Studies on Heparin and the Lipemia Clearing Factor. I

Hood, B.; Angerwall, G.; Isaksson, B; Welin, Gunnar

doi:10.1080/00365515409134820

Cited by 8 publications

(5 citation statements)

References 4 publications

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“…A reduced absorption or utilization of fats and other food calories probably accounts for the suppression of PPLA levels in patients with malabsorption syndrome, 29 in patients with deficient beta-lipoprotein formation, and in those with severe grades of dietary fat restriction. 10 …”

Section: Discussionmentioning

confidence: 99%

Lipolytic Activity of Post-Heparin Plasma in Hyperlipemia and Hypolipemia

Kuo

Bassett

DiGeorge

et al. 1965

Circulation Research

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(1) The role of post-heparin plasma lipolytic activity (PPLA) in the production of hyperglyceridemia was investigated in patients with high concentrations of plasma triglycerides produced in two ways, viz. fat-induced and "mixed" (both fat and carbohydrate-induced). While on a self-chosen diet, the PPLA levels of patients with "mixed" type of hyperglyceridemia differed little from those of young healthy subjects. PPLA levels of these patients were promptly lowered by rice diet, despite the persistence of hyperglyceridemia. Normal serum lipid concentrations and low PPLA levels were induced and maintained in these patients by reducing moderately their intake of both dietary fat and calories. (2) PPLA levels were reduced in the fat-induced hyperglyceridemic patients both before and after the use of low fat diet to reduce their plasma triglyceride concentrations. (3) PPLA was also low in patients with low and absent beta-lipoproteins, and in those with disturbance of intestinal fat digestion and absorption.

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Section: Discussionmentioning

confidence: 99%

Lipolytic Activity of Post-Heparin Plasma in Hyperlipemia and Hypolipemia

Kuo

Bassett

DiGeorge

et al. 1965

Circulation Research

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“…been intensively studied by many investigators as related to atherosclerosis. Some (12)(13)(14)(15)(16)(17) observed lower LPL activity in patients with atherosclerosis, but others (8,(18)(19)(20)(21)(22) found no difference in LPL activity between normal and atherosclerotic subjects. Our results showed that the peak values were the same in both groups.…”

Section: Resultsmentioning

confidence: 99%

Kinetic Studies on Metabolism of Lipoprotein Lipase*

Yoshitoshi¹,

Naito²,

Okaniwa³

et al. 1963

J. Clin. Invest.

106

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Since Hahn demonstrated the clearing of alimentary lipemia by the intravenous injection of heparin, many excellent studies have been done on lipoprotein lipase (LPL). In these studies, however, LPL activities were compared in normal and pathological conditions at a certain time after heparin injection. If the level of LPL activity in the circulating blood changes continuously after heparin injection, under the influences of production and inactivation of LPL, it may be much more useful, under certain circumnstances, to measure the rate of change as well as the amount of such activity.One of the authors reported previously (1) the method of estimating the release or production rate and inactivation rate of LPL after heparin injection. This report will present the data on the metabolism of LPL activity in blood after the intravenous injection of heparin in normal subjects and patients with liver diseases, obesity, and coronary sclerosis, and in some animals. EXPERIMENTAL METHODSPreparation of materials. Human subjects and experimental animals were fasted overnight before the experiment. Experimental animals (dogs and rabbits) were anesthetized with pentobarbital sodium (Nembutal) just before use.Heparin injection. The doses of heparin injected were 0.1 mg per kg of body weight throughout the experiment. The injection was done rapidly into the antecubital vein in men and dogs and into the ear vein in rabbits.Withdrawals of blood. At certain intervals after the injection, blood was withdrawn from the antecubital vein in human subj ects, from the femoral vein in dogs, and by heart puncture in rabbits. The blood specimen was immediately added to 1/9 vol of Y/10 M sodium oxalate solution kept in an ice-water bath.Assay of LPL activity. The test plasma weas obtained by centrifuging the blood for 5 minutes at 4,000 rpm in the cold. The substrate consisted of 0.1 vol of 2.5%o * Supported in part by a grant-in-aid for research from the Ministry of Education and a grant-in-aid for atherosclerosis study from the Ministry of \Velfare. Immediately after the mixing of 1 ml of test plasma with 1.5 ml of substrate, 1.0 ml of the mixture was pipetted into Dole's extraction mixture, and the remainder was incubated at 390 C for 30 minutes. The LPL activity was expressed by the difference of FFA concentrations (microequivalents per liter) in the mixture before and after the incubation. FFA was measured by Dole's method (2). The details of our method were discussed in the previous paper (1). RESULTS AND DISCUSSIONLPL activity curve in normnal human beings, dogs, and rabbits. Eight healthy men aged 25 to 35 years, 4 dogs, and 3 rabbits were used. Figures 1 and 2 show the changes of LPL activity as a function of time in human subjects and dogs, respectively. Similar changes of LPL activity were observed also in rabbits. The rapid rise in LPL activity was observed immediately after heparin injection, and the peak was after about 9 minutes in human subjects, about 8 minutes in dogs, and about 3 minutes in rabbits. Thereafter, the activity...

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“…8 ' 10 No difference was found with 100 mg. doses between normal and atherosclerotic subjects. 11 In elderly atherosclerotic patients used in this study, considerable lipolytic activity developed following each dose of heparin although most subjects had been receiving injections of heparin twice weekly for several years. Apparently heparin effectively clears lipemia EXGELBERG regardless of the number of previous injections.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Studies of Triglyceride Lipolysis after Heparin Administration

Engelberg

1958

Circulation Research

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The extent of triglyeeride lipolysis by postheparin lipemia clearing factor (lipoprotein lipase) was studied in vitro in 8 atherosclerotic individuals. Varying doses of heparin were injected intravenously and subcutaneously and blood was drawn at stated time intervals for analysis. The rate of splitting of human low density lipoproteins was then determined by measurement of the rate of release of unesterined fatty acids upon incubation of postheparin plasma plus lipoproteins in vitro at 37 C. The data obtained gives a range of the minimum in vivo removal of alimentary neutral fat following an injection of heparin.

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Studies on Heparin and the Lipemia Clearing Factor. I

Cited by 8 publications

References 4 publications

Lipolytic Activity of Post-Heparin Plasma in Hyperlipemia and Hypolipemia

Lipolytic Activity of Post-Heparin Plasma in Hyperlipemia and Hypolipemia

Kinetic Studies on Metabolism of Lipoprotein Lipase*

Quantitative Studies of Triglyceride Lipolysis after Heparin Administration

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