Studies on Mouse Monoclonal Anti-a and Anti-A, B Antibodies Obtained by Immunization With Blood Group a Secretor Saliva

Miyazaki, Seiko

doi:10.2974/kmj1951.38.473

Cited by 2 publications

(1 citation statement)

References 49 publications

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“…After 3 days, the spleen cells were fused with Sp2/O-Ag14 or P3 X63-Ag8.653 myeloma cells. Growth and subcloning of the hybridomas were carried out as described previously [23]. The immunoglobulin fraction purified from the culture supernatants of the hybridomas by DEAE Affi-Gel Blue column chromatography were used as monoclonal antibodies in this study.…”

Section: Production Of Mouse Monoclonal Antibodiesmentioning

confidence: 99%

Rapid Purification of Human DNase I Using Mouse Monoclonal Anti-DNase I Antibodies and Characterization of the Antibodies

Nakajima

Yasuda²,

Takeshita³

et al. 2000

Exp Clin Immunogenet

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Five anti-human deoxyribonuclease I (DNase I) monoclonal antibodies were obtained from BALB/c mice immunized with DNase I purified from human urine. Four of them inhibited DNase I enzyme activity, as did a rabbit polyclonal antibody; these 4 did not have immunostaining ability. The remaining one had immunostaining ability but no inhibitory activity. A Sepharose 4B column conjugated with 1 of the 4 antibodies that had inhibitory activity effectively adsorbed and eluted the DNase I enzyme; this did not occur with the rabbit polyclonal antibody. We showed that adding an immunoaffinity chromatography step made the purification of human DNase I easier and faster than the conventional procedure.

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Section: Production Of Mouse Monoclonal Antibodiesmentioning

confidence: 99%

Rapid Purification of Human DNase I Using Mouse Monoclonal Anti-DNase I Antibodies and Characterization of the Antibodies

Nakajima

Yasuda²,

Takeshita³

et al. 2000

Exp Clin Immunogenet

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Difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the ABH antigens in human tissues

et al. 1990

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Twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-A, -B and -H antibodies for detecting the corresponding antigens in selected human tissues. Although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. Helix asparsa agglutinin (HAA), Helix pomatia agglutinin (HPA) and monoclonal anti-A antibody recognized A antigens in the mucous cells of salivary glands from blood group A or AB nonsecretor as well as secretor individuals, whereas Dolichos biflorus agglutinin (DBA), Griffonia simplicifolia agglutinin-I (GSA-I), Sophora japonica agglutinin (SJA) and Vicia villosa agglutinin (VVA) did not bind to them from nonsecretors. A antigens in endothelial cells, lateral membrane of pancreatic acinar cells and small mucouslike cells of submandibular glands from some individuals were likewise recognized by HAA and HPA but not by other blood group A-specific lections. In contrast, both HAA and HPA did not recognize the A antigens in mucous cells of Brunner's glands while other A-specific lectins and monoclonal anti-A antibody reacted specifically with the antigens. Such a difference was not observed with lectins specific for blood group B. However, the B antigens in Brunner's glands were recognized by these lectins but not with monoclonal anti-B antibody. The difference in labelling ability was also noted among the blood group H-specific lectins and monoclonal anti-H antibody in endothelial cells of blood vessels. Ulex europaeus agglutinin-I reacted with these cells irrespective of ABO and the secretor status of the individuals, while Anguilla anguilla agglutinin and monoclonal anti-H antibody reacted only with those cells from blood group O individuals. No reaction was observed with Lotus tetragonolobus agglutinin in these tissue sites. These results suggest a great diversity of blood group antigens in different human tissues.

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Studies on Mouse Monoclonal Anti-a and Anti-A, B Antibodies Obtained by Immunization With Blood Group a Secretor Saliva

Cited by 2 publications

References 49 publications

Rapid Purification of Human DNase I Using Mouse Monoclonal Anti-DNase I Antibodies and Characterization of the Antibodies

Rapid Purification of Human DNase I Using Mouse Monoclonal Anti-DNase I Antibodies and Characterization of the Antibodies

Difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the ABH antigens in human tissues

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