Studies on Pesticides Mixture Degradation by White Rot Fungi

Gouma, Sofia; Papadaki, Anastasia; Markakis, George; Magan, Naresh; Goumas, Dimitrios E.

doi:10.12911/22998993/94918

Cited by 17 publications

(2 citation statements)

References 33 publications

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“…[56], Pseudomonas syringae [57] and B. subtilis [12]. Fungal laccase enzyme for degradation of liluron, chlorpyrifos and metribuzin was reported by Gouma, [58]. The release of extracellular laccase from Bacillus cereus allows degradation and decolorization of dye [59].…”

Section: Discussionmentioning

confidence: 99%

Differential analysis of pesticides biodegradation in soil using conventional and high-throughput technology

Gangola

Joshi²,

Kumar

et al. 2021

Preprint

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A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 ppm) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in minimal salt medium with 94, 91, 89 and 86% respectively as revealed by HPLC and GC analysis after 15 days of incubation. Presence of cyclobutane, pyrrolidine, chloroacetic acid, formic acid and decyl ester as major intermediate metabolites of cypermethrin biodegradation was observed in GC-MS analysis. Results based on 16S rDNA sequencing, and phylogenetic analysis showed maximum similarity of 2D with Bacillus cereus (MH341691). Stress responsive and catabolic/ pesticide degrading proteins were over expressed in the presence of cypermethrin in bacteria. Enzyme kinetics of laccase was deduced in the test isolate under normal and pesticide stress conditions. Amplification of laccase gene showed a major band of 1200bp. Maximum copy number of 16S rDNA was seenin uncontaminated soil as compared to pesticide contaminated soil using qRT-PCR. The metagenome sequencing revealed reduction in the population of proteobacteria in contaminated soil as compared to uncontaminated soil but showed dominance of actinobacteria, firmicutes and bacteriodates in pesticide spiked soil. Presence of some new phyla like chloroflexi, planctomycetes, verrucomicrobia was observed followed by extinction of acidobacteria and crenarchaeota in spiked soil. The present study highlights on the potential of 2D bacterial strain i.e., high tolerance level of pesticide, effective biodegradation rate, and presence of laccase gene in bacterial strain 2D, could become a potential biological agent for large-scale treatment of mixture of pesticide (cypermethrin, fipronil, imidacloprid and sulfosulfuron) in natural environment (soil and water).

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Section: Discussionmentioning

confidence: 99%

Differential analysis of pesticides biodegradation in soil using conventional and high-throughput technology

Gangola

Joshi²,

Kumar

et al. 2021

Preprint

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“…These microorganisms are able to produce ligninolytic enzymes, of which laccase (Lac, EC 1.10.3.2), manganese-dependent peroxidase (MnP, EC 1.11.1.13), and lignin peroxidase are the most important (LiP, EC 1.11.1.14). Because of their low substrate specificity, these enzymes catalyze not only the oxidation of lignin, but also the oxidation of a wide range of structurally varied chemicals that are difficult to degrade, such as PAHs [ 16 , 17 , 18 , 19 ], synthetic dyes [ 20 , 21 ], chlorinated organic compounds [ 22 ], pesticides [ 23 , 24 ], or residues of drugs [ 25 ]. Because of their low substrate specificity, these proteins are also beneficial in the decomposition of creosote.…”

Section: Introductionmentioning

confidence: 99%

PUF-Immobilized Bjerkandera adusta DSM 3375 as a Tool for Bioremediation of Creosote Oil Contaminated Soil

Struszczyk‐Świta

Drożdżyński

Murawska

et al. 2022

IJMS

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Creosote oil, a byproduct of coal distillation, is primarily composed of aromatic compounds that are difficult to degrade, such as polycyclic aromatic hydrocarbons, phenolic compounds, and N-, S-, and O-heterocyclic compounds. Despite its toxicity and carcinogenicity, it is still often used to impregnate wood, which has a particularly negative impact on the condition of the soil in plants that impregnate wooden materials. Therefore, a rapid, effective, and eco-friendly technique for eliminating the creosote in this soil must be developed. The research focused on obtaining a preparation of Bjerkandera adusta DSM 3375 mycelium immobilized in polyurethane foam (PUF). It contained mold cells in the amount of 1.10 ± 0.09 g (DW)/g of the carrier. The obtained enzyme preparation was used in the bioremediation of soil contaminated with creosote (2% w/w). The results showed that applying the PUF-immobilized mycelium of B. adusta DSM 3375 over 5, 10, and 15 weeks of bioremediation, respectively, removed 19, 30, and 35% of creosote from the soil. After 15 weeks, a 73, 79, and 72% level of degradation of fluoranthene, pyrene, and fluorene, respectively, had occurred. The immobilized cells have the potential for large-scale study, since they can degrade creosote oil in soil.

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