This work discusses issues concerning stability, tuning and dynamics of convergence of observer-based kinetics estimators. The analysis focuses on both continuous and discrete time formulations of the estimation algorithms. Concerning the former, it is shown that, with proper tuning, stability can be guaranteed, while simultaneously imposing a desired quasi-time invariant second order time response for the convergence of estimates to true values. Concerning the latter, an algorithm is presented, based on a forward Euler discretisation, whose error system is shown to be linear time-invariant. Furthermore, stability conditions were derived, which define the stable domain for the discretisation period as function of the tuning parameters. The theory is illustrated with a case-study of Baker's yeast fermentation. Results clearly confirm the theoretical developments. In particular, results concerning the stability domain for the Euler-based discrete formulation of the estimator are shown to have relevant practical implications.