Studies on immunopotentiating activities of antitumor polysaccharide from aerial parts ofTaraxacum platycarpum

Jeong, Jong Yup; Chung, Yeoun Bong; Lee, Chong Chull; Park, Soo Wan; Lee, Chung Kyu

doi:10.1007/bf02857817

Cited by 9 publications

References 12 publications

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Water-soluble polysaccharide from Taraxacum platycarpum: isolation, chemical compositions, and antioxidant activity

et al. 2012

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Taraxacum platycarpum has been used as à traditional Chinese medicine (TCM) for more than a thousand years [1]. Recent pharmacological investigations show that the phenolic acids, triterpenes, phytosterols, flavonoids, and polysaccharides of T. platycarpum possessed several proven pharmacologic activities [2][3][4]. We focused on the elucidation, isolation, and chemical and physical characteristics of polysaccharides from T. platycarpum and antioxidant effects in vitro.The yield of the crude polysaccharide (CTPP) was 9.54%. The main fraction (TPP-a) was purified on DEAE-cellulose and Sephacryl S-200 columns to yield 38.6% of the crude polysaccharide. The total sugar, protein, uronic acid contents, molecular weight, and monosaccharide composition of the polysaccharide fraction are summarized in Table 1. The carbohydrate content of TPP-a was 96.3%. TPP-a gave a negative response to the Bradford test and no absorption was detected at 280 and 260 nm, indicating the absence of protein and nucleic acid. TPP-a was composed of arabinose, mannose, galactose, and glucose in molar ratios of 2.1:1:1.5:4.8. The HPGPC profile demonstrated that TPP-a was a homogeneous polysaccharide, and its molecular weight was estimated as 58.6 kDa. The IR spectrum of TPP-a revealed a typical major broad stretching peak at 3409 cm -1 for the hydroxyl group, and the weak band at 2926 cm -1 was attributed to C-H stretching vibrations. The band at 847 cm -1 and 885 cm -1 indicated D-and E-configurations of the sugar units simultaneously existing in the polysaccharide. Figure 1 demonstrates the DPPH scavenging activity caused by different concentrations of CTPP and TPP-a. The DPPH radical scavenging activity of CTPP and TPP-a reached 52.9% and 76.5% at 8 mg/mL, respectively. The DPPH scavenging activity of TPP-a was significantly higher (P < 0.01) than that of CTPP. The scavenging activity increased steadily at concentrations of 0.05-8 mg/mL for CTPP and TPP-a. 0 1 2 3 4 5 6 7 8 0 20 40 60 80 1 2 Scavenging rate of DPPH radical, % Concentration, mg/mL Fig. 1. Scavenging rate of polysaccharide from T. platycarpum against DPPH radical: CTPP (1), TTP-a (2). Results are presented as means ±S.D. (n = 3).

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