Studies on Media for Enumerating Enterococci in Frozen Vegetables1

Splittstoesser, D. F.; Wright, R. C.; Hucker, G. J.

doi:10.1128/aem.9.4.303-308.1961

Cited by 7 publications

(5 citation statements)

References 6 publications

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“…for enterococcal isolation been compared simultaneously (e.g., Fanelli and Ayres, 1959;Splittstoesser, Wright, and Hucker, 1961). The only recent comparative study of media by use of the pour plate method is the work of Saraswat, Clark, and Reinbold (1963) on dairy products.…”

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confidence: 99%

Comparison of Direct Plating Media for the Isolation and Enumeration of Enterococci in Certain Frozen Foods1

Burkwall

Hartman

1964

Applied Microbiology

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BURKWALL, _MARY K. (Iowa State University, Ames), AND PAUL A. HARTMAN. Comparison of direct plating media for the isolation and enumeration of enterococci in certain frozen foods. Appl. MIicrobiol. 12:18-23. 1964.-A total of 15 agar media were examined for their yield, selectivity, readability, and simplicity of preparation and use. A thallium medium of Barnes was selected as the better of the high yield-fair selectivity type of medium and an azide-citrate medium of Reinbold appeared to be the better of the low Yield-high selectivity type of medium. Sodium carbonate (optimal concentration, 0.20%Z,) was found to increase recovery substantially when added to certain media, especially in the presence of 0.05% Tween 80. When these two ingredients were incorporated into a medium modified after Slanetz and Bartley, the resultant medium was superior to other media for the isolation and enumeration of enterococci in certain frozen foods, such as peas and hamburger, by the direct plating method.

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Comparison of Direct Plating Media for the Isolation and Enumeration of Enterococci in Certain Frozen Foods1

Burkwall

Hartman

1964

Applied Microbiology

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“…AD and EVA broths were not designed for use in marine waters (Litsky, personal communication); nonstreptococcal growth in both media has been observed (6,9,11,17). The present study also points to shortcomings in these media when used for other than the original purpose.…”

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confidence: 53%

Occurrence of False-Positive Most Probable Number Tests for Fecal Streptococci in Marine Waters1

Buck¹

1969

Applied Microbiology

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By the use of the most probable number technique with azide dextrose and ethyl violet azide broths for enterococci, the common occurrence of false-positive tests was noted when marine and estuarine waters were sampled. Organisms isolated included a marine bacterium, gram-positive and gram-negative nonmarine bacteria, and yeasts. All cultures were capable of growth in azide-dextrose, ethyl violet-azide, and KF broths. Representative isolates grew in media containing 0.08% NaN3. The tentatively accepted most probable number method for fecal streptococci is thus of dubious value in assessment of sewage pollution levels in estuarine waters. All positive tubes must be examined microscopically for the presence of nonstreptococcal forms.

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“…In a previous study of "total" count populations on frozen vegetables, 41 % of the organisms on snap beans and 75 % of those on whole kernel corn were gram-positive, catalase-negative cocci (19). Although many of the organisms resembled typical lactic streptococci, enterococci, and leuconostocs, more than 50% of the 860 isolates appeared to differ significantly from "type cultures" or from organisms described by other investigators.…”

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confidence: 76%

“…Azide-sensitive strains, able to grow in nonselective media, might well dilute out other types present in smaller numbers. Support for this conclusion comes from the fact that yellow streptococci have been recovered from New York vegetables when the cultures were isolated from azide dextrose and EVA broths (19).…”

Section: Discussionmentioning

confidence: 99%