Studies on mitochondria VI. The relationship between the structure, osmotic reactivity and ATPase activity of mitochondria from pigeon skeletal muscle

Harman, John W.; Kitiyakara, Amara

doi:10.1016/0014-4827(55)90119-1

Cited by 54 publications

(11 citation statements)

References 25 publications

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“…Fluoride added to the medium only partially inhibits the activity of phosphatases; that is, it reduces but does not eliminate the interference of dephosphorylating activities in the measurement of oxidative phosphorylation. On the other hand, a stimulation of the latent ATPase activity of mitochondria has been largely observed as a consequence of several treatments which damage the mitochondria and uncouple oxidative phosphorylation (22)(23)(24)(25)(26)(27)(28)(29). Some increase of the ATPase activity has also been observed, together with uncoupling of oxidative phosphorylation, in mitochondria from fatty liver, a condition in which mitochondria appear morphologically changed (25).…”

Section: O X I D a T I V E P H O S P H O R Y L A T I O N I N L I V E mentioning

confidence: 99%

Oxidative Phosphorylation in Mitochondria From Livers Showing Cloudy Swelling

Fonnesu

Severi

1956

The Journal of Cell Biology

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Changes in the morphology of mitochondria have been observed by several authors during the so-called "cloudy swelling" (1-6) which is a peculiar type of cell degeneration well known to pathologists. In view of the close correlation between the morphology and the enzymatic activity of mitochondria (7-9 and others), some metabolic disturbances occurring during cloudy swelling have been related to the changes in mitochondria (6,10, 11).Previous work has shown that phosphorylation of glucose is reduced in diphtheria toxin-treated animals, whose organs show a typical cloudy swelling (10). In these organs, easily hydrolyzable phosphate has been found to be decreased as compared with normal (11). Furthermore, it has been shown that the histological picture of cloudy swelling can be reproduced experimentally by injecting the animals with some compounds which uncouple oxidative phosphorylation, such as 2,4-dinitrophenol (12) and thyroxine (13).It appeared therefore reasonable to suppose that changes in oxidative phosphorylation could be implicated in the pathogenesis of the aforementioned cellular changes. The present paper concerns the oxidative phosphorylation in mitochondria isolated from livers showing cloudy swelling. Materials and MethodsAdult albino rats and guinea pigs, bred in the Institute of General Pathology, were used. In order to produce cloudy swelling of the liver, rats were injected intraperitoneally with the smallest dose of S. typhi murium toxin that was lethal within 4 days, and guinea pigs were injected subcutaneously with diphtheria toxin (1 ~.LD./250 gin. body weight). S. typld murlum toxin was prepared as described previously (6); a commercial preparation of diphtheria toxin was used. The animals were fasted for the 12 hours immediately before killing at 24 hours after the toxin injection.Preparation of Mitochondria.--All manipulations were performed in a cold room at 0°C. using chilled materials.The animals were stunned by a blow on the head, bled at the neck, and the liver was quickly excised. A small sample was taken for histological examination and the remainder

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Section: O X I D a T I V E P H O S P H O R Y L A T I O N I N L I V E mentioning

confidence: 99%

Oxidative Phosphorylation in Mitochondria From Livers Showing Cloudy Swelling

Fonnesu

Severi

1956

The Journal of Cell Biology

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“…A similar hypothesis was proposed to account for the swelling and shrinking of mitochondria in varied solute environments (8). It would explain the reported interactions between E. coli and concentrated citrate solutions, where an outflow of water from the cells coupled with the diffusional entry of citrate was observed but not explained (14).…”

Section: Discussionmentioning

confidence: 84%

Permeability of Serratia marcescens to Some Inorganic Salts

Zimmerman¹

1969

J Bacteriol

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The physical interactions between Serratia marcescens and solutions of NaCl, CaCl2, Cah2, Nal, and Na2HPO4 plus NaH2PO4 were examined. Dilute (0.017 N) salt solutions did not cause cells to lose water, as evidenced by the unchanged weight of centrifugally packed cells. The cells preferentially adsorbed the cations and repelled the anions of most salts in these solutions. Concentrated (1.71 N) salt solutions markedly reduced the weight and water content of centrifugally packed cells, although these cells took up considerable amounts of salts. More than 90% of the water in the packed-cell pellets was available for the solution of NaCl at 4.2 to 4.4% concentration. The observation that salts apparently penetrated the cells freely and yet caused extensive dehydration was not readily compatible with conventional concepts of solute-induced plasmolysis. Alternative hypotheses to explain the data included the following. First, the cells lost weight and water to concentrated salt solutions through a nonosmotic competitive dehydration, causing a shrinkage of the protoplasmic gel. The shrinkage ofthe cell wall was limited because of the rigidity of its mucopeptide layer; therefore, a space appeared between the cell wall and the cell membrane. Second, cells may have equilibrated their water activity with that of their environment by two mechanisms: (i) the loss of water by plasmolysis or competitive dehydration, and (ii) alterations in cell permeability that admitted previously excluded solutes to the cell interior. Possibly, the correct explanation of the observations reported here involves elements of all three hypotheses, plasmolysis, competitive dehydration, and permeability alterations.

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“…Aqueous HCHO was used. Its fixa~ tion of mitochondria results in the shape and size of the mitochondria which resemble the mitochondria in situ (Harman and Kitiyakara 1955;Witter, Watson, and Cottone 1955).…”

Section: (B) Potassium-sodium Replacementmentioning

confidence: 99%

Studies in the Metabolism of Plant Cells XI. the Donnan Equilibration and the Ionic Relations of Plant Mitochondria

Honda¹,

Robertson²

1956

Aust. Jnl. Of Bio. Sci.

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Ionic relations of mitochondria from red beetroot tissue (Beta vulgaris L.) sllsponded in various salt media were studied. Under the conditions of the experiments the mitochondrial suspensions behaved us a two-phase system, one coni,aining immobile ions and showing Donnan effects. Rome differences between mitochondria which showed simple Donnan charactoristics and those accumulating chloride ions aro described.

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Studies on mitochondria VI. The relationship between the structure, osmotic reactivity and ATPase activity of mitochondria from pigeon skeletal muscle

Cited by 54 publications

References 25 publications

Oxidative Phosphorylation in Mitochondria From Livers Showing Cloudy Swelling

Oxidative Phosphorylation in Mitochondria From Livers Showing Cloudy Swelling

Permeability of Serratia marcescens to Some Inorganic Salts

Studies in the Metabolism of Plant Cells XI. the Donnan Equilibration and the Ionic Relations of Plant Mitochondria

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