Studies on Phylogeny of Chaetomium Species of India

Sekhar, V. Chandra; Prameeladevi, T.; Kamil, Deeba; Ram, Dama

doi:10.20546/ijcmas.2018.708.337

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…DNA bands (PCR products) in the gel were shown. The results revealed that molecular confirmation of C. globosum using the universal primers ITS1 and ITS4, which had an amplicon of 500-560 bp, [22] also reported After BLAST analysis, the sample showed 100% similarity with Chaetomium spp. strain CBS 105.40 with accession number MH856051.1.…”

Section: Molecular Identification Of the Fungal Sample Based Onmentioning

confidence: 78%

“…DNA bands (PCR products) in the gel were shown. The results revealed that molecular confirmation of C. globosum using the universal primers ITS1 and ITS4, which had an amplicon of 500-560 bp, [ 22 ] also reported that with the combination of universal primers, ITS1 and ITS4, sequences amplicon sizes of 500-560 bp were observed. After BLAST analysis, the sample showed 100% similarity with Chaetomium spp.…”

Section: Resultsmentioning

confidence: 98%

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GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

Kumari

Attri

et al. 2022

BioMed Research International

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To discover new natural resources with biological effects, the chemical investigation of antioxidant and antimicrobial activities of extract’s Chaetomium globosum isolated from roots of Urginea indica. Gas chromatography-mass spectrometry (GC-MS) analysis demonstrated the presence of the major chemical constituents present in the methanol extract (1,3-oxathiolane, 1,3-cyclopentadiene, 5-(1-methylethylidene), 5,9-hexadecadienoic acid, methyl ester, decane), chloroform extract (acetic acid, diethoxy-, ethyl ester, 2,2-bis(ethylsulfonyl)propane, 3-methyl-2-(2-oxopropyl) furan), and hexane extract (3-hexanone, 4,4-dimethyl, decane,2,6-dimethyldecane, decane, 2,4,6-trimethyl, decane, 2,4,6-trimethyl, 1-butanesulfinamide, 1,1,2,2,3,3,4,4,4-nonafluoro-N-methyl, decane). The total compound identified (56.2%) in chloroform extract, (54.72%) in hexane extract, and (65%) in methanol extract. The antioxidant effects were performed using diphenylpicrylhydrazyl radical (DPPH). The results showed that the methanol extract showed significantly the highest anti-DPPH with an IC50 value of 37.61 ± 1.37 μg/mL, followed by chloroform and hexane extracts with IC50 values of 40.82 ± 3.60 and 45.20 ± 2.54 μg/mL, respectively. The antifungal activity of extracts was evaluated against pathogens fungi including Fusarium oxysporum, Rosellinia necatrix, Cladosporium xanthochromaticum, and Sclerotinia sclerotiorum. Methanolic and chloroform extracts showed maximum inhibition against all test pathogens, while hexane extract showed minimum inhibition.

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Section: Molecular Identification Of the Fungal Sample Based Onmentioning

confidence: 78%

“…DNA bands (PCR products) in the gel were shown. The results revealed that molecular confirmation of C. globosum using the universal primers ITS1 and ITS4, which had an amplicon of 500-560 bp, [ 22 ] also reported that with the combination of universal primers, ITS1 and ITS4, sequences amplicon sizes of 500-560 bp were observed. After BLAST analysis, the sample showed 100% similarity with Chaetomium spp.…”

Section: Resultsmentioning

confidence: 98%

GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

Kumari

Attri

et al. 2022

BioMed Research International

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“…The results presented here confirmed the variations between species level of C.globosum. Chandra, et al [24] studied forty four isolates of Chaetomium (fifteen isolates C. globosum) and subjected them to PCR amplification of the genes; β-tubulin, actin, calmodulin, tef-1 and rpb2. The sequences were subjectedto maximum parsimony phylogenetic analysis.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Cellulolytic Capacity of Chaetomium globosum Isolates

El‐Dawy

Shebany

Gherbawy

2020

ARRB

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Chaetomium species are saprophytic fungi, havethe ability to degrade cellulose with hydrolytic enzymes. Hydrolytic enzymes are a cluster of enzymes, which have the flexibility of breaking down complicated molecules into smaller molecules. The objective of this study was to clarify the genetic diversity among the isolated Chaetomium population by using PCR-based methods, along with the study of their cellulolytic activities. Chaetomium spp. were isolated from twenty-eight samples of Cuminum cyminum and Pimpinella anisum, and identified morphologically and molecularly by ITS1 and ITS4 primers. The sequencing indicated that all the isolates of Chaetomium had a 99% sequence identity with Chaetomium globosum sequences from GenBank. Molecular techniques with Internal Transcribed Spacer (ITS) region sequencing and specific genes random primers polymerase chain reaction (SGRP-PCR) showed the existence of high DNA polymorphism of Chaetomium globosum. All isolates were tested for their ability of cellulases production. Nine of the thirteen isolates of Chaetomium globosumcould produce cellulases. There are variations between C. globosum isolates and the source of the isolates and their habitat do not correlate to their ability to secrete cellulases.

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“…For this reason, identification on the base of molecular and sequence analysis is needed as some species may lack reproductive structures production over time 'Aini et al 2022). Over the past few years, many studies have been conducted to group, classify, and accurately identify different species of Chaetomium, utilizing DNA sequencing as a key tool (Sekhar et al 2018). Very limited studies have been conducted in Arabic countries and Iraq for the genetic characterization of Chaetomium species (Abdullah et al 2015;Abdel-Azeem 2020;Al-Dossary et al 2021;Alfartosy et al 2021).…”

Section: Introductionmentioning

confidence: 99%

New endophytic fungal species of Chaetomiaceae (Ascomycota) in Iraq

AL-RIFAIE,

AMEEN

2023

Biodiversitas

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Abstract. Al-Rifaie AA, Ameen MKM. 2023. New endophytic fungal species of Chaetomiaceae (Ascomycota) in Iraq. Biodiversitas 24: 5270-5277. The present study was conducted to isolate and identify some endophytic fungi from vegetable crops in Iraq. Samples of eight vegetable plants, namely Abelmoschus esculentus, Mentha piperita, Vicia faba, Petroselinum sativum, Ocimum basilicum, Lawsonia inermis, Beta vulgaris and Apium graveolens were collected from three regions in Basrah (Abu Al-Khaseeb, Karmat Ali and the Centre of Basrah). Isolation was done by solid culture method and moist culture method. The recovered endophytic fungi were purified and axenic cultures of each isolated species were then identified on the basis of their macro and micro-morphological features. Morphological identification was further confirmed by molecular analysis through DNA extraction and sequencing by PCR amplification of the ITS4 and ITS5 gene primers. Phylogenetic examination revealed that five novel endophytic fungal species related to the family Chaetomiaceae were isolated from vegetable plants, including Chaetomium cucumericola, C. madrasense, Amesia atrobrunnea, A. cymbiformis and Botryotichum verrucosum. Three species, including C. cucumericola, A. cymbiformis and B. verrucosum, were documented for the first time in the Iraqi mycobiota. To the best of our acknowledgment, this study is the first to investigating endophytic fungi from vegetables in Iraq.

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Studies on Phylogeny of Chaetomium Species of India

Cited by 3 publications

References 9 publications

GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

Molecular Characterization and Cellulolytic Capacity of Chaetomium globosum Isolates

New endophytic fungal species of Chaetomiaceae (Ascomycota) in Iraq

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