Studies on nerve cell affinity of chitosan-derived materials

Abstract

Reparation of the central nervous system (CNS) is important because when it is impaired its recovery is difficult and concomitant malfunction of other parts of body occurs. In our previous studies, chitosan was found to be a good material supporting nerve repair. The purpose of this article was to study the ability of chitosan and some chitosan-derived materials to facilitate the growth of nerve cells. Those materials were chitosan, glutaraldehyde-crosslinked chitosan, glutaraldehyde-crosslinked chitosan-gelatin conjugate, a chitosan-gelatin mixture, chitosan coated with polylysine (CAP), and a chitosan-polylysine mixture (CPL). Gelatin and polylysine were used as controls. After nerve cells (gliosarcoma cells and normal cerebral cells) were grown on those materials, their attachment, spread, and growth were observed. The adsorption of some extracellular matrix molecules such as laminin and fibronectin on the materials and the role the molecules play in nerve cell attachment and spreading were also studied by enzyme-linked immunosorbent assay and MTT method. We found that both CAP and CPL have excellent nerve cell affinity, defined as the ability to promote nerve cell to grow and function normally. Those two materials may be promising for the repair of the nervous system. Materials precoated with laminin, fibronectin, and serum were analyzed for their nerve cell affinity. Results suggest that after being precoated with laminin and fibronectin solution or serum, all material have better nerve cell affinity.

Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer

“…It was already reported that besides its good biocompatibility, chitosan has an excellent nerve cell affinity. Extracellular molecules such as laminin, fibronectin, and other serum proteins have a good affinity to chitosan [20]. Although there were some differences in the cellular adhesion and proliferation rate ARTICLE IN PRESS Fig.…”

Differentiation of Human Adipose-Derived Stem Cells into Neuron-Like Cells Which Are Compatible with Photocurable Three-Dimensional Scaffolds

“…22 Haipeng et al reported that neurons cultured on the chitosan membrane grew well and that chitosan tube promote repair of the peripheral nervous system. 23 Yuan et al found that chitosan fibers supported the adhesion, migration, and proliferation of Schwann cells, which provide a similar guide for regenerating axons to Büngner bands in the nervous system. 24 However, to the best of our knowledge, no report has been appeared yet on chitosan-based scaffolds for the differentiation of hADSC into neuron-like cells (hADSC-NLCs).…”

Stroke Repair via Biomimicry of the Subventricular Zone

“…Chitosan is a biodegradable, inexpensive material with low toxicity that can be modified through incorporation of ECM proteins to the surface, including laminin and fibronectin that regulate cell functions (Haipeng et al, 2000;Cheng et al, 2007;Yang et al, 2010). Accordingly, due to its ability to be modified, chitosan carriers have demonstrated NSC survival, proliferation, and cell differentiation into desired phenotypes (Yang et al, 2010).…”