Studies on pathogenic <i>Vibrio parahaemolyticus</i> during a warm weather season in the Seto Inland Sea, Japan

Alam, M.J.; Miyoshi, Shin Ichi; Shinoda, Sumió

doi:10.1046/j.1462-2920.2003.00458.x

Cited by 41 publications

(22 citation statements)

References 27 publications

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“…When detected, concentrations of the tdh ϩ strain have been reported to be Ͻ13/g (10,16,30). Low densities of tdh ϩ trh ϩ strains have also been reported from Japan and Chile (2,20). However, significantly higher densities have been reported in oysters from one study location in India where tdh was detected in 10.2% of strains and trh in 60% of strains isolated from oysters (14).…”

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confidence: 92%

Genetic Diversity of Clinical and Environmental Vibrio parahaemolyticus Strains from the Pacific Northwest

Paranjpye

Hamel

Stojanovski

et al. 2012

Appl Environ Microbiol

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Since 1997, cases of Vibrio parahaemolyticus-related gastroenteritis from the consumption of raw oysters harvested in Washington State have been higher than historical levels. These cases have shown little or no correlation with concentrations of potentially pathogenic V. parahaemolyticus (positive for the thermostable direct hemolysin gene, tdh) in oysters, although significant concentrations of tdh ؉ V. parahaemolyticus strains were isolated from shellfish-growing areas in the Pacific Northwest (PNW). We compared clinical and environmental strains isolated from the PNW to those from other geographic regions within the United States and Asia for the presence of virulence-associated genes, including the thermostable direct hemolysin (tdh), the thermostable-related hemolysin (trh), urease (ureR), the pandemic group specific markers orf8 and toxRS, and genes encoding both type 3 secretion systems (T3SS1 and T3SS2). The majority of clinical strains from the PNW were positive for tdh, trh, and ureR genes, while a significant proportion of environmental isolates were tdh ؉ but trh negative. Hierarchical clustering grouped the majority of these clinical isolates into a cluster distinct from that including the pandemic strain RIMD2210633, clinical isolates from other geographical regions, and tdh ؉ , trh-negative environmental isolates from the PNW. We detected T3SS2-related genes (T3SS2␤) in environmental strains that were tdh and trh negative. The presence of significant concentrations of tdh ؉ , trhnegative environmental strains in the PNW that have not been responsible for illness and T3SS2␤ in tdh-and trh-negative strains emphasizes the diversity in this species and the need to identify additional virulence markers for this bacterium to improve risk assessment tools for the detection of this pathogen.

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mentioning

confidence: 92%

Genetic Diversity of Clinical and Environmental Vibrio parahaemolyticus Strains from the Pacific Northwest

Paranjpye

Hamel

Stojanovski

et al. 2012

Appl Environ Microbiol

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“…Sample 1, water used as a negative control; and samples 2-8 represent periodic enrichment cultures, i.e., 8-, 7-, 6-, 5-, 4-, 3-and 2-h enrichment culture of V. parahaemolyticus ATCC 17802, respectively. Utilization of the enrichment-PCR method 27,28) also requires more time than the newly developed assay of this study, as additional gel-run and detection procedures are required after PCR step which is avoided by the use of real-time turbidimeter in the LAMP assay. A recent study has reported successful utilization of real-time PCR method after a 6 h enrichment period in detecting less than 5 V. parahaemolyticus cells/g of seafood, 29) however, this method is more expensive and complex, and requires more time than the LAMP assay of the present study.…”

Section: Specificity and Sensitivity Of Lamp Detectionmentioning

confidence: 99%

Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay Combined with Enrichment Culture for Rapid Detection of Very Low Numbers of Vibrio parahaemolyticus in Seafood Samples

Neogi

et al. 2015

Biol. Pharm. Bull.

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The aim of this study was to develop and evaluate a rapid and effective method to detect Vibrio parahaemolyticus, a leading pathogen causing seafood-borne gastroenteritis. A newly designed loop-mediated isothermal amplification (LAMP) assay including a short enrichment period was optimized. This assay correctly detected all the target strains (n 61) but none of the non-target strains (n 34). Very low numbers of V. parahaemolyticus (2 colony forming unit (CFU) per gram of seafood) could be detected within 3 h and the minimum time of the whole assay was only 5 h. Comparative screening of various seafood samples (n 70) indicated that the LAMP assay is superior to polymerase chain reaction (PCR) and conventional culture methods because it is more rapid and less complex. This highly sensitive LAMP assay can be applicable as the method of choice in large-scale and rapid screening of seafood and environmental samples to detect V. parahaemolyticus strains.Key words Vibrio parahaemolyticus; loop-mediated isothermal amplification; rapid detection; enrichment culture; seafood Vibrio parahaemolyticus is one of the most important seafood-borne pathogen causing gastrointestinal disorders to humans. This halophilic Gram-negative bacterium was first identified as a causative agent of human gastroenteritis in Japan in 1950.1) However, V. parahaemolyticus can occur ubiquitously in the brackish coastal environment of most continents and infections associated with seafood contaminated by this pathogen have occurred throughout the world.2-4) Particularly, the worldwide spread of the V. parahaemolyticus O3 : K6 serotype after its emergence in Asia in 1995, it has become very important to identify this pathogenic species in a rapid and sensitive manner.5) At present, the outbreak of V. parahaemolyticus infections is a significant public health concern in many countries including China, Japan and U.S.A. 6,7)Seafood is very popular in many countries, and simple and specific identification of a pathogen from seafood samples is essential for taking preventive and curative measures. Conventional methods for the diagnosis of V. parahaemolyticus include cultivation of bacteria on selective media followed by biochemical tests. However, the traditional phenotypic identification is problematic, it is very time-consuming requiring 3-7 d and not highly specific because several Vibrio species display similar biochemical characteristics, which make it difficult for rapid identification of V. parahaemolyticus. 8) To circumvent this problem, the identification of V. parahaemolyticus by rapid and specific molecular techniques targeting the genes encoding thermostable direct haemolysin (TDH) and TDH-related haemolysin (TRH) were developed.9-11) However, these genes are only found in pathogenic strains and most V. parahaemolyticus isolates from the environment sources do not produce TDH or TRH. 3,12) Besides, there are reports of toxigenic factors other than TDH or TRH, e.g., type III secretion system 2, associated with clinical V. parahaemolyticus stra...

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“…These workers had earlier detected toxigenic Vibrio cholerae from environmental water samples by an enrichment broth, semi-nested PCR procedure (Theron et al, 2000). Sea water and organic material analysed (Alam et al, 2003) to determine Vibrio parahaemolyticus, a potentially pathogenic bacterium, showed over 22% of samples positive for V. parahaemolyticus than the conventional (Most Probable Number) MPN culture technique could detect. PCR has already proven valuable in the screening of rhizobacteria for 1-amino-cyclopropane-1-carboxylic (ACC) deaminase (Babalola et al, 2003).…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Molecular techniques: An overview of methods for the detection of bacteria

Babalola¹

2003

Afr. J. Biotechnol.

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Several DNA molecular markers are now available for use in surveillance and investigation of foodborne outbreaks that were previously difficult to detect. The results from several sources of literature indicate substantially different degrees of sensitivities between conventional detection methods and molecular-based methods. The new technology is noted for increased sensitivity over the traditional culture methods which they complement.

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Studies on pathogenic Vibrio parahaemolyticus during a warm weather season in the Seto Inland Sea, Japan

Cited by 41 publications

References 27 publications

Genetic Diversity of Clinical and Environmental Vibrio parahaemolyticus Strains from the Pacific Northwest

Genetic Diversity of Clinical and Environmental Vibrio parahaemolyticus Strains from the Pacific Northwest

Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay Combined with Enrichment Culture for Rapid Detection of Very Low Numbers of Vibrio parahaemolyticus in Seafood Samples

Molecular techniques: An overview of methods for the detection of bacteria

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