Studies on Metal Microbe Interaction of Three Bacterial Isolates From East Calcutta Wetland

Adarsh, V.K.; Mishra, Madhusmita; Chowdhury, Sanhita; Sudarshan, M.; Thakur, Ashoke Ranjan; Chaudhuri, Shaon Ray

doi:10.3844/ojbsci.2007.80.88

Cited by 39 publications

(15 citation statements)

References 18 publications

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“…Adarsh et al (2007). Among the eleven metal salts checked for MIC determination, the intracellular accumulation using EDXRF analysis (Adarsh et al, 2007) (Jordan Valley EX 3600 EDXRF system) was carried out only for treatment with common environmental contaminants like Cu, Cr, Co, Ni and Pb.…”

Section: Antibiotic Assaymentioning

confidence: 99%

“…The initial identification of the pure isolates was on the basis of their morphology using light microscopy (1000X magnification on a Zeiss Axiostar Plus microscope) as well as Environmental scanning electron microscopy (FEI QUANTA 200 MARK 2 at 15 kV ) (Adarsh et al, 2007). The Gram nature of the isolate was determined by differential staining as per standard procedure.…”

Section: Morphological Characterizationmentioning

confidence: 99%

“…Since the objective was to isolate extracellular protease secreting bacteria, milk medium plates were used as selective media (Adarsh et al, 2007). 50 µL of water sample collected from the different sites were directly spread on the milk media plates and incubated at 37°C for overnight.…”

Section: Cultivation Medium and Growth Conditionsmentioning

confidence: 99%

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Bacterial Isolates of Marine Coast as Commercial Producer of Protease

Das¹

2012

OnLine Journal of Biological Sciences

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The objective of this work was to explore and exploit the extracellular protease secreting marine microbial biodiversity of the eastern coastal region of India. Culture dependent method was applied for isolation of microbes from the marine coast of West Bengal (Digha and Mandarmani) and Andhra Pradesh (Vizag) in India. Six protease secreting isolates were screened using casein hydrolysing property as well as azocasein assay and characterized on the basis of their morphological, biochemical, physiological and 16S rDNA based molecular properties. The enzymes were used for various commercial applications at a laboratory scale. Besides milk media and Luria Bertini broth, all the isolates grew in carbon minimal salt medium with jaggeri or tamarind as the carbon source (0.3% w/v). They showed intracellular metal accumulation when grown in presence of metal salts in the medium as evident from Energy Dispersive Xray Fluorescense Analysis (EDXRF) data. Maximum accumulation of lead was found in case of Bacillus cereus SM2. It showed equal efficiency of metal removal from solid strip at zero valent state. The isolates were also capable of complete removal of silver from exposed X-ray film after 48 hrs of incubation except for Escherichia coli SD1. Bacillus cereus SM2, isolate SD2 (closest to Bacillus pumilus) and isolate SV1 (closest to Bacillus cibi) were able to enhance the cleaning efficiency of detergent when used as additive. Use of tamarind and jaggeri as carbon source in minimal medium would make the process cost effective during large scale application. The ability to grow in a wide range of temperature and pH and accumulation of heavy metals revealed that these isolates would be potential candidates for bioremediation. Thus the marine diversity for protease production is extremely rich with immense commercial applications.

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Section: Antibiotic Assaymentioning

confidence: 99%

Section: Morphological Characterizationmentioning

confidence: 99%

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Bacterial Isolates of Marine Coast as Commercial Producer of Protease

Das¹

2012

OnLine Journal of Biological Sciences

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“…Morphological structures and Gram characteristics were confirmed using light microscopy (1000X magnification on a Zeiss Axiostar Plus microscope) as well as Environmental scanning electron microscopy (FEI QUANTA 200 MARK 2 at 15 kV ) (Adarsh et al, 2007). Gram character was reconfirmed by Real time PCR analysis (Shigemura et al, 2005).…”

Section: Characterization Of Isolatementioning

confidence: 99%

“…Water sample was collected from coastal region of Mandarmani (21° 37.012'N/87° 29.881'E), West Bengal, India and 50 µL of water was spread on milk media (Adarsh et al, 2007) plate to isolate protease secreting strain. The protease producing strain was subsequently purified by repeated streaking.…”

Section: Samplementioning

confidence: 99%

Degumming of Raw Silk Fabric With Help of Marine Extracellular Protease

Das

Sudarshan²,

Thakur

et al. 2013

American Journal of Biochemistry and Biotechnology

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Protease secreting microbe was isolated and characterized on the basis of their morphological, biochemical, physiological and 16S rDNA based molecular properties. The extracellular protease was quantified and characterized. Protease was used for different time (4, 8, 12 and 24 h) at different temperature (RT and 37°C) for optimization of the degumming process for raw silk fabric with enzyme dosage (0.2-1 unit/cm 2 of fabric). Post-enzymatic treatment, the fabric quality and texture was compared with conventionally treated as well as untreated fabric in terms of degumming loss, tensile strength and yarn count and colour fastness to light/water. The isolate SM1 (Bacillus thuringensis) was able to grow in Carbon Minimal Salt Medium (CMSM) with jaggery or tamarind as the carbon source (0.3% w/v). Energy Dispersive X-Ray Fluorescense (EDXRF) data showed intracellular accumulation of heavy metal by the isolate. Extracellular protease was able to degum silk fabric within 4 h at RT with enzyme concentration of 0.8unit/cm 2 and the maximum degumming loss was 21.72%. Post enzymatic degumming, a shiny texture was observed under Environmental Scanning Electron Microscope (ESEM) and the yarn volume also increased. Utilization of CMSM made the process cost effective during large scale application. Intracellular metal accumulation and growth in a wide range of temperature and pH made the isolate a potential candidate for bioremediation. Extracellular protease with significant degumming property could be used as an eco friendly approach as compared to the conventional chemical treatment.

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Arsenic Removal and Biotransformation Potential of Exiguobacterium Isolated From an Arsenic‐Rich Soil of Chhattisgarh, India

Pandey

Bhatt

2015

CLEAN Soil Air Water

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The presence of arsenic (As) in soil and groundwater has become a serious environmental issue of various regions of India. A haloalkaliphilic Exiguobacterium sp. (As-9) was isolated via enrichment culture technique from an arsenic-rich soil of the Rajnandgaon district in the state of Chhattisgarh, India, and characterized as a novel arsenic removing bacteria. The isolate proved to be capable of growing over a broad range of arsenic concentration and was resistant to relatively high levels of arsenate (700 mM) and arsenite (180 mM). It displayed great potential in removing about 99% arsenic under aerobic conditions from the aqueous environment in ten days at 37°C at 100 rpm. Electron microscopy revealed a fourfold increase in the cell size due to intracellular accumulation of arsenic. Selective enzyme assays confirmed that the two intracellular enzymes, namely arsenate reductase and arsenite oxidase played a significant role in arsenate reduction and arsenite oxidation, respectively, thereby contributing to the effective removal of arsenic. These results provide evidence for the occurrence of two distinct mechanisms involved in arsenic resistance; one via arsenate reduction and the other via arsenite oxidation. Moreover, the study reports for the first time the role of arsenic transforming bacteria from this region which could resist and transform exceptionally high concentrations of arsenic oxyanions. Such metabolically active bacterial strain may find application in the treatment of arsenic contaminated soil and aquifers in the near future.

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Studies on Metal Microbe Interaction of Three Bacterial Isolates From East Calcutta Wetland

Cited by 39 publications

References 18 publications

Bacterial Isolates of Marine Coast as Commercial Producer of Protease

Bacterial Isolates of Marine Coast as Commercial Producer of Protease

Degumming of Raw Silk Fabric With Help of Marine Extracellular Protease

Arsenic Removal and Biotransformation Potential of Exiguobacterium Isolated From an Arsenic‐Rich Soil of Chhattisgarh, India

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