Studies on Heterophile Antigen in Lymphoma and Leukemia Spleens by Means of Absorption of Infectious Mononucleosis Sera

Milgrom, Felix; Kano, Kyoichi; Fjelde, Audrey

doi:10.1159/000231106

Cited by 14 publications

(12 citation statements)

References 3 publications

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“…The precipitin in IM sera was shown to be the P-B antibody and the precip itinogen in syphilis and leprosy sera was shown to be long to the P-B antigenic complex and to be closely related to the previously described B antigen of this complex [5], As mentioned above, in our previous investiga tions [1][2][3][4], we noted P-B antigen in human pathologi cal tissues and sera. In one study [3] we demonstrated the B antigen in 28% of lymphoma-leukemia sera test ed.…”

Section: Discussionsupporting

confidence: 53%

“…In spite of the expression of P-B antigens in spleens of patients with lymphoreticular malignan cies [1][2][3] as well as in some cancer tissues [4], no P-B antibody response has been observed in these patients [see the reviews, ref. [14][15][16][17], In our initial study by means of hemagglutination inhibition (HI) tests [4], P-B antigen was found in sera of 5-12% of these pa tients.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies have shown that Paul-Bun nell (P-B) antigens of infectious mononucleosis (IM) are expressed in spleen cells and spleen tissue extracts of many patients with Hodgkin's disease, lymphomas or leukemias [1][2][3] and that these antigens are also de tectable in sera of patients with cancer, systemic lupus erythematosus (SLE), rheumatoid arthritis or lepromatous leprosy [4], Most of these studies were per formed by a very sensitive procedure in which agglu tination of bovine (BRBC) or sheep erythrocytes (SRBC) by IM serum was abolished by absorption or neutralization of P-B antibodies with the tested speci men. More recently, two distinct antigens, BS and B of the P-B antigenic complexes, were defined serolog ically in extracts of bovine erythrocyte stromata [5] and they were also demonstrated in extracts of some pathological human tissues [3], In continuing this line of investigation in the present study, sera of syphilis patients at various stages of the disease and sera of pa tients with lepromatous leprosy were examined against selected IM sera by means of double-diffusion precipitation tests in agarose gel.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, two distinct antigens, BS and B of the P-B antigenic complexes, were defined serolog ically in extracts of bovine erythrocyte stromata [5] and they were also demonstrated in extracts of some pathological human tissues [3], In continuing this line of investigation in the present study, sera of syphilis patients at various stages of the disease and sera of pa tients with lepromatous leprosy were examined against selected IM sera by means of double-diffusion precipitation tests in agarose gel. 1 Supported by research grant CA-27628 from the National Can cer Institute and by the Richard W. and Mae Stone Goode Estate.…”

Section: Introductionmentioning

confidence: 99%

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Studies on the Paul-Bunnell Antigen-Antibody System

Morito¹,

Kano²,

Milgrom³

1983

Int Arch Allergy Immunol

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By means of double-diffusion precipitation tests in agarose gel with selected infectious mononucleosis (IM) sera, an antigen was detected in sera of patients with syphilis and leprosy. Of 378 syphilis sera tested 39 (10.3%) and of 36 leprosy sera 7 (19.4%) gave positive results. Sera of patients with various other diseases were negative. This antigen was demonstrable in sera of patients with both early and late syphilis and was shown to be unrelated to cardiolipin. Absorption studies of the IM sera with bovine and sheep erythrocytes and guinea pig tissues revealed that this antigen was identical with or closely related to the previously described B antigen of the Paul-Bunnell (P-B) antigenic complex and distinct from heterophile antigens of Hanganutziu-Deicher (H-D) and of Forssman specificity. However, 2 of 89 syphilis sera without the P-B antigen were shown to contain antigen(s) of H-D specificity. None of the syphilis or the leprosy sera contained P-B antibodies, but H-D antibodies were found in 13% of syphilis sera.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Studies on the Paul-Bunnell Antigen-Antibody System

Morito¹,

Kano²,

Milgrom³

1983

Int Arch Allergy Immunol

Self Cite

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“…In this connection, it is note worthy that PB antigen appears in mice as a T cell marker [7. 14] and that the spleen of some patients with lymphoma or leukemia contain this antigen [15]. Thus far, the anti gen has not been demonstrated on atypical lymphocytes in peripheral blood of IM pa tients [16],…”

Section: Discussionmentioning

confidence: 99%

Immune Deposit Nephritis in Infectious Mononucleosis

Andres

Kano

Elwood

et al. 1976

Int Arch Allergy Immunol

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A 22-year-old white male (L. V.) died of gram-negative septicemia complicacating infectious mononucleosis (IM) that was associated with jaundice and oliguric renal failure. The kidney showed mesangial granular deposits of IgM and C₃, mesangial electron-dense deposits, and interstitial infiltrates of mononuclear cells, including atypical lymphocytes. Eluates obtained from kidney, spleen and liver contained Paul-Bunnell (PB) antibodies. Presence of PB antigens in these tissues was indicated by absorption of PB antibodies from IM sera, with the sediments resulting from tissue elutions. The IgM mesangial deposits were partially eluted with acid buffer at 56 C and then reconstituted by incubation with IM sera or with immunoglobulins eluted from tissues of patient L. V. The presence in renal structures of PB antigens, IgM heterophile antibody, C₃ and electron-dense deposits is consistent with the hypothesis that heterophile immune complexes were localized in the kidney and that they contribute in the pathogenesis of IM nephritis.

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Isolation and partial characterization of the heterophile antigen of infectious mononucleosis from bovine erythrocytes

Merrick¹,

Schifferle²,

Zadarlik³

et al. 1977

J Supramol Struct

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The heterophile antigen (Paul-Bunnell antigen, PBA) of infectious mononucleosis was isolated by extraction of an aqueous suspension of bovine erythrocyte stromata with chloroform-methanol (2:1). The upper aqueous layer contained gangliosides, PBA, and a high-molecular-weight glycoprotein. PBA and gangliosides were separated from the high-molecular-weight glycoprotein by extraction of lyophilized upper layer with chloroform-methanol solvents. Separation of PBA from gangliosides was carried out by chromatography on DEAE-cellulose with chloroform-methanol solvents. PBA appeared to be a minor glycoprotein component of the erythrocyte membrane and had both hydrophobic and hydrophilic properties. It was soluble in either organic or aqueous solvents. On SDS-polyacrylamide gel electrophoresis, it migrated as a single component that stained for protein with Coomassie blue, for carbohydrate with periodic acid-Schiff reagent, and for lipid with oil red 0; it had an apparent molecular weight of 26,000. It was composed of 62% protein with major amino acids; glutamic acid, proline, glycine, isoleucine, leucine, and threonine (158, 116, 98, 90, 85, and 82 residues per 1,000 residues, respectively). Carbohydrate content was 9.2% with major sugar constituents: sialic acid, galactosamine, and galactose. Serologic activity of PBA was destroyed by pronase but not by trypsin.

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Studies on Heterophile Antigen in Lymphoma and Leukemia Spleens by Means of Absorption of Infectious Mononucleosis Sera

Cited by 14 publications

References 3 publications

Studies on the Paul-Bunnell Antigen-Antibody System

Studies on the Paul-Bunnell Antigen-Antibody System

Immune Deposit Nephritis in Infectious Mononucleosis

Isolation and partial characterization of the heterophile antigen of infectious mononucleosis from bovine erythrocytes

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