Studies on mitochondrial proteins I. Separation and characterization by polyacrylamide gel electrophoresis

Transport of proteins into mitochondria and chloroplasts.

“…So far, outer membranes have been isolated from rat liver (94,124), beef heart (62), Neurospora crassa (103), potato (91), and mung bean (91). The polypeptide patterns of these preparations are distinct from those of the inner membrane derived from the same source.…”

Proteins dealing with N‐ethylmaleimide inhibition of pig heart mitochondrial phosphate transport system

“…Polyacrylamide gel electrophoresis in SDS was carried out according to Weber and Osborn [17] and A 546 nm Packer [18] with some modifications: 6 × 120 nm gels with 6% acrylamide; 0.16% me thylenebisacrylamide; 0.1% ammonium persulfate; 0.05% TEMED; 0.1% SDS; 0.1% mercaptoethanol in 0.1 M sodium phosphate buffer pH 7.2.…”

Transfer of Monosaccharide onto Exogenous Acceptors by Mitochondrial Glycosyl Transferases

“…Calcium-binding mitochondrial glycoproteins have been described by Azzone et al [19], Gomez-Puyou et al [7] and Kimura et al [20]; McInick et ul. [21] have also in-vestigated mitochondrial glycoproteins. The Sottocasa glycoprotein was soluble and contained sialic acid, galactosamine, glucosamine, glucose and xylose ; the Lehninger glycoprotein was insoluble but also contained sialic acids, hexosamines and neutral sugars.…”