Studies on <i>In Vitro </i>Response to Callus Induction and Regeneration of Five High Yielding <i>Indica </i>rice Varieties

Abstract

Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media composition. The highest callus induction were observed in media containing Sucrose as a carbon source. Among those varieties Binadhan-6 and BRRI dhan-48 showed highest rate of callus induction respectively. This study will be useful for selecting suitable callus induction medium for callus induction in future that will be useful for not only national but also international plant breeders for producing new variety and so on.

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

“…The present findings are consistent with previous observations that different cultivars of rice exhibit a variable tendency for callus induction ( Zaidi et al, 2006 , Ge et al, 2006 , Nishimura et al, 2005 ). Genotypes, as widely accepted, are considered to be a major limitation which restricts the successful regeneration of rice cultivars ( Khan et al, 2019 ). A lot of literature has been published on optimized tissue culture protocols for rice, but they are mainly genotype-dependent.…”