Studies on Linear Polymers from p-Substituted Phenol. II

Khobragade, Y. F.; Gupta, Mool C.

doi:10.1080/10601329508020315

Cited by 1 publication

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“…The design of multichannel biochips is underway, and this will facilitate the high-throughput, simultaneous synthesis of phenolic polymers and copolymers under a variety of reaction conditions and with a large number of phenolic substrates. One may envision that such highthroughput transformations on the microscale may be useful in rapidly identifying phenolic polymers for electronic materials (Khobragade and Gupta, 1995) or for sensor elements (Kim et al, 1998;Wu et al, 2000), as well as other biosynthetic products beyond those generated by peroxidase using a more complete biocatalytic repertoire.…”

Section: Polymer Growth On Microchannel Wallsmentioning

confidence: 99%

Microfluidic peroxidase biochip for polyphenol synthesis

Srinivasan

Lee

et al. 2002

Biotech & Bioengineering

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An enzyme-containing microfluidic biochip has been developed for the oxidative polymerization of phenols. The biochip consists of a simple T-junction with two feed reservoirs 20 mm apart and a microreaction channel 30 mm long. The channel is 15 microm deep and 200 microm wide at the center, giving a reaction volume of 90 nL. The biochip was fabricated using conventional photolithographic methods on a glass substrate etched using a HF-based solution. Fluid transport was enabled using electroosmotic flow. Soybean peroxidase was used as the phenol oxidizing catalyst, and in the presence of p-cresol and H(2)O(2), essentially complete conversion of the H(2)O(2) (the limiting substrate) occurred in the microchannel at a flow rate of ca. 290 nL/min. Thus, peroxidase was found to be intrinsically active even upon dramatic scale-down as achieved in microfluidic reactors. These results were extended to a series of phenols, thereby demonstrating that the microfluidic peroxidase reactor may have application in high-throughput screening of phenolic polymerization reactions for use in phenolic resin synthesis. Finally, rapid growth of poly(p-cresol) on the walls of the microreaction channel could be performed in the presence of higher H(2)O(2) concentrations. This finding suggests that solution-phase peroxidase catalysis can be used in the controlled deposition of polymers on the walls of microreactors.

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Section: Polymer Growth On Microchannel Wallsmentioning

confidence: 99%