Studies on Physical and Chemical Quality Evaluation of Crude Drugs Preparations. I. Analysis of Pueraria Radix and Species Puerariae

Hayakawa, Junko; Noda, Naoki; Yamada, Sadaji; Uno, Keiichi

doi:10.1248/yakushi1947.104.1_50

Cited by 16 publications

(7 citation statements)

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“…Puerarin is known as an isoflavone ( Fig. 1d ) that is found in a number of plants and herbs such as the root of pueraria 14 . Puerarin at the concentrations of 100 μM significantly prolonged APD of rat ventricular myocytes; whereas isoproterenol (10 nM) significantly shortened APD of rat ventricles, comparing with control ( Fig.…”

Section: Resultsmentioning

confidence: 99%

The Effects of Puerarin on Rat Ventricular Myocytes and the Potential Mechanism

Zhao²,

Liang

et al. 2016

Sci Rep

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Puerarin, a known isoflavone, is commonly found as a Chinese herb medicine. It is widely used in China to treat cardiac diseases such as angina, cardiac infarction and arrhythmia. However, its cardioprotective mechanism remains unclear. In this study, puerarin significantly prolonged ventricular action potential duration (APD) with a dosage dependent manner in the micromolar range on isolated rat ventricular myocytes. However, submicromolar puerarin had no effect on resting membrane potential (RMP), action potential amplitude (APA) and maximal velocity of depolarization (Vmax) of action potential. Only above the concentration of 10 mM, puerarin exhibited more aggressive effect on action potential, and shifted RMP to the positive direction. Millimolar concentrations of puerarin significantly inhibited inward rectified K+ channels in a dosage dependent manner, and exhibited bigger effects upon Kir2.1 vs Kir2.3 in transfected HEK293 cells. As low as micromolar range concentrations of puerarin significantly inhibited Kv7.1 and IKs. These inhibitory effects may due to the direct inhibition of puerarin upon channels not via the PKA-dependent pathway. These results provided direct preclinical evidence that puerarin prolonged APD via its inhibitory effect upon Kv7.1 and IKs, contributing to a better understanding the mechanism of puerarin cardioprotection in the treatment of cardiovascular diseases.

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Section: Resultsmentioning

confidence: 99%

The Effects of Puerarin on Rat Ventricular Myocytes and the Potential Mechanism

Zhao²,

Liang

et al. 2016

Sci Rep

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“…Puerariae flavonoids were prepared in our laboratory, with match number TH-0108 (flavonoid purity of 82%, puerarin content 32.0%). Other components in puerariae flavonoids, such as daidzein, daidzin and daidzin-4′,7-diglucoside, were soluble in water, methanol and acetonitrile (Cao et al, 1999;Hayakawa et al, 1984). Phosphoric acid was analytical grade and methanol and acetonitrile (HPLC grade) were purchased from Fisher Scientific (New Jersey, USA).…”

Section: Methodsmentioning

confidence: 99%

Determination of puerarin in rat cortex by high‐performance liquid chromatography after intravenous administration of Puerariae flavonoids

Yan

Wang

Zhang

et al. 2005

Biomedical Chromatography

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In the present study, a rapid and simple high-performance liquid chromatographic (HPLC) assay for determination of puerarin in rat cortex was developed. The analysis was carried out on a Zorbax SB-C18 column with mobile phase acetonitrile-0.5% aqueous phosphoric acid (11:89, v/v). The detection was by UV at 252 nm. The calibration curve for puerarin was linear (r=0.9999) over the concentration range 0.516-206.250 microg/mL. The limit of detection was 0.206 microg/mL (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) was 0.516 microg/mL. Stability studies showed that puerarin was stable at temperatures of 4 degrees C in methanol for at least 30 days. The intra- and inter-day assays of puerarin from rat cortex were less than 2.5% at concentration range 0.516-206.250 microg/mL and good overall recoveries (97.4-101.7%) were found at same concentrations. The method was applied to determine the pharmacokinetic parameters and the time course of puerarin in rat cortex, following a single dosage of intravenous administration of flavonoids from Puerariae radix at 32 mg/kg of puerarin to male Wistar rats.

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“…Herbal medicines are derived from various plants and have been prescribed to treat many diseases for thousands of years. It was previously reported that phytoestrogens, such as daidzein and daidzin, can be found in puerariae radix [ 11 ], and the glabridin present in licorice roots was suggested to be a useful treatment for post-menopausal women because of its estrogenic activity [ 12 – 14 ]. Herbal medicines have recently been used as an alternative to estrogen therapy for menopausal syndrome, but little is known about the pharmacological activity of such medicines.…”

Section: Introductionmentioning

confidence: 99%

The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them

Wang

Kanda

Shimono

et al. 2018

BMC Complement Altern Med

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BackgroundJapanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito, kamishoyosan, nyoshinsan, keishibukuryogan, and tokishakuyakusan).MethodsWe evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay.ResultsWe found that unkeito, kamishoyosan, and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito. We found that glycyrrhizae radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and glycyrrhizae radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity.ConclusionIt was suggested that unkeito, kamishoyosan, and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito.

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Studies on Physical and Chemical Quality Evaluation of Crude Drugs Preparations. I. Analysis of Pueraria Radix and Species Puerariae

Cited by 16 publications

References 0 publications

The Effects of Puerarin on Rat Ventricular Myocytes and the Potential Mechanism

The Effects of Puerarin on Rat Ventricular Myocytes and the Potential Mechanism

Determination of puerarin in rat cortex by high‐performance liquid chromatography after intravenous administration of Puerariae flavonoids

The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them

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