Studies on neurosteroids XVII.

Higashi, Tatsuya; Takido, Natsuko; Shimada, Kaoru

doi:10.1016/j.steroids.2004.08.001

Cited by 69 publications

(37 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3α,5α-THP is also formed centrally when ovarian and/or adrenal P 4 is metabolized in brain [21, 22] and it is also a neurosteroid [23, 24]. Neurosteroids are produced de novo in brain independent of peripheral gland secretion, they have paracrine effects, which typically occur at non-traditional steroid substrates, and rapid changes in levels of neurosteroids have been demonstrated in response to environmental and/or behavioral stimuli [25,26,27,28,29,30,31,32]. Neurosteroids are synthesized from cholesterol that binds mitochondrial benzodiazepine receptors, which facilitates transport to the inner mitochondrial membrane and initiates neurosteroid biosynthesis [33].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to some steroids that exhibit traditional actions via intracellular steroid receptors, most neurosteroids have actions via modulatory effects on neurotransmitter receptor activity, most notably at GABA A receptors [5, 34]. Exposure to extreme stimuli such as cold-water swim, restraint, footshock, carbon dioxide inhalation, ether exposure, or loud noise enhances neurosteroidogenesis [25,26,27,28,29,30,31,32]. As well, mating stimuli can also increase 3α,5α-THP levels to those which can produce agonist-like actions at GABA A receptors [35].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Progestin Concentrations Are Increased following Paced Mating in Midbrain, Hippocampus, Diencephalon, and Cortex of Rats in Behavioral Estrus, but Only in Midbrain of Diestrous Rats

Frye

Rhodes²

2006

Neuroendocrinology

View full text Add to dashboard Cite

Background: The progesterone (P₄) metabolite, 5α-pregnan-3α-ol-20-one (3α,5α-THP), acts in the midbrain ventral tegmental area (VTA) to modulate the intensity and duration of lordosis. 3α,5α-THP can also have anti-anxiety and anti-stress effects in part through actions in the hippocampus. Separate reports indicate that manipulating 3α,5α-THP levels in the VTA or hippocampus respectively can influence lordosis and affective behavior. 3α,5α-THP levels can also be altered by behavioral experiences, such as mating or swim stress. Whether endogenous levels of 3α,5α-THP modulate and/or are increased in response to affective and/or reproductively-relevant behaviors was investigated. Methods: In Experiment 1, rats in behavioral estrus or diestrus were individually tested sequentially in the open field, elevated plus maze, partner preference, social interaction, and paced mating tasks and levels of 17β-estradiol (E₂), P₄, dihydroprogesterone (DHP), and 3α,5α-THP in serum, midbrain, hippocampus, diencephalon, and cortex were examined. In Experiments 2 and 3, rats in behavioral estrus or diestrus, were individually tested in the battery indicated above, with, or without, paced mating and tissues were collected immediately after testing for later assessment of endocrine measures. Results: In Experiment 1, behavioral estrous, compared to diestrous, rats demonstrated more exploratory, anti-anxiety, social, and reproductive behaviors, and had higher levels of E₂ and progestins in serum, midbrain, hippocampus, diencephalon, and cortex. In Experiment 2, in midbrain and hippocampus, levels of 3α,5α-THP and its precursor DHP were increased among rats in behavioral estrus that were mated. In diencephalon, and cortex, DHP levels were increased by mating. In Experiment 3, in midbrain, levels of 3α,5α-THP and its precursor DHP were increased among diestrous rats that were tested in the behavioral battery with mating as compared to those tested in the behavioral battery without mating. Conclusions: Increased levels of 3α,5α-THP in behavioral estrus versus diestrous rats are associated with enhanced exploratory, anti-anxiety, social, and reproductive behaviors. Rats in behavioral estrus that are mated have further increases in 3α,5α-THP and/or DHP levels in midbrain, hippocampus, diencephalon, and cortex than do non-mated rats in behavioral estrus, whereas diestrous rats only show 3α,5α-THP increases in midbrain in response to behavioral testing that included mating.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Progestin Concentrations Are Increased following Paced Mating in Midbrain, Hippocampus, Diencephalon, and Cortex of Rats in Behavioral Estrus, but Only in Midbrain of Diestrous Rats

Frye

Rhodes²

2006

Neuroendocrinology

View full text Add to dashboard Cite

show abstract

“…Many derivatization reagents, such as N,N-dimethylaminonaphtalenesulfonyl (Dansyl) chloride, 25,26 p-nitrobenzoyl chloride, 27,28 pentafluorobenzyl bromide, 29,30 and 2-fluoro-Nmethylpyridinium-p-toluenesulfonate 31,32 for hydroxyl groups of steroid and lipid, and Girard-P, 33,34 O-ethylhydroxylamine hydrochloride, 35 2-hydrizino-N-methylpyridine, 36,37 and 2-nitro-4-trifluoromethylhydrazine 38,39 for steroidal carbonyl groups are reported. Ding et al, 40 Ali et al, 41 and Elba et al 42 recently reported that derivatization with silylating agents, such as BSTFA with 1% trimethylchlorosilane (TMCS), significantly increased sensitivity and selectivity by GC-MS detection of phenolic compounds, including estrogenic chemicals.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Detection and Structural Characterization of Flavonoids by Complexation with N,O-Bis(trimethysilyl)trifluoroacetamide Using Electrospray Ionization Mass Spectrometry

2008

View full text Add to dashboard Cite

“…AP and related neurosteroids in the brain have been recently measured by liquid chromatography (LC)-mass spectrometry (MS) coupled with electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) due to its selectivity, versatility and simultaneous multi-analyte quantification capability. 3,6,7 We have reported a validated LC-electron capture APCI (ECAPCI)-MS assay for the determination of the brain 3α,5α-Adiol after converting it to an electron-affinitive derivative. 8 Although ECAPCI-MS was a highly sensitive technique, this ionization occurred only when the ThermoQuest LCQ mass spectrometer was used, and it was unusable for other mass spectrometers, such as the Applied Biosystems API series instruments; the method has limitations on its versatility.…”

Section: Introductionmentioning

confidence: 99%

Studies on Neurosteroids XXI: An Improved Liquid Chromatography–Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains

et al. 2007

Self Cite

View full text Add to dashboard Cite

A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method for the determination of the rat brain 5α-androstane-3α,17β-diol (3α,5α-Adiol) has been developed and validated. The brain extract was purified using solid-phase extraction cartridges, derivatized with isonicotinoyl azide, and subjected to LC-MS/MS. The method was accurate and reproducible, and the limit of quantitation was 0.1 ng/g tissue when a 100-mg tissue sample was used. The change in the brain 3α,5α-Adiol level by immobilization stress was also analyzed using the developed method.

show abstract

Studies on neurosteroids XVII.

Cited by 69 publications

References 36 publications

Progestin Concentrations Are Increased following Paced Mating in Midbrain, Hippocampus, Diencephalon, and Cortex of Rats in Behavioral Estrus, but Only in Midbrain of Diestrous Rats

Progestin Concentrations Are Increased following Paced Mating in Midbrain, Hippocampus, Diencephalon, and Cortex of Rats in Behavioral Estrus, but Only in Midbrain of Diestrous Rats

Enhanced Detection and Structural Characterization of Flavonoids by Complexation with N,O-Bis(trimethysilyl)trifluoroacetamide Using Electrospray Ionization Mass Spectrometry

Studies on Neurosteroids XXI: An Improved Liquid Chromatography–Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains

Contact Info

Product

Resources

About