“…For example, Ca deficiency in laying ducks reduces the egg production rate, egg weight, eggshell thickness, eggshell breaking strength, and Ca deposition in the eggshell as a result of decreased Ca levels in the plasma and shell gland, as well as downregulating the expression of genes related to Ca transportation and eggshell biomineralization [ 33 , 34 ]. In White Pekin and Mule ducks, Ca deficiency can lead to rickets, low growth rate, low feed utilization, and a high mortality rate [ 35 , 36 ]. Xia et al [ 37 ] estimated the Ca requirements of Longyan laying ducks, from 20 to 33 wk of age, and recommended formulating diets containing 3.6% Ca with 0.40% available P to maximize egg production, egg mass, feed utilization, and enhance the bone characteristics.…”