Studies on epizootic microsporidiosis in wild dusky grouper(Epinephelus guaza) from Mediterranean sea at Matrouh Governorate, Egypt

Abstract

ABSTRACThis study was carried out on 476 wild marine Dusky Grouper (Epinephelus guaza), captured from the West North shore of Mediterranean Sea at Matrouh Governorate from Sept. 2005 to Sept. 2006. The total prevalence of microsporidia among wild Epinephelus guaza fish was found to be 301 (63.23 %). The investigated fish, revealed the presence of cyst-like nodules ( xenomas, 2-4 mm. in diameter) in the gill arches. Each gill arch was infected with (1-5) xenomas and up to 30 nodules per fish. The recovered spores of Glugea anomala appeared as thick walled, uni-nucleated, pear-oval in shape with large vacuole. They measured (3-4 µm) in width and (5-6 µm) in length. The highest prevalence (82.9%) was recorded in summer, while the lowest one (30.33%) was recorded in winter. Presently, the highest infection rate (88.8%) with Glugea anomala was recorded among fish with 200-250g body weight, while the lowest one (43.3%) was recorded among fish of 100-150g body weight. The main histopathological alteration in gills was destruction of gill filaments with degenerative changes and infiltration of mononuclear leukocytes. The water parameters revealed the highest pH (7.3), chloride value (240 mg/L) and the total hardness (290 mg/L) in summer, while the lowest pH (5.7) and the total hardness (217mg/L) were recorded in winter, but the lowest chloride value (122 mg/L) was recorded in autumn.

Molecular characterization of Glugea anomala infecting Nile tilapia, Oreochromis niloticus and African Catfish, Clarias gariepinus and its associated pathological changes

“…Our finding could be attributed to the water temperature which is considered an important factor in development of microsporidia in different species of fish (Sveen et al, 2012). Additionally, it could explain the increase in the microsporidian infection rate in spring that fish may be eat excess infected zooplankton (Marzouk et al, 2010) . Histopathological studies are one of principal tools for the diagnosis of microsporidiosis (Sanders et al, 2020).…”

Ultrastructural and molecular characterization of Glugea serranus n. sp., a microsporidian infecting the blacktail comber, Serranus atricauda (Teleostei: Serranidae), in the Madeira Archipelago (Portugal)

“…Presently, there are 33 Glugea species, predominantly reported adhering to organs of the digestive tract of freshwater and marine fishes (Lom 2002;Vagelli et al 2005;Wu et al 2005;Voronin and Iukhimenko 2010;Su et al 2014;AbdelBaki et al 2015a, b;Azevedo et al 2016). To our best knowledge, and despite fish microsporidia lacking clear host specificity (Lom and Dyková 1992;Lom et al 1995;Nagasawa and Cruz-Lacierda 2004;Payghan et al 2009), the parasite here described constitutes the first occurrence of a Glugea species infecting a marine fish of the family Serranidae in the Madeira Archipelago, and one of the few reports of microsporidians infecting serranids worldwide (Zhang et al 2004(Zhang et al , 2005Wu et al 2005;Marzouk et al 2010;Jithendran et al 2011).…”

Protozoan Parasitic in Eels (Anguilla spp.) from Lindu Lake, Central Sulawesi

“…pada ikan sidat asal Danau Lindu sebesar 46 dan Marzouk et al (2010) prevalensi pada ikan kerapu tertinggi mencapai 88,8. Dampak yang ditimbulkan parasit ini seperti terbentuknya nodul berwarna coklat kehitaman pada jaringan adiposa dan organ visceral terutama pada dinding usus dan caecae pyloric (Jithendram et al 2011).…”