Studies on Isolation, Characterization and in-vitro Screening of Plant Growth Promoting Rhizobacteria from Rhizospheric Soil of Chrysanthemum (Dendranthema grandiflora Tzvelev.)

Ranadev, Praveen; Nagaraju, K.; Muthuraju, R.; Kumari, Renu

doi:10.20546/ijcmas.2019.806.096

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…We consider here crop plants whose roots are inhabited by introduced organisms, referring to them as Enhanced Plant Holobionts (EPHs, thus increasing the physiological integrity of the plant to grow optimally. The plant growth improvement can also be derived from the symbiotic relationship between PGPR (B. subtilis and P. flourescens) and the host plant (Ranadev et al, 2019). While so far, antagonist Cladosporium sp.…”

Section: Intensity Of Parasitismmentioning

confidence: 99%

Synergistic Action of PGPR and Biofungicide with Active Ingredient of Cladosporium clasporioides to Control White Rust on Chrysanthemum

Nuryani

Hanudin²,

Rahardjo³

et al. 2022

Agrivita.J.Agr.Sci

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The application of antagonist microbes to control significant diseases on crops is an essential issue in the eco-friendly and sustainable agriculture of the chrysanthemum production system. The application of antagonist consortiums is expected to synergistically suppress the pathogen more effectively than a single microbe, thus increasing the marketable flower yield. The research is carried out to evaluate the single and combined application of antagonists C. ladosporioides and PGPRs, B. subtilis, and P. flourescens to control white rust in Chrysanthemum. The results show that there is no cumulative effect from the combination of biofungicide and the PGPR on disease suppression, disease incidence, and plant growth improvement than single antagonist treatments. Compared to synthetic fungicide, biofungicide and the PGPR treatments give higher parasitism intensity, though the values were negligible among the treatments. The lowest disease intensity is recorded from synthetic fungicide treatment. The improvement of flower quality due to biofungicide, PGPR, and synthetic fungicide treatments was found only on the longer vase life than untreated plants. Observation on the compatibility of antagonists with PGPRs are still needed to increase the effectiveness in controlling white rust in Chrysanthemum.

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Section: Intensity Of Parasitismmentioning

confidence: 99%

Synergistic Action of PGPR and Biofungicide with Active Ingredient of Cladosporium clasporioides to Control White Rust on Chrysanthemum

Nuryani

Hanudin²,

Rahardjo³

et al. 2022

Agrivita.J.Agr.Sci

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“…Chrysanthemum (Dendranthema grandiflora Tzvelev) is the number one flower crop in many countries, including India, the United States, and Japan (Ranadev, Kumari and Praveen Ranadev, 2019). Dendranthema grandiflora is a synonym of Chrysanthemum morifolium Ramat (Lowe et al, 1993;Sjahril et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

López-Puc¹,

Góngora²,

Cano-Sosa³

et al. 2023

Trop Subtrop Agroecosyst

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Background: Chrysanthemum is one of the most popular landscape plants worldwide. Introducing desirable traits into this plant by conventional breeding has limitations due to cross-incompatibility. An approach to this problem is the introduction of resistance or tolerance traits via plant genetic transformation. In vitro regeneration of plants is necessary for implementing genetic transformation systems; therefore, regeneration via morphogenesis is required before any genetic transformation attempt. Objective: To induce morphogenesis from the leaf explant of Dendranthema grandiflora var Micromargara. Methodology: Micropropagation from node cuttings was induced from node cuttings of 3 cm in length from 6 months-old plants that were used as explants to produce seedlings as source explants. Morphogenesis experiments were done using leaf segments of 1 cm2 from 8-weeks-old in vitro seedlings. The explants were transferred to basal medium Murashige and Skoog at 4.4 gL-1 supplemented with different plant growth regulators. During the morphogenic process, leaves samples were collected to detect which morphogenic process was induced. Results: Node cuttings culture on Murashige and Skoog medium at 3.3 gL-1 supplemented with benzylaminopurine at 2.2 µM yielded a vegetative growth increase from 1 to 7 shoots, 2 to 12 leaves per node cutting, and the stem length of 3 to 9.5 cm. The leaf explant induced three ways of in vitro morphogenesis: direct and indirect roots organogenesis (rhizogenesis) and, shoot organogenesis. Direct rhizogenesis was induced in plant growth regulator-free Murashige and Skoog medium (18 roots/explant). Indirect rhizogenesis from leaf explant was less efficient than direct rhizogenesis, get obtaining six roots per leaf explant cultured on Murashige and Skoog medium at 4.4 g L-1 supplemented with 0.4 µM thidiazuron and 4.5 µM 2,4-dichlorophenoxyacetic acid, and three roots per leaf explant cultured on Murashige and Skoog medium supplemented with 0.4 µM thidiazuron and 9.05 µM 2,4-dichlorophenoxyacetic acid. Indirect shoot organogenesis was induced from leaf explants cultured on Murashige and Skoog at 4.4 g L-1 supplemented with 13.32 µM benzylaminopurine and 4.83 µM naphthalene acetic acid; 50% of explants with callus formed shoots (2 shoots/leaf explant). Through histological analysis it was possible to verify that the morphogenic response obtained was organogenesis. Implications: Regeneration of Dendranthema grandiflora var. Micromargara established herein will allow improvement of the variety through techniques of genetic transformation mediated by Agrobacterium and any other strategy to incorporate or silence genes of interest in the plant. Conclusion: The high capacity to induce roots of Dendranthema grandiflora var Micromargara, may provide an efficient model for further investigating the mechanism of rhizogenesis from leaf explants cultured without plant growth regulators.

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“…A large portion of soluble inorganic phosphate applied to the soil as chemical fertilizer is immobilized and becomes unavailable to crop plants. A large number of microorganisms present in the rhizosphere are known to solubilize insoluble phosphorus (Ranadev et al, 2019) [16] and make available to the plants and are called as phosphorus solubilizing microorganisms (PSM) which include phosphorus solubilizing bacteria (PSB) and phosphorus solubilizing fungi (PSF). Phosphorus solubilizing activity is carried out by a large number of saprophytic bacteria and fungi through various microbial processes or mechanisms including organic acid production and proton extrusion (Karpagam and Nagalakshmi, 2014) [11] .…”

Section: Introductionmentioning

confidence: 99%

Screening of efficient phosphate solubilising bacteria from different chickpea growing areas of Karnataka

Dakshayini¹,

Anantha²,

Gm³

et al. 2020

J Pharmacogn Phytochem

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Different Chickpea growing areas of Karnataka like Arasikere, Chamarajanagara, Mysore and Devanahalli were selected for the isolation and screening of phosphate solubilising bacteria. Totally 24 isolates were isolated from four soil samples of different Chickpea growing areas. Based on zone of solubilization ten isolates were selected to investigate their efficiency to solubilize insoluble phosphate in Pikovskaya's agar media under in vitro conditions. Among 10 isolates ACP-3 recorded higher PSE of 188.8% followed by ACP-2 (150.0%) and CCP-2 (145.4%). All the 10 isolates were tested for their ability to release percent Pi from tricalcium phosphate (TCP) in the Pikovskaya's broth media. Highest percent Pi released from TCP by the isolate ACP-3 (17.36%) followed by CCP-2 (14.14%). Same isolates were also tested for pH change in the Pikovskaya's broth at different days of intervals. ACP-3 recorded least pH which shows maximum decrease in pH of Pikovskaya's broth followed by ACP-2 and CCP-2. All the three experiments result shows that among the 10 isolates ACP-3 was found to be most efficient phosphate solubilising bacteria and this isolate was identified as Bacillus licheniformis from 16srRNA sequencing.

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Studies on Isolation, Characterization and in-vitro Screening of Plant Growth Promoting Rhizobacteria from Rhizospheric Soil of Chrysanthemum (Dendranthema grandiflora Tzvelev.)

Cited by 4 publications

References 7 publications

Synergistic Action of PGPR and Biofungicide with Active Ingredient of Cladosporium clasporioides to Control White Rust on Chrysanthemum

Synergistic Action of PGPR and Biofungicide with Active Ingredient of Cladosporium clasporioides to Control White Rust on Chrysanthemum

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

Screening of efficient phosphate solubilising bacteria from different chickpea growing areas of Karnataka

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