Studies on glycolysis in vitro: Role of glucose phosphorylation and phosphofructokinase activity on total velocity

Meléndez-Hevia, Enrique; Siverio, JoséM.; Pérez, JoséA.

doi:10.1016/0020-711x(84)90162-9

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…The kinetic design of glycolysis and glycogen metabolism agrees well with the aim of quick release of large amounts of glucose 1-phosphate; it has been demonstrated by several groups that the availability of phosphorylated glucose is the most critical variable in controlling glycolytic flux (see Helmreich and Cori, 1965;Rapoport et al, 1974;Aragon et al, 1980;Fell, 1984;Melendez-Hevia et al, 1984Torres et al, 1986Torres et al, , 1988. On the other hand, the regulatory mechanism that triggers the release of glucose 1-phosphate from glycogen has been shown to be extremely efficient and quick.…”

Section: Introductionmentioning

confidence: 62%

Optimization of molecular design in the evolution of metabolism: the glycogen molecule

Meléndez-Hevia

Waddell

Shelton

1993

Biochemical Journal

160

173

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The animal glycogen molecule has to be designed in accordance with its metabolic function as a very effective fuel store allowing quick release of large amounts of glucose. In addition, the design should account for a high capacity of glucose storage in the least possible space. We have studied the optimization of these variables by means of a mathematical model of the glycogen molecule. Our results demonstrate that the structure is optimized to maximize (a) the total glucose stored in the smallest possible volume, (b) the proportion of it that can be directly released by phosphorylase before any debranching occurs, and (c) the number of non-reducing ends (points of attack for phosphorylase), which maximizes the speed of fuel release. The optimization of these four variables is achieved with appropriate values for two key parameters in glycogen design: the degree of branching and the length of the chains. The optimal values of these two parameters are precisely those found in cellular glycogen.

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Section: Introductionmentioning

confidence: 62%

Optimization of molecular design in the evolution of metabolism: the glycogen molecule

Meléndez-Hevia

Waddell

Shelton

1993

Biochemical Journal

160

173

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Glycolysis activity in flight muscles of birds according to their physiological function. An experimental model in vitro to study aerobic and anaerobic glycolysis activity separately

2009

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An experimental system in vitro is presented to assess the activity of the entire glycolysis in tissue extracts, which allows determining aerobic and anaerobic glycolysis activities separately. Glycolysis activity has been measured in pectoral and supracoracoideus muscles of the homing pigeon and the domestic fowl. These muscles support different aspects of flight in the two birds and are representative models of the two kinds of basic movements, endurance and sprint. The results obtained showed that in type I red fibers (pigeon pectoral), glucose produced a high glycolytic activity, while it was a poor substrate for type IIb white fibers (fowl pectoral and the two supracoracoideus). White fibers, however, attained its maximum glycolytic activity with phosphorylated glucose as substrate. These results demonstrated the validity of the experimental system as a method for assaying the two kinds of glycolytic activity in tissues, and supply new information about the biochemical and physiological features of these types of fibers.

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Activities of l-lactate and glycerol phosphate production rates in vitro from glucose 6-phosphate in regenerating rat liver

Siverio

Torres

Meléndez-Hevia

1985

International Journal of Biochemistry

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Studies on glycolysis in vitro: Role of glucose phosphorylation and phosphofructokinase activity on total velocity

Cited by 7 publications

References 22 publications

Optimization of molecular design in the evolution of metabolism: the glycogen molecule

Optimization of molecular design in the evolution of metabolism: the glycogen molecule

Glycolysis activity in flight muscles of birds according to their physiological function. An experimental model in vitro to study aerobic and anaerobic glycolysis activity separately

Activities of l-lactate and glycerol phosphate production rates in vitro from glucose 6-phosphate in regenerating rat liver

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