The daily course of leaf gas exchange was investigated on six linden cultivars from Tilia cordata Mill., T. platyphyllos Scop., T. tomentosa Moench and T. americana L. After the morning peak rapid stomatal closure was detected on leaves of T. cordata 'Savaria', 'Greenspire' and T. platyphyllos 'Favorit'. The stomatal conductance on leaves of T. tomentosa 'Szeleste' after the midday drop was significantly higher, while highest was on T. americana 'Redmond' leaves during the whole day. The transpiration resulted significant differences in daily water use (T. cordata 'Savaria' and T. platyphyllos 'Favorit': 1.0 kg•m −2 ; T. americana 'Redmond', 2.3 kg•m −2 of leaf area). Due to rapid stomatal closure T. cordata and T. platyphyllos cultivars realized water saving on the cost of strongly reduced carbon gain. Corresponding to their drought and heat adaptability the performance of leaves of T. tomentosa cultivars showed low level stress symptoms, intermediate level of transpiration and carbon gain. T. americana 'Redmond' leaves transpired largest water quantity, which resulted in large water loss and relatively intense stress symptoms. The large cultivar differences in the performance of leaf gas exchange should be considered at evaluation of drought stress adaptability and environmental benefits (CO 2 fixation, O 2 and vapor release) of Tilia cultivars.