Studies on lipid A isolated from Phyllobacterium trifolii PETP02T lipopolysaccharide

Zamlynska, Katarzyna; Komaniecka, Iwona; Żebracki, Kamil; Mazur, Andrzej; Sroka-Bartnicka, Anna; Choma, Adam

doi:10.1007/s10482-017-0872-0

Cited by 13 publications

(13 citation statements)

References 61 publications

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“…The transferase encoded by the gene designated as rgtF is responsible for the transfer of Gal p A from the donor dodecyl-P-GalA to lipid A, according to Brown and co-workers [ 11 ]. Similar genes have been identified in R. leguminosarum , M. loti , M. opportunistum , Caulobacter crescentus , Aquifex aeolicus , Azospirillum lipoferum [ 11 ], as well as in at least four strains from Phyllobacterium [ 13 ], and in many bacteria from the class α-proteobacteria, where, in several cases, structural studies have confirmed the presence of α-Gal p A at C-1 of lipid A ([ 14 ], and this work). Among them are also bradyrhizobia [ 15 ], photosynthetic Bradyrhizobium BTAi1 [ 16 ], and Rhodopseudomonas palustris BisA53 [ 17 ].…”

Section: Discussionmentioning

confidence: 60%

The Lipid A from the Lipopolysaccharide of the Phototrophic Bacterium Rhodomicrobium vannielii ATCC 17100 Revisited

Komaniecka

Suśniak

Choma

et al. 2020

IJMS

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The structure of lipid A from lipopolysaccharide (LPS) of Rhodomicrobium vannielii ATCC 17100 (Rv) a phototrophic, budding bacterium was re-investigated using high-resolution mass spectrometry, NMR, and chemical degradation protocols. It was found that the (GlcpN)-disaccharide lipid A backbone was substituted by a GalpA residue that was connected to C-1 of proximal GlcpN. Some of this GalpA residue was β-eliminated by alkaline de-acylation, which indicated the possibility of the presence of another so far unidentified substituent at C-4 in non-stoichiometric amounts. One Manp residue substituted C-4′ of distal GlcpN. The lipid A backbone was acylated by 16:0(3-OH) at C-2 of proximal GlcpN, and by 16:0(3-OH), i17:0(3-OH), or 18:0(3-OH) at C-2′ of distal GlcpN. Two acyloxy-acyl moieties that were mainly formed by 14:0(3-O-14:0) and 16:0(3-O-22:1) occupied the distal GlcpN of lipid A. Genes that were possibly involved in the modification of Rv lipid A were compared with bacterial genes of known function. The biological activity was tested at the model of human mononuclear cells (MNC), showing that Rv lipid A alone does not significantly stimulate MNC. At low concentrations of toxic Escherichia coli O111:B4 LPS, pre-incubation with Rv lipid A resulted in a substantial reduction of activity, but, when higher concentrations of E. coli LPS were used, the stimulatory effect was increased.

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Section: Discussionmentioning

confidence: 60%

The Lipid A from the Lipopolysaccharide of the Phototrophic Bacterium Rhodomicrobium vannielii ATCC 17100 Revisited

Komaniecka

Suśniak

Choma

et al. 2020

IJMS

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“…Stenotrophomonas , Bradyrhizobium , Sphingomonas , and Phyllobacterium bacteria were highly abundant in the MC group with low toxicity but can lead to human infection with severe immunodeficiency. They could all secrete lipopolysaccharides (LPS) − except Sphingomonas . LPS is one of the most potent inflammatory chemicals, acting on the Toll-like receptor (TLR-4) to induce the expression of inflammatory genes.…”

Section: Resultsmentioning

confidence: 99%

Collagen Peptides Isolated from Salmo salar and Tilapia nilotica Skin Accelerate Wound Healing by Altering Cutaneous Microbiome Colonization via Upregulated NOD2 and BD14

Mei

Liu

et al. 2020

J. Agric. Food Chem.

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Collagen peptides can promote wound healing and are closely related to microbiome colonization. We investigated the relationship among collagen peptides, wound healing, and wound microflora colonization by administering the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Tilapia nilotica skin collagen peptides (Tn-SCPs). We analyzed the vascular endothelial growth factor (VEGF), fibroblast growth factors (β-FGF), pattern recognition receptor (NOD2), antimicrobial peptides (β-defence14, BD14), proinflammatory (TNF-α, IL-6, and IL-8) and anti-inflammatory (IL-10) cytokines, macrophages, neutrophil infiltration levels, and microbial communities in the rat wound. The healing rates of the Ss-SCP- and Tn-SCP-treated groups were significantly accelerated, associated with decreased TNF-α, IL-6, and IL-8 and upregulated BD14, NOD2, IL-10, VEGF, and β-FGF. Accelerated healing in the collagen peptide group shows that the wound microflora such as Leuconostoc, Enterococcus, and Bacillus have a positive effect on wound healing (P < 0.01). Other microbiome species such as Stenotrophomonas, Bradyrhizobium, Sphingomonas, and Phyllobacterium had a negative influence and decreased colonization (P < 0.01). Altogether, these studies show that collagen peptide could upregulate wound NOD2 and BD14, which were implicated in microflora colonization regulation in the wound tissue and promoted wound healing by controlling the inflammatory reaction and increasing wound angiogenesis and collagen deposition.

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“…The substitution of only the reducing end of lipid A by α-(1→1)-d-GalpA is also not common among bacteria and has been identified in lipids A from a few representative genera. These include the associative diazotroph Azospirillum lipoferum [23], and some rhizobia: M. huakuii [10], M. loti [11], and P. trifolii [24]. On the other hand, the substitution of exclusively the non-reducing end of lipid A by α-d-GalpA-(1→ at position C-4' is not common either, and has been identified in lipids A from a few representatives of rhizobia (R. etli CE3, R. leguminosarum bvs.…”

Section: Discussionmentioning

confidence: 99%

Lipid A from Oligotropha carboxidovorans Lipopolysaccharide That Contains Two Galacturonic Acid Residues in the Backbone and Malic Acid A Tertiary Acyl Substituent

Choma

Zamlynska

Mazur

et al. 2020

IJMS

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The free-living Gram-negative bacterium Oligotropha carboxidovorans (formerly: Pseudomonas carboxydovorans), isolated from wastewater, is able to live in aerobic and, facultatively, in autotrophic conditions, utilizing carbon monoxide or hydrogen as a source of energy. The structure of O. carboxidovorans lipid A, a hydrophobic part of lipopolysaccharide, was studied using NMR spectroscopy and high-resolution mass spectrometry (MALDI-ToF MS) techniques. It was demonstrated that the lipid A backbone is composed of two d-GlcpN3N residues connected by a β-(1→6) glycosidic linkage, substituted by galacturonic acids (d-GalpA) at C-1 and C-4’ positions. Both diaminosugars are symmetrically substituted by 3-hydroxy fatty acids (12:0(3-OH) and 18:0(3-OH)). Ester-linked secondary acyl residues (i.e., 18:0, and 26:0(25-OH) and a small amount of 28:0(27-OH)) are located in the distal part of lipid A. These very long-chain hydroxylated fatty acids (VLCFAs) were found to be almost totally esterified at the (ω-1)-OH position with malic acid. Similarities between the lipid A of O. carboxidovorans and Mesorhizobium loti, Rhizobium leguminosarum, Caulobacter crescentus as well as Aquifex pyrophylus were observed and discussed from the perspective of the genomic context of these bacteria.

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Studies on lipid A isolated from Phyllobacterium trifolii PETP02T lipopolysaccharide

Cited by 13 publications

References 61 publications

The Lipid A from the Lipopolysaccharide of the Phototrophic Bacterium Rhodomicrobium vannielii ATCC 17100 Revisited

The Lipid A from the Lipopolysaccharide of the Phototrophic Bacterium Rhodomicrobium vannielii ATCC 17100 Revisited

Collagen Peptides Isolated from Salmo salar and Tilapia nilotica Skin Accelerate Wound Healing by Altering Cutaneous Microbiome Colonization via Upregulated NOD2 and BD14

Lipid A from Oligotropha carboxidovorans Lipopolysaccharide That Contains Two Galacturonic Acid Residues in the Backbone and Malic Acid A Tertiary Acyl Substituent

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