Studies on poly ADPR polymerase using the specific antibody

Okazaki, H.; Delaunoy, Jean‐Pierre; Hog, F.; Bilen, Julide; Niedergang, Claude; Creppy, E. E.; Ittel, Marie‐Elisabeth; Mandel, P.

doi:10.1016/s0006-291x(80)80037-4

Cited by 10 publications

(1 citation statement)

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“…The work presented in this paper provides experimental evidence for the develeopment of specific antibodies in mice against the NHP, poly (ADP-Rib) polymerase. Recently Ikai et al (13) and Okazaki et al (14) have also reported on the isolation of antibodies to calf thymus polymerase. The availability of specific antibodies to chromosomal proteins with established enzymatic activities should greatly accelerate the elucidation of their distributions and functions in chromatin.…”

Section: Discussionmentioning

confidence: 99%

Antibody to poly(adenosine diphosphate-ribose) polymerase and its use in chromfltin analysis

1982

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To facilitate investigations on the organization of poly (ADP-Rib) polymerase in chromatin, and to elucidate its biological function, polymerase purified from HeLa nuclei was used to elicit antibodies in mice. The anti-polymerase sera was found to be specific by multiple criteria. The association of polymerase with oligonucleosomes of differing chain size was determined by the specific binding of polymerase antibody (and as control, anti-histone H3) to nitrocellulose transfers of native electrophoretic gels of these particles. By this technique, polymerase appears to be associated with a select subclass of nucleosomes. Further resolution of the polymerase binding sites within nucleosome classes was achieved with the antibody by two-dimensional native polyacrylamide gel electrophoresis and nitrocellulose transfer.

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Section: Discussionmentioning

confidence: 99%