food samples

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