Studies on haptoglobin binding to concanavalin a

Kaţnik, I

doi:10.1016/0167-4838(84)90325-x

Cited by 14 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Haptoglobin is heavily glycosylated resulting in a net negative charge [32, 33]. Because of its large size and negative charge, the haptoglobin-hemoglobin complex is not filtered at the glomerulus.…”

Section: Discussionmentioning

confidence: 99%

Urine haptoglobin levels predict early renal functional decline in patients with type 2 diabetes

Bhensdadia

Hunt

Lopes‐Virella

et al. 2013

Kidney International

View full text Add to dashboard Cite

Diabetic nephropathy is the leading cause of end stage renal disease. The urinary albumin to creatinine ratio is used as a predictor for the development of nephropathy but it is neither sensitive nor specific. Here we used liquid chromatography/mass spectrometry on urine of eight normoalbuminuric patients with type 2 diabetes from the VA Diabetes Trial to identify candidate markers for loss of renal function. Initial verification of 7 markers (agrin, haptoglobin, mannan-binding lectin serine protease 2, LAMP-2, angiotensinogen, NGAL and uromodulin) in the urine of an additional 30 patients showed that haptoglobin was the best predictor of early renal functional decline. We then measured this in the urine of 204 patients with type 2 diabetes who did not yet have significant kidney disease (eGFR stage 2 or better and an albumin to creatinine ratio less than 300 mg/g). In comparing the highest to lowest tertile, the odds ratio for having early renal function decline was 2.70 (CI 1.15, 6.32) using the haptoglobin to creatinine ratio compared to 2.50 (CI 1.14, 5.48) using the albumin to creatinine ratio after adjusting for treatment group and use of ACE inhibitors. Addition of the haptoglobin to creatinine ratio to a model using the albumin to creatinine ratio to predict early renal function decline resulted in improved predictive performance. Thus, the haptoglobin to creatinine ratio may be useful to predict patients with type 2 diabetes at risk of nephropathy prior to the development of macroalbuminuria or reduced GFR.

show abstract

Section: Discussionmentioning

confidence: 99%

Urine haptoglobin levels predict early renal functional decline in patients with type 2 diabetes

Bhensdadia

Hunt

Lopes‐Virella

et al. 2013

Kidney International

View full text Add to dashboard Cite

show abstract

“…For instance Hp-Hb and Hp-concanavalin A concentrations in a patient with ovarian cancer were extremely high: 6.7 g/1 and 7.3 g/1, respectively, hence the It is well documented that quantitative changes in the concentrations of acute phase proteins during certain pathophysiological states, particularly in inflammatory conditions and cancer, often accompany specific alterations of the carbohydrate moieties of glycoproteins. Changes in the degree of branching (major microheterogeneity) or/and in terminal glycosylation of glycoproteins (minor microheterogeneity) originate from modifications in the post-translational glycosylation mechanism in the liver or from degradative processes in the circulation (4,5,15,16).…”

Section: Resultsmentioning

confidence: 99%

Measurements of Haptoglobin by the Reaction with Concanavalin A in Sera of Patients with Ovarian Tumours

Kaţnik¹,

Jadach²,

Kmieciak³

et al. 1995

CCLM

View full text Add to dashboard Cite

Summary:The concentration of haptoglobin in sera of healthy women and patients with non-malignant and malignant ovarian tumours was measured by two methods, i.e. complex formation with haemoglobin and complex formation with concanavalin A. High correlation (r = 0.74) between both the methods was found in the group of healthy women, but correlation coefficients were much lower in the group of non-malignant and malignant tumours (r = 0.42 and 0.37, respectively). Direct determinations of haptoglobin, and calculation of the ratio of haptoglobin bound to concanavalin A to haptoglobin bound to haemoglobin revealed statistically significant differences among the examined groups. Comparison of two methods of haptoglobin quantitation suggest that processes connected with ovarian disorders may alter the glycosylation of haptoglobin oligosaccharide chains.

show abstract

“…Increase in sialic acid content reduces the binding towards concanavalin A (23). The concanavalin A non-binding fraction of haptoglobin does not form complexes with haemoglobin (24), so the haemoglobin-binding capacity of haptoglobin is re-duced during the early phase of acute myocardial infarction. Furthermore, recent findings postulate a role for haptoglobin in homing and trafficking of B lymphocytes (25).…”

Section: Discussionmentioning

confidence: 99%

Evolution of Haptoglobin Concentration in Serum during the Early Phase of Acute Myocardial Infarction

Bernard¹,

Langlois²,

Delanghe³

et al. 1997

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

is a haemoglobin-binding acute phase protein with three genetic types: Hp 1-1, Hp 2-1, Hp 2-2. We investigated 45 patients during the first 48 hours of acute myocardial infarction, and studied determinant factors and clinical correlates. Upon hospital admission, serum haptoglobin concentration was increased (1.95 ± 0.94 g/1, mean ± SD, P < 0.001) versus the reference population (0.97 ± 0.46 g/l, n = 107), independent of haptoglobin type: 1.84 ± 0.64 g/1 (Hp 1-1, n = 11) (P < 0.01), 1.98 ± 0.79 g/l (Hp 2-1, n = 25) (P < 0.001), 1.98 ± 1.58 g/l (Hp 2-2, n = 9) (P < 0.001). Moreover, during the first hours of hospitalization, a temporal lowering of haptoglobin was observed suggesting acute haemolysis, independent of the haptoglobin type. Minimal serum haptoglobin was reached 9.6 ± 5.8 hours after admission. The amplitude of the haptoglobin decrease correlated with initial serum haptoglobin (r = 0.78) and was more pronounced (P < 0.05) in men (0.53 ± 0.57 g/1) than in women (0.18 ± 0.17 g/1). Decrease of serum haptoglobin did not correlate with infarct size (based on creatine kinase-MB release). Out of the other acute phase proteins measured upon admission, only C-reactive protein was significantly increased (P < 0.05). During the next 36 hours, haptoglobin increased as a result of the acute phase response to myocardial injury. Our findings suggest that acute myocardial infarction is also preceded by an acute phase response, characterized by an initial high haptoglobin and followed by a temporal haptoglobin decrease due to haemolysis.

show abstract

Studies on haptoglobin binding to concanavalin a

Cited by 14 publications

References 20 publications

Urine haptoglobin levels predict early renal functional decline in patients with type 2 diabetes

Urine haptoglobin levels predict early renal functional decline in patients with type 2 diabetes

Measurements of Haptoglobin by the Reaction with Concanavalin A in Sera of Patients with Ovarian Tumours

Evolution of Haptoglobin Concentration in Serum during the Early Phase of Acute Myocardial Infarction

Contact Info

Product

Resources

About