Studies on Granuloma Inguinale. V. Isolation of Bacterium resembling Donovania granulomatis from the Faeces of a Patient with Granuloma Inguinale.

Goldberg, Julius

doi:10.1136/sti.38.2.99

Cited by 21 publications

(18 citation statements)

References 2 publications

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“…With the exception of the developing chick embryo (and other types of eggs) K. granulomatis is pathogenic only for humans (Dienst & Bronwell, 1984) where infection results in the chronic genital ulcerative disease known as donovanosis. K. granulomatis has been assumed to be sexually transmitted (Rajam & Rangiah, 1954;Sengupta, 1981;Spence, 198%;Sehgal & Sharma, 1992;Hart, 1997) though an enteric habitat has been postulated (DeMonbreun & Goodpasture, 1933 ;Dunham & Rake, 1948;Goldberg, 1962Goldberg, , 1964. Goldberg (1962) isolated and cultured K. granulomatis from the faeces of a patient with donovanosis and DeMonbreun & Goodpasture -( 1933) also claim to have achieved successful isolation (though not culture) of K. granulornatis from the faeces of 2/4 patients with donovanosis.…”

Section: Cell Characteristicsmentioning

confidence: 99%

Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov.

Carter

Bowden

Bastian

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

108

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By sequencing a total of 2089 bp of the 16s rRNA and phoE genes it was demonstrated that Calymmatobacterium grandomatis (the causative organism of donovanosis) shows a high level of identity with Klebsiella species pathogenic to humans (Klebsiella pneumoniae, Klebsiella rhinoscleromatis). It is proposed that C. grandomatis should be reclassified as Klebsiella granulomatis comb. nov. An emended description of the genus Klebsiella is given.

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Section: Cell Characteristicsmentioning

confidence: 99%

Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov.

Carter

Bowden

Bastian

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

108

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“…It is suggested that the pleomorphic forms are undergoing a process of bacterial elongation and active replication. The pleomorphic nature of the organism in culture has been described by several workers [9,16,201. The bacteria in the monocyte coculture after incubation for 48 h at 37°C in CO2 5% in air were extremely pleomorphic, varying in size and shape in comparison to those usually seen in direct smears, suggesting that these organisms were in a process of cell division and multiplication.…”

Section: Discussionmentioning

confidence: 94%

Growth and cultural characteristics of Calymmatobacterium granulomatis - the aetiological agent of granuloma inguinale (Donovanosis)

Kharsany

Hoosen²,

Kiepiela³

et al. 1997

Journal of Medical Microbiology

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Granuloma inguinale is a chronic destructive granulomatous disease of the genitalia. The clinical diagnosis is often unreliable and the definitive diagnosis is based on the visualisation of 'Donovan bodies' in tissue smears or biopsy specimens. The organism implicated in its aetiology, Calymmatobacterium granulomatis, was reported to have been cultured >30 years ago, but little is known about the organism because of its fastidious nature and the difficulty in culturing it. Twenty-two biopsy specimens from female patients with clinical and laboratory-confirmed granuloma inguinale were treated with amikacin 10 mg/L and inoculated in a monocyte co-culture system with peripheral blood mononuclear cells (PBMC) from a single donor and autologous sera. The method was subsequently modified by pretreatment of specimens with vancomycin 5 mg/L and metronidazole 10 mg/L in addition to amikacin 10 mg/L for the purpose of decontamination, pooled blood donor PBMC and by the use of heat-inactivated fetal calf serum instead of autologous serum for culture. This modified method was used to culture additional biopsy specimens and genital ulcer scrapings from female and male patients, respectively. All monocyte co-cultures were examined by a rapid Giemsa (RapiDiff) stain and by an indirect immunofluorescence test with immune sera. Representative cultures were examined by transmission electron microscopy. C. granulomatis was successfully isolated in pure culture by the monocyte co-culture system from four biopsy specimens and 14 genital ulcer scrapings. The cultured organisms were visible both intra-and extra-cellularly and were extremely pleomorphic, with characteristic single and bipolar condensation. The numbers of the organisms increased after each passage. All positive cultures showed bright fluorescence when tested with immune sera. Transmission electron microscopy of the cultured bacteria demonstrated a typical gram-negative cell wall consisting of an outer membrane, middle electron opaque layer and an inner plasma membrane. The capsule was thick and electron dense. Numerous electron dense granules were present within the cytoplasm.

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“…37 It has been demonstrated in fecal flora as a coccobacillus measuring 0.5-1.5 m wide and 1.0 m long, with round extremities. 38 The coccobacilli have a polysaccharide and fibrous capsule. C granulomatis demonstrates a chromatin condensation in one or both extremities, forming characteristic shapes in ''halters'' or '' safety pins'' when stained with Giemsa or Wright stains.…”

Section: Granuloma Inguinalementioning

confidence: 99%

Tropical dermatology: Bacterial tropical diseases

Lupi

Madkan

Tyring

2006

Journal of the American Academy of Dermatology

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Studies on Granuloma Inguinale. V. Isolation of Bacterium resembling Donovania granulomatis from the Faeces of a Patient with Granuloma Inguinale.

Cited by 21 publications

References 2 publications

Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov.

Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov.

Growth and cultural characteristics of Calymmatobacterium granulomatis - the aetiological agent of granuloma inguinale (Donovanosis)

Tropical dermatology: Bacterial tropical diseases

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