Studies on Granulocyte Heterogeneity in Elasmobranchs

Fine‐structural study of leucocytes in the goldfish, Carassius auratus

“…However, a few investigators have described internal structures of eosinophil granules in carp (Suwa, 1977) and in the rays Raja clavata (Mainwaring & Rowley, 1985) and Pavoraja spinifera (Hine & Wain, 1987). In the present study, goldfish eosinophils had granules with crystalloid bodies, and stratified structures with a periodicity of 5 or 9nm were recognized in the rod-like crystalloid bodies.…”

“…Watanabe et al (1967) and Daems (1968) reported that parallel linear structures were present in the granules of human neutrophils. Similar bodies were also described as fibrillar structures in neutrophils of plaice, Pleuronectes platessa (Ferguson, 1976), tilapia, Oreochromis niloticus (Suzuki, 1986), eel, Anguilla anguilla (Kelenyi, 1972), dogfish, Scyliorhinus canicula (Mainwaring & Rowley, 1985) and sturgeon, Acipenser brevirostrum (Hine & Wain, 1988). In carp, Cyprinus carpio, a lamellar structure was observed in heterophil granules (Suwa, 1977).…”

Composition and ultrastructure of elasmobranch granulocytes. II. Rays (Rajiformes)

“…Also dogfish neutrophilic granulocytes have small spherical granules at the EM level, but similar cells were not observed here or by other workers studying rays (Zapata, 1981;Pica et uf., 1983;Honma et ul., 1984;Mainwaring & Rowley, 1985). The granules of Puvoruju eosinophilic granulocytes, like the neutrophilic granulocytes, are fine and elongated, corresponding to the early stages of type RA granules, but no other lineage with elongated granules was observed in epigonal organ or blood.…”

“…Furthermore, another form of eosinophilic granulocyte may exist, as Torpedo, Raja spp., Myliobatis, Dasvatis, and the dogfish Dalatias licha (Hine & Wain, 1987a) have rounded eosinophilic granulocytes, that are polymorphonuclear, except in Torpedo. The early type RA granules o f Pavoraja closely resemble the granules of ' eosinophils ' (Hyder e f af., 1983) or ' granulocytes ' (Fange & Mattisson, 1981) of Giiigfymostoma, and ' heterophils ' (Morillas, 1981) of Halaeluris, both sharks, and ' eosinophilic granulocytes ' (Pica et af., 1983) ' acidophils ' (Zapata,198 1) and ' mature G 1 eosinophils ' (Mainwaring & Rowley. 1985) of Torpedo and Raja.…”

Evaluation of the Blood Film

“…17,18 There is a considerable amount of confusion in the literature with regard to the naming of the various granulocytes found in the different species of elasmobranchs, partially because the granulocytes exhibit a marked variation in numbers and types between the species. [18][19][20][21][22] An attempt to standardize and simplify the nomenclature involving elasmobranch granulocytes has been offered using avian rather than mammalian terminology. 23 This system allows the granulocytes of elasmobranch fishes to be classified as heterophils, eosinophils, and basophils using the descriptive criteria for those cells in avian hematology.…”