Studies on Epidemic Diarrhea of the New-born: Isolation of a Filtrable Agent Causing Diarrhea in Calves

Light, Jacob; Hodes, Horace L.

doi:10.2105/ajph.33.12.1451

Cited by 93 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After initial passage in calves, Light and Hodes were able to perform from 2 to 29 successive calf passages by intranasal inoculation using unfiltered or Seitz-filtered stool specimens. Subsequent studies by electron microscopy of a lyophilized fecal specimen from a calf used in the original study of Light and Hodes (65,66), revealed viral particles of similar size and morphology as rotavirus (46). Although attempts to reproduce the disease in a calf using the lyophilized specimen were unsuccessful, the ability of human rotavirus to cause diarrhea in calves was confirmed in a study by Mebus et al.…”

Section: P R O C E D U R Ementioning

confidence: 93%

See 1 more Smart Citation

Cell culture propagation of rotaviruses

Saif

Rosen

Kang

et al. 1988

Journal of Tissue Culture Methods

View full text Add to dashboard Cite

SUMMARY: Rotaviruses are ubiquitous intestinal pathogens that infect the young of many species. Their adaptation to cell culture has met with variable success depending on the strain and serogroup of virus. Successful cultivation of rotaviruses has been achieved through sequential use of animal passsage and kidney cell cultures. Roller tubes were beneficial for adaption of several strains as opposed to use of stationary cell cultures. Activation of rotaviruses with proteolytic enzymes has increased their adaptability to culture and is standard practice with many strains. Alternative methods for cultivation of rotavirus, particularly human strains, have involved use of reassortants between animal and human rotaviruses. Continuous cell lines for rotavirus passage include rhesus monkey kidney (MA-104 & LLC-MK2k African green monkey kidney {BSC-1 & CV-1t, and Madin-Darby bovine kidney. Fluorescent loci, cytopathic effects, and plaque assays are used to titrate infectious virus. Enzyme-linked immunosorbent assay, hemagglutination, dsRNA electropherotyping, and electron microscopy are used to detect rotavirus growth. Optimal conditions for the growth of two group A rotaviruses (OSU & NCDV) in MA-104 cells are described in this report.

show abstract

Section: P R O C E D U R Ementioning

confidence: 93%

“…Two of the earliest reports on what has been suspected as rotavirus gastroenteritis were published by Hodes in 1943 and1949 (65,66). In these studies, a filterable agent was isolated from four hospital diarrheal outbreaks among newborn infants.…”

Section: P R O C E D U R Ementioning

confidence: 99%

Cell culture propagation of rotaviruses

Saif

Rosen

Kang

et al. 1988

Journal of Tissue Culture Methods

View full text Add to dashboard Cite

show abstract

“…[137] Three decades later, preserved samples of the agent were shown to be rotavirus. [138] In the intervening years, a virus in mice [139] was shown to be related to the virus causing scours.…”

Section: Historymentioning

confidence: 99%

Rotavirus

Beards¹

2017

Wiki J Med

View full text Add to dashboard Cite

Rotavirus is the most common cause of diarrhoeal disease among infants and young children. [1] It is a genus of double-stranded RNA viruses in the family Reoviridae. Nearly every child in the world is infected with rotavirus at least once by the age of five. [2] Immunity develops with each infection, so subsequent infections are less severe; adults are rarely affected. [3] There are eight species of this virus, referred to as A, B, C, D, E, F, G and H. Rotavirus A, the most common species, causes more than 90% of rotavirus infections in humans.The virus is transmitted by the faecal-oral route. It infects and damages the cells that line the small intestine and causes gastroenteritis (which is often called "stomach flu" despite having no relation to influenza). Although rotavirus was discovered in 1973 by Ruth Bishop and her colleagues by electron micrograph images [4] and accounts for approximately one third of hospitalisations for severe diarrhoea in infants and children, [5] its importance has historically been underestimated within the public health community, particularly in developing countries. [6] In addition to its impact on human health, rotavirus also infects animals, and is a pathogen of livestock. [7] Rotavirus is usually an easily managed disease of childhood, but in 2013, rotavirus caused 37 percent of deaths of children from diarrhoea and 215,000 deaths worldwide, [8] and almost two million more become severely ill. [6] Most of these deaths occurred in developing countries. [9] In the United States, before initiation of the rotavirus vaccination programme, rotavirus caused about 2.7 million cases of severe gastroenteritis in children, almost 60,000 hospitalisations, and around 37 deaths each year. [10] Following rotavirus vaccine introduction in the United States, hospitalisation rates have fallen significantly. [11][12] Public health campaigns to combat rotavirus focus on providing oral rehydration therapy for infected children and vaccination to prevent the disease. [13] The incidence and severity of rotavirus infections has declined significantly in countries that have added rotavirus vaccine to their routine childhood immunisation policies. [14][15] [16] Virology Types of rotavirusThere are eight species of rotavirus, referred to as groups A, B, C, D, E, F, G, and H. [17] Humans are primarily infected by species A, B and C, most commonly by species A. A-E species cause disease in other animals, [18] species E and H in pigs, and D, F and G in birds.[19] [20] Within rotavirus A there are different strains, called serotypes.[21] As with influenza virus, a dual classification system is used based on two proteins on the surface of the virus. The glycoprotein VP7 defines the G serotypes and the protease-sensitive protein VP4 defines P serotypes.[22] Because the two genes that determine G-types and P-types can be passed on separately to progeny viruses, different combinations are found.[22] A whole genome genotyping system has been established for group A rotaviruses, which has been used to ...

show abstract

“…In 1943 we reported our work briefly in the Bulletin of Hygiene (9). S o far as I know, this is the first report of the isolation of a virus which causes diarrhea in human beings.…”

mentioning

confidence: 93%

American Pediatric Society Presidential Address

Hodes

1976

Pediatr Res

View full text Add to dashboard Cite

Studies on Epidemic Diarrhea of the New-born: Isolation of a Filtrable Agent Causing Diarrhea in Calves

Cited by 93 publications

References 0 publications

Cell culture propagation of rotaviruses

Cell culture propagation of rotaviruses

Rotavirus

American Pediatric Society Presidential Address

Contact Info

Product

Resources

About