Studies on Genetic Organization in Higher Organisms, II. Complementation and Fine Structure of the Maroon-like Locus of
            <i>Drosophila melanogaster</i>

Finnerty, Victoria; Duck, P.; Chovnick, Arthur

doi:10.1073/pnas.65.4.939

Cited by 39 publications

(19 citation statements)

References 5 publications

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“…In the latter case, a random strand analysis of recombination between maroon-like cistron mutants revealed that approximately half of the ma-l + exceptions exhibited exchange for the flanking markers. Utilizing the flanking marker recombinants, a unique internally consistent, linear, map of the ma-1 cistron was constructed which corresponds precisely with a complementation map (Finnerty, Duck & Chovnick, 1970). However, a half-tetrad analysis questioning the reciprocality of recombination events in heterozygotes for the most distant ma-1 alleles revealed that all of the events are non-reciprocal (Smith et al 1970a).…”

Section: Discussionmentioning

confidence: 99%

“…In higher eukaryotes, such as Drosophila melanogaster, observations which may be inferred to reflect conversion events have been reported on more than several occasions (Baillie, Astell & Scholefield, 1966;Chovnick, 1958Chovnick, , 1961Chovnick, Lefkowitz & McQuinn, 1956;Finnerty, Duck& Chovnick, 1970;Green, 1960;Hexter, 1963;Welshons & von Halle, 1962). Rigorous confirmation of these events as conversions has awaited the development of appropriate genetic systems for systematic investigation.…”

Section: Introductionmentioning

confidence: 93%

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Gene conversion in higher organisms: Non-reciprocal recombination events at the rosy cistron inDrosophila melanogaster

Ballantyne

Chovnick

1971

Genet. Res.

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Analysis of a series of exceptional ry + half-tetrads, produced in mass matings involving rosy mutant heterozygous half-tetrads, provides rigorous demonstration of the occurrence of non-reciprocal as well as reciprocal recombination events within the rosy cistron of Drosophila melanogaster. Inferences about allele recombination drawn from this and other studies in Drosophila provide a strong argument that gene conversion occurs as a regular event in higher eukaryotes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Gene conversion in higher organisms: Non-reciprocal recombination events at the rosy cistron inDrosophila melanogaster

Ballantyne

Chovnick

1971

Genet. Res.

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“…The minimal molecular weight of a protein coded by the ma-l+ cistron [9] is about 15000. Intracistronic complementation [35] requires the product to be at least a dimer, meaning that the moiety in xanthine dehydrogenase coded by the ma-1 + locus should have a molecular weight of at least 30000.…”

Section: Hypothesesmentioning

confidence: 99%

“…Some of these alleles show intracistronic complementation, i.e., flies heterozygous for two different ma-1 alleles in trans position possess xanthine dehydrogenase activity [9].…”

mentioning

confidence: 99%

Aldehyde Oxidase and Xanthine Dehydrogenase from Wild‐Type Drosophila melanogaster and Immunologically Cross‐Reacting Material from ma‐1 Mutants

Andres

1976

European Journal of Biochemistry

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The pleiotropic effect of the mu-1 mutation on the enzymes xanthine dehydrogenase and aldehyde oxidase in Drosophila melanogaster can most readily be explained by assuming that the enzymes share a subunit or cofactor whose synthesis is controlled by the mu-1 locus. According to this hypothesis a protein or a tightly bound cofactor common to both enzymes should be inactive or missing in the corresponding immunologically cross-reacting material found in ma-1 flies. Three of the proteins involved were purified by immunoadsorption : xanthine dehydrogenase, xanthine dehydrogenase cross-reacting material and aldehyde oxidase.On gel filtration xanthine dehydrogenase and xanthine dehydrogenase cross-reacting material showed identical molecular weights of 300000 whereas 280000 was found for aldehyde oxidase. All three proteins appear to be dimers. Under dissociating conditions (polyacrylamide electrophoresis in the presence of sodium dodecyl sulfate) a single protein band with a mobility corresponding to one half the native molecular weight was found. There was no evidence of small polypeptide subunits. All three proteins contain FAD, molybdenum and iron, cofactors known to be present in related vertebrate enzymes.Thus the mu-1 locus apparently does not control the mere incorporation of a polypeptide subunit or one of the prosthetic groups necessary for activity into the enzymes.The several genes affecting the activity of xanthine dehydrogenase and aldehyde oxidase in Drosophila melanogaster have attracted considerable attention as a possible model system for gene regulation in higher organisms [1,2]. Structural genes for the two enzymes are located near, but not immediately adjacent to, each other on the third chromosome: Flies homozygous for the rosy (ry) mutation at positions 3 -52 contain no detectable xanthine dehydrogenase [3] ; this has recently been confirmed in a detailed genetic analysis of this chromosomal region [4]. Using similar methods [5] the locus aldox (3 -57) has been found to be a structural gene for aldehyde oxidase.Mutations at a third locus, ma-1 (1 -64) [6] affect the activity of both xanthine dehydrogenase [3] and aldehyde oxidase [ 7 ] : Flies hemizygous or homozygous for this mutant lack both activities but do contain material which crossreacts immunologically

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“…To date, mitotic conversion, or the recovery of non-reciprocal recombinant products, has been found in yeast and fungi (see Fogel & Mortimer, 1971). Non-reciprocal products of meiosis have been reported in three D. melanogaster loci: garnet (Chovnick, 1961;Hexter, 1963Hexter, , 1964, maroon-like (Finnerty, Duck & Chovniok, 1970) and rosy (Chovnick et al 1970;Ballantyne & Chovnick, 1971).…”

Section: (I) Double Reciprocal Recombinationmentioning

confidence: 96%

Non-reciprocal intragenic mitotic recombination inDrosophila melanogaster

Kelly

1974

Genet. Res.

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X-ray-induced mitotic recombination within the lozenge locus of Drosophila melanogaster was found to be non-reciprocal. Recombination was detected by the presence of faceted spots in a smooth (lozenge) eye. Markers were present so that if recombination were reciprocal, equal numbers of vermilion and apricot-vermilion-coloured faceted spots would be formed. Of 19 faceted spots found among more than 60000 eyes examined, 10 were vermilion, 7 variegated (some vermilion and some apricot-vermilion-coloured facets), and 2 probably variegated. None was uniformly apricot-vermilion in colour. Four mechanisms for the formation of faceted spots are discussed: double reciprocal recombination, misrepair or gene conversion before chromatid replication, misrepair or gene conversion after chromatid replication, and misrepair or gene conversion followed by chromosome loss. The evidence most strongly supports the hypothesis that all faceted spots were formed by either misrepair following X-irradiation or gene conversion and were originally vermilion in colour. Subsequent chromosome loss from these vermilion cells led to the production of variegated spots. Variegated spots were generally smaller than those uniformly vermilion in colour, indicating that chromosome loss may occur with greater frequency in older irradiated larvae.

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Studies on Genetic Organization in Higher Organisms, II. Complementation and Fine Structure of the Maroon-like Locus of Drosophila melanogaster

Cited by 39 publications

References 5 publications

Gene conversion in higher organisms: Non-reciprocal recombination events at the rosy cistron inDrosophila melanogaster

Gene conversion in higher organisms: Non-reciprocal recombination events at the rosy cistron inDrosophila melanogaster

Aldehyde Oxidase and Xanthine Dehydrogenase from Wild‐Type Drosophila melanogaster and Immunologically Cross‐Reacting Material from ma‐1 Mutants

Non-reciprocal intragenic mitotic recombination inDrosophila melanogaster

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