Studies on occurrence, molecular detection and genotyping of<i>Cryptosporidium parvum</i>along with associated risk factors in cattle and human from Aizawl district, Mizoram, India

Das, Pradeep; Deka, Devajani; Borthakur, Sonjoy Kumar; Roychoudhury, P.; Das, M.

doi:10.1080/09291016.2018.1526501

Cited by 6 publications

(8 citation statements)

References 19 publications

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“…Our data showed that Cryptosporidium infection was significantly higher among cattle aged 12 months and above compared to animals that were 12 months old and younger. This report is contrary to many other reports which suggested that Cryptosporidium infections were higher in younger animals than adults [ 35 , 37 , 38 ]. This is quite unexpected since younger animals, especially calves, are known to have reduced immunity and are therefore susceptibly prone to infection which normally leads to diarrhoea and other manifestations.…”

Section: Discussioncontrasting

confidence: 99%

“…A report from the Oyo state in Nigeria using ELISA and molecular methods found no significant difference in the prevalence of the parasite between nondiarrhoeic and diarrhoeic stool samples of cattle [ 37 ]. Also, the current finding is contrary to other studies in India and Ethiopia which reported a significantly higher prevalence among animals with diarrhoeic samples compared to those with nondiarrhoeic samples [ 35 , 36 ]. The observation of a high number of diarrhoeic samples from adult animals compared to young ones may be because young animals formed a smaller proportion of sample in the study.…”

Section: Discussioncontrasting

confidence: 99%

“…The prevalence rate for the current study was however higher than a similar study in Addis Ababa, Ethiopia, which reported a rate of 18.6% using both modified Ziehl-Neelsen staining and molecular techniques [ 22 ]. A study in Aizawl district in India using both modified Ziehl-Neelsen and molecular detection [ 35 ] and one other in Malaysia peninsular using only molecular detection method for Cryptosporidium [ 15 ] also recorded rates of 13% and 12.5%, respectively, which were lower compared to the present study. The varying prevalence of Cryptosporidium infection observed among cattle in different studies may be attributed to several reasons including season of sampling, the geographic and ecological location of the study areas, laboratory techniques employed in the detection of the parasite, type of animal management system practiced by the farmers, the breed of the animals, and hygiene status of the farms or kraals as well as the climate of the area under study [ 15 , 16 , 29 , 36 ].…”

Section: Discussioncontrasting

confidence: 91%

“…Prevalence of Cryptosporidium species was also higher in female cattle than in their male counterparts, although the difference was not statistically significant ( p = 0.716). This report is comparable to a study done in the coastal and forest savannah transition zone of the Greater Accra region of Ghana [ 36 ] and in the Aizawl district in India [ 35 ] and another one among native cattle herds in Nigeria [ 37 ]. It is however contrary to a study in Nigeria which recorded a significantly higher prevalence of Cryptosporidium among male animals [ 37 , 40 ].…”

Section: Discussionsupporting

confidence: 86%

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Cryptosporidium Infection and Associated Risk Factors among Cattle in the Central Region of Ghana

Dankwa

Feglo

Nuvor

et al. 2021

Journal of Parasitology Research

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Cryptosporidium species infects a wide number of animals including livestock all over the world. The current study was done to determine the prevalence and risk factors of Cryptosporidium infection among cattle in the Central Region of Ghana. Two hundred and eighty-seven (287) faecal samples were randomly collected from animals on eight cattle farms in four districts across two agroecological zones. A commercial enzyme-linked immunosorbent assay kit (CoproELISA, Savyon® Diagnostics Ltd., Israel) for Cryptosporidium was used in the detection of Cryptosporidium antigens in faecal samples. Characteristics of the animals such as age, sex, and location, as well as consistency of faecal samples, were collected. Pearson’s chi-square or Fisher’s exact test was used to determine the association between explanatory variables and Cryptosporidium infection while a logistic regression model was also used to determine the risk of infection. The overall prevalence of Cryptosporidium infection was 23.7% (95% CI, 18.7-28.6). Prevalence was significantly higher ( p = 0.049 ) among cattle aged 12-month old and above compared to those under 12 months of age. Among the four districts in the study area, Cape Coast metropolis recorded a significantly higher prevalence (60.5%; CI, 49.3-71.8), ( p < 0.001 ) compared to the other three. Furthermore, a significant association was observed between the consistency of faecal samples and Cryptosporidium infection ( p = 0.042 ). The prevalence of Cryptosporidium infection was also significantly higher among cattle from the coastal savanna zone (26.9%; 95% CI, 21.0-32.8) compared to those from the semideciduous forest area ( p = 0.017 ). Cattle in the forest zone had a lower risk of being infected with the parasite compared to those from the coastal savanna zone (OR 0.408; 95% CI, 0.182-0.915). In conclusion, Cryptosporidium was prevalent among cattle in the Central Region of Ghana. A higher prevalence of Cryptosporidium infection occurred in older animals and among animals in the coastal agroecological zone. The area of location and age of animals were identified as risk factors for Cryptosporidium infection in the Central Region of Ghana.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 91%

Section: Discussionsupporting

confidence: 86%

See 2 more Smart Citations

Cryptosporidium Infection and Associated Risk Factors among Cattle in the Central Region of Ghana

Dankwa

Feglo

Nuvor

et al. 2021

Journal of Parasitology Research

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“…Concerning sex, the present study showed no significant difference between male and female infection rates (p > 0.05). This result agreed with Das et al (2018), who found no significant variation (p > 0.05) in the sex-wise occurrence of Cryptosporidium in cattle. Similarly, no sex preponderance of Cryptosporidium was noted in sheep and goats in Kuwait (Majeed et al, 2018).…”

Section: Molecular Characterization Of Cryptosporidium Isolatessupporting

confidence: 92%

Prevalence and molecular characterization of Cryptosporidium sp. in ruminant livestock in South Sinai, Egypt

Taha

Barghash²,

SERAG

2022

Bulletin of Faculty of Science, Zagazig University

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Cryptosporidium is apicomplexan parasite that can infect a wide range of animal species as well as humans causing life-threatening diarrhoea, especially in young hosts. In Egypt, few studies on the molecular characterization of Cryptosporidium have been described in humans, but few data were available in animals has been performed until now. The present study aimed to detect and characterize Cryptosporidium spp. for the first time in 195 faecal specimens from ruminant animals of different sites from the South Sinai Governorate. The study was carried out from October 2020 to October 2021 using Ziehl-Neelsen fast stain (ZN) and a nested-PCR (nPCR) that targeted the Cryptosporidium oocyst wall protein (COWP) gene. Results showed an overall prevalence rate of infection in 105/195 (53.85%) and 86 /195 (43.59%) using ZN and nPCR, respectively. It was (68.6%, 50%, and 48.2%) by ZN and (49%, 53%, and 39.2%) by nPCR in cattle, goats, and sheep, respectively. Host-species, age, and status of faecal samples were risk factors for cryptosporidiosis, whereas location, sex, and clinical state have no significant difference (p> 0.05). The infections were due to C. parvum, which is dominant in Egyptian isolates preserved in GenBank data, and no correlation between the genotype and the geographic origins. We concluded that the presence of potentially zoonotic species of Cryptosporidium in ruminants in this region suggest that livestock could potentially contribute to human cryptosporidiosis in the study area. Further molecular studies on local human populations are required to understand the transmission dynamics of Cryptosporidium spp. in that region.

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Occurrence and factors associated with Cryptosporidium infection in livestock in three districts of Zambia

Mwaba,

Robertson,

Tembo

et al. 2024

Veterinary Parasitology: Regional Studies and Reports

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Studies on occurrence, molecular detection and genotyping ofCryptosporidium parvumalong with associated risk factors in cattle and human from Aizawl district, Mizoram, India

Cited by 6 publications

References 19 publications

Cryptosporidium Infection and Associated Risk Factors among Cattle in the Central Region of Ghana

Cryptosporidium Infection and Associated Risk Factors among Cattle in the Central Region of Ghana

Prevalence and molecular characterization of Cryptosporidium sp. in ruminant livestock in South Sinai, Egypt

Occurrence and factors associated with Cryptosporidium infection in livestock in three districts of Zambia

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