Studies on <i>In Vitro</i> Antioxidant Activity of Methanolic Extract and Fractions of <i>Ficus Hispida</i> Lin. Fruits

Abstract

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC 50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC 50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF. In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

Response Surface Optimization for Antioxidant Extraction from Jackfruit (<i>Artocarpus heterophyllus</i> Lam.) Seed and Pulp

“…The natural antioxidants found in fruits and vegetables have recently gained immense interest among food scientists, nutritionists and consumers because of their ability to reduce risk of chronic diseases [28]. Antioxidants extracted from the seeds and pulps of jackfruit have been reported to prevent or improve a lot of problems and diseases related to human health [1,2,29]. Numerous literatures are available on jackfruit-based food products and the health benefits of jackfruit [1,2,7,14,[30][31][32].…”

Unveiling the therapeutic potential: Evaluation of anti-inflammatory and antineoplastic activity of Magnolia champaca Linn’s stem bark isolate through molecular docking insights

“…Neutralization of reactive oxygen or hydrogen species by compounds with antioxidant properties is well known to protect cellular components and prevent chronic diseases such as neoplasms or cancer [ 9 ]. Researchers have found thousands of compounds to combat such damage and diseases, but the scarcity of safe, effective and target-specific therapies is the prime reason for the failure of cancer treatment, which contributes to such a large number of deaths [ [10] , [11] , [12] ]. Therefore, the search for any potential compound that will be safe and effective in cancer treatment is still a major focus of biological research.…”

“…Nature is always the predominant source of compounds used in therapies and has long been investigated by the scientific community [ 11 , 13 ]. Magnolia champaca Linn.…”

Lipid Lowering and Antioxidant Activities of Methanolic Extract of <i>Ficus hispida</i> Linn. Fruits in Cholesterol Fed Rats

“…But, according to best of our knowledge there is no scientific evidence on in vivo or in vitro antioxidant activity and lipid lowering activity of fruits of this plant. Very recently we have studied the in vitro antioxidant activity of the fruits of this plant and results showed that the methanol extract and fractions may have a significant antioxidant activity [18].…”