Studies on Incidence and Prevention of Nosocomial Infection of Urinary Tract Endoscopies by Different Antimicrobial Agents

Abstract

Antibiotic susceptibility profile of purified bacteria revealed four multi-resistant strains identified as Staphylococcus aureus Zag11, Pseudomonas aeruginosa Zag60, Escherichia coli Zag126 and Staphylococcus epidermidis Zag128. The four selected bacteria were subjected to some disinfectants (glutaraldehyde, hydrogen peroxide, P3-oxonia and Orthophthaladehyde) at different concentrations and different exposure times. It was observed that 10 min were enough to inhibit growth of tested pathogenic bacteria in case of (8% H 2 O 2 & 0.55% orthophthaladehyde) while completely inhibition was recorded after 15min in case of (2.2% glutaraldehyde, 70% ethanol and 0.45% P3-oxonia). Sterile urinary tract endoscopy was artificially contaminated with mixture (1:1:1:1:1:1) of six clinical pathogenic bacterial strains comparing with the four tested bacterial strains. Upon exposing the contaminated endoscope to different chemical disinfectants; 8% hydrogen peroxide and 0.55% Orthophthalaldehyde inhibited after 30 min exposure while 2.2% Glutaraldehyde, 0.45% P3oxonia and 70% Ethanol needed 60 min for complete bacterial inhibition. Upon exposure of artificially contaminated endoscope to different physical agents (U.V, γrays and dry hot air), Gamma rays showed maximum inhibitory action.