Studies on Human Gastric Intrinsic Factor

Studies on Human Gastric Intrinsic Factor

“…Many investigations showed that immunodiffusion and immunoelectrophoresis combined with autoradiography were useful for testing human and hog IF (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). In the present investigation, the purified IF materials, obtained from normal human gastric juice (NHGJ) as described in the previous paper (13), were further analysed by immunodiffusion and immuno electrophoresis techniques combined with autoradiography, using the rabbit antisera obtained after successive injections of the purified materials with Freund's complete adjuvant in order to evaluate their purities. …”

Studies on Hog Intrinsic Factor

“…Although the autoantibodies were not examined in the patients tested, cross reaction between hog IF and rabbit anti-human IF antiserum was not found on radioimmunodiffu sion (25). Therefore, the negative result in patients with pernicious anemia seems to have no relation to the autoantibodies.…”

Studies on Human Gastric Intrinsic Factor

“…More recently, immunological assay has been used for the determination of IF in human gastric juice (16)(17)(18)(19)(20)(21). In the present experiments , the in vitro assay using everted sacs of guinea pig intestine was employed in order to ascertain whether purified human IF materials described in the previous paper (22) possessed the activity and physicochemical properties of IF or not. The IF activity in gastric juices from patients having histamine-fast anacidity with and without PA were also assayed by this method.…”

“…Protein concentration was calculated by the method of Warburg and Christian (24, 25) as described previously (22). B12 binding capacity of the pooled NHGJ was determined by dialysis technique (26).…”